3-Phosphohistidine and 6-Phospholysine Are Substrates of a 56-kDa Inorganic Pyrophosphatase from Bovine Liver

Evidence for HTR1A and LHPP as interacting genetic risk factors in major depression

“…29 Lhpp has been characterized in vitro as efficiently catalyzing the hydrolysis of P-N bonds in phosphohistidine and phospholysine, and less efficiently catalyzing the hydrolysis of P-N or P-O bonds in imidodiphosphate and pyrophosphate, respectively. 30 Expression of a 1.8 kb LHPP mRNA was previously observed in brain, liver and kidney. 29 Using a probe from the 3 0 end of LHPP mRNA, we detected fulllength LHPP mRNA as an approximately 1.7 kb transcript in multiple tissues.…”

“…These transcripts are shown in Figure 4. Expression levels were categorized according to threshold cycle: very high ( < 20), high (20)(21)(22)(23)(24)(25), moderate (25)(26)(27)(28)(29)(30), low (30)(31)(32)(33)(34)(35), very low/questionable (35)(36)(37)(38)(39)(40) or not detected (no signal above background after 40 cycles). Because of the linkage and association of LHPP variants to MDD, there was particular focus on measuring expression of these transcripts in the brain (Figure 3b).…”

Advances in development of new tools for the study of phosphohistidine

“…28 In this particular case, it is not clear which isomer of pHis is formed and on which His residue. Mammalian pHis phosphatases, which have been characterized include: protein pHis phosphatase 1 (PHPT1); 17,[29][30][31][32][33][34][35] Lys/His phosphatase (LHPPase); 36,37 Ser Thr protein phosphatases (PP1/2 A/2C); 38,39 T-cell ubiquitin ligand-2 (TULA-2); 40,41 and the recently reported phosphoglycerate mutase-5 (PGAM5). 42 In addition, pHis phosphatase activity has been reported in rat tissue extracts but these have not been fully characterized.…”

The synthesis of imidodiphosphoric acid derivatives as potential substrates in the pyrophosphorolysis reaction

“…Moreover, the dissociation constants for both substances are also close [4,5]. HO Imidodiphosphoric acid is a substrate for both prokaryotic pyrophosphatase from E. coli and eukaryotic pyrophosphatase from bovine liver [8,9]. Moreover, it was shown that acid ( II ) can replace inorganic pyrophosphate in the pyrophosphorolysis reaction catalyzed by RT from avian myeloblastosis virus and by a number of DNA polymerases [10] and also inhibit Sadenosylmethionine synthase [11].…”