3′ Untranslated Region-Dependent Degradation of the aceA mRNA, Encoding the Glyoxylate Cycle Enzyme Isocitrate Lyase, by RNase E/G in Corynebacterium glutamicum

Abstract

We previously reported that the Corynebacterium glutamicum RNase E/G encoded by the rneG gene (NCgl2281) is required for the 5= maturation of 5S rRNA. In the search for the intracellular target RNAs of RNase E/G other than the 5S rRNA precursor, we detected that the amount of isocitrate lyase, an enzyme of the glyoxylate cycle, increased in rneG knockout mutant cells grown on sodium acetate as the sole carbon source. Rifampin chase experiments showed that the half-life of the aceA mRNA was about 4 times longer in the rneG knockout mutant than in the wild type. Quantitative real-time PCR analysis also confirmed that the level of aceA mRNA was approximately 3-fold higher in the rneG knockout mutant strain than in the wild type. Such differences were not observed in other mRNAs encoding enzymes involved in acetate metabolism. Analysis by 3= rapid amplification of cDNA ends suggested that RNase E/G cleaves the aceA mRNA at a single-stranded AU-rich region in the 3= untranslated region (3=-UTR). The lacZ fusion assay showed that the 3=-UTR rendered lacZ mRNA RNase E/G dependent. These findings indicate that RNase E/G is a novel regulator of the glyoxylate cycle in C. glutamicum.

The hmsT 3′ untranslated region mediates c‐di‐GMP metabolism and biofilm formation in Yersinia pestis

“…aureus and S . enteric (Maeda and Wachi, ; Ruiz de los Mozos et al ., ; Lopez‐Garrido et al ., ). The work reported here reveals that the 3 ′ UTR of hmsT mRNA is a negative modulator of hmsT mRNA in Y. pestis .…”

RNase III mediated cleavage of the coding region of mraZ mRNA is required for efficient cell division in Corynebacterium glutamicum

“…Similar observations were reported that several E. coli mRNAs, such as those of the betT , mltD and bdm genes, are subjected to RNase III cleavage in their coding region (Sim et al ., ; Lim et al ., ; Sim et al ., ). In the case of C. glutamicum , the degradation of aceA mRNA by RNase E/G is 3′‐UTR dependent (Maeda and Wachi, ). In C. glutamicum , many mRNAs (33%) were identified to be leaderless mRNAs (Pfeifer‐Sancar et al ., ).…”

“…C. glutamicum has one RNase E/G ortholog and one RNase J ortholog, but no RNase Y (Maeda and Wachi, 2012b). Our previous study showed that RNase E/G is involved in the regulation of glyoxylate cycle and FMN riboswitch in C. glutamicum (Maeda and Wachi, 2012a;Takemoto et al, 2015). Therefore, it is possible that RNase E/G and/or RNase J play a central role in mRNA metabolism in C. glutamicum.…”

Metabolic engineering of Bacillus subtilis for growth on overflow metabolites

“…Results of this study indicate a control of the aceBA -operon of B. licheniformis in B. subtilis at the transcriptional level . A transcriptional regulation of the glyoxylate shunt has also been shown for the Gram-positive bacterium Corynebacterium glutamicum , where the expression of the malate synthase and isocitrate lyase is positively controlled by the LuxR-type transcriptional regulator RamA in the presence of acetate [15]. Voigt et al detected a strong induction of the AceB expression in B. licheniformis at the transcriptional and translational level as soon as the preferred carbon and energy source glucose is exhausted [16].…”