3-Phenylpropan-1-Amine Enhanced Susceptibility of Serratia marcescens to Ofloxacin by Occluding Quorum Sensing

Yin, Lujun; Zhang, Pingping; Wang, Wei; Tang, Shi; Deng, Shi-Ming; Jia, Ai‐Qun

doi:10.1128/spectrum.01829-22

Cited by 10 publications

(6 citation statements)

References 43 publications

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“… 40 Past studies reported that S. marcescens enhanced its infectivity and its inherent resistance to antibiotics through plasmids and efflux pumps, which greatly contributed to the emergence of multiple drug-resistant strains and finally complicated the treatment process, 41 , 42 and the underlying mechanisms were complicated. 43 In our study, regardless of the two S. marcescens isolates resistant to cefazolin, meropenem, and chloramphenicol simultaneously, the efflux pump-related genes sdeB, sdeD, sdeR, sdeS , s deY , or hasF (in combination) were observed in all β-lactams and chloramphenicol-resistant isolates, respectively. Efflux pump is helpful for bacteria to excrete antibiotics, and it is the main cause of clinical intrinsic and acquired multidrug resistance in bacteria.…”

Section: Discussionmentioning

confidence: 45%

Prevalence and Characterization of Serratia marcescens Isolated from Clinical Bovine Mastitis Cases in Ningxia Hui Autonomous Region of China

Zhong¹,

Shen²,

Liu³

et al. 2023

IDR

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Purpose This study aimed to investigate the prevalence and genetic characterization of Serratia marcescens isolates from clinical bovine mastitis in Ningxia Hui Autonomous Region of China. Methods S. marcescens was identified by the polymerase-chain reaction of 16S rRNA gene and sequencing. Antimicrobial susceptibility was tested by the disk diffusion method. Genes of resistance and virulence were determined by the PCR. Results Overall, S. marcescens were confirmed from 32 of 2897 (1.1%) mastitis milk samples. These isolates showed high resistance to cefazolin (30/32, 93.8%) and chloramphenicol (28/32, 87.5%). A 12.5% (4/32) of the isolates displayed multidrug resistance (MDR). The most prevalent resistant genes found in S. marcescens were TEM (32/32, 100%) and CTX-M (24/32, 75.0%; CTX-M-15 , 14/32, 43.8%; CTX-M-14 , 8/32, 25.0%; CTX-M-65 , 2/32, 6.3%) for extended-spectrum beta-lactamase, cmlA (28/32, 87.5%) and floR (16/32, 50.0%) for chloramphenicol resistance, SIM-1 (2/32, 6.3%) for carbapenemases, and sdeB (28/32, 87.5%), sdeY (26/32, 81.3%), sdeR (26/32, 81.3%) and sdeD (20/32, 62.5%) for efflux pumps. Moreover, all isolates carried virulence genes flhD, entB , and kpn , and most of them contained mrkD (30/32, 93.8%), ycfM (26/32, 81.3%), bsmB (26/32, 81.3%), pigP (26/32, 81.3%), kfu (24/32, 75.0%) and shlB (24/32, 75.0%). Conclusion To our knowledge, this is the first report of genetic determinants for antimicrobial resistance and virulence in S. marcescens isolated from bovine mastitis cases in China. These findings are useful for developing strategies for prevention and treatment of bovine mastitis caused by S. marcescens in China.

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Section: Discussionmentioning

confidence: 45%

Prevalence and Characterization of Serratia marcescens Isolated from Clinical Bovine Mastitis Cases in Ningxia Hui Autonomous Region of China

Zhong¹,

Shen²,

Liu³

et al. 2023

IDR

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“…Swimming mediated by flagella was decreased by 53% at 400 μg/mL. In a previous study, 3-phenylpropan-1-amine (3-PPA) was found to inhibit S. marcescens NJ01 biofilm formation and virulence factors, such as prodigiosin, protease, lipase, hemolysin, and swimming motility [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…S. marcescens NJ01 forms biofilms through a series of defined stages that culminate in a highly porous, filamentous biofilm composed of cell chains, filaments, and cell clusters dependent on the quorum-sensing systems [ 13 , 39 , 40 ]. Bacteria susceptible to antimicrobial agents can become resistant after forming a biofilm.…”

Section: Discussionmentioning

confidence: 99%

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Cladosporium sphaerospermum extract inhibits quorum sensing associated virulence factors of Serratia marcescens

Liu

Yan

Zou

et al. 2023

Biofilm

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“…Phenylpropane-1-amine (3-PPA) is known to be an antibiotic adjuvant that interferes with QS and disrupts signaling between bacteria without posing a threat to the bacteria themselves, potentially resolving resistance in pathogenic bacteria [6]. In this recent and unique study, 3-phenylpropan-1-amine (3-PPA) was determined to inhibit biofilm formation.…”

Section: Anti-biofilm Activity Of Peptides and Organic Compounds 21 A...mentioning

confidence: 99%

Efficacy of Natural and Synthetic Biofilm Inhibitors Associated with Antibiotics in Eradicating Biofilms Formed by Multidrug-Resistant Bacteria

Kloula Ben Ghorbal,

Dhaouadi,

Bouzenbila

et al. 2024

Recent Advances in Bacterial Biofilm Studies - Formation, Regulation, and Eradication in Human Infections

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Biofilms formed by multidrug resistant (MDR) bacteria like methicillin-resistant Staphylococcus aureus (MRSA) and others are the main causes of infections that represent a serious public health issue. Persistent MDR infections are mostly derived from biofilm formation which in turn leads to resistance to conventional antimicrobial therapy. Inhibition of bacterial surface attachment is the new alternative strategy without affecting the bacterial growth. Thus, the discovery of compounds that interfere with biofilm production, virulence factors release and quorum sensing (QS) detection in pathogens is a promising processus. Among these compounds, natural and synthetic molecules are a compelling alternative to attenuate pathogenicity. The combination of these compounds with antibiotics makes the bacteria more vulnerable to the later, once used alone. This combination can restore antibiotic effectiveness against MDR bacteria. Among these molecules, 3-phenylpropan-1-amine (3-PPA) has been found to inhibit Serratia marcescens biofilm formation, PAβN has been proven to inhibit biofilm prodcution in A. baumannii, while brominated Furanone C-30 has been reported to be a potent inhibitor of the QS system and P. aeruginosa biofilm. Therefore, the combination between biofilm-inhibitors and antibiotics represents a promising strategy to mitigate antibiotic resistance in MDR pathogens, which has become a major threat to public healthcare around the globe.

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3-Phenylpropan-1-Amine Enhanced Susceptibility of Serratia marcescens to Ofloxacin by Occluding Quorum Sensing

Abstract: Multidrug-resistant bacteria have become a major threat to global public health, leading to increased morbidity, mortality, and health care costs. Bacterial virulence factors and biofilms, which are regulated by quorum sensing (QS), are the primary causes of multidrug resistance.

Cited by 10 publications

References 43 publications

Prevalence and Characterization of Serratia marcescens Isolated from Clinical Bovine Mastitis Cases in Ningxia Hui Autonomous Region of China

Prevalence and Characterization of Serratia marcescens Isolated from Clinical Bovine Mastitis Cases in Ningxia Hui Autonomous Region of China

Cladosporium sphaerospermum extract inhibits quorum sensing associated virulence factors of Serratia marcescens

Efficacy of Natural and Synthetic Biofilm Inhibitors Associated with Antibiotics in Eradicating Biofilms Formed by Multidrug-Resistant Bacteria

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