[30] Detection of conjugated dienes by second derivative ultraviolet spectrophotometry

Corongiu, Francesco P.; Banni, Sebastiano

doi:10.1016/s0076-6879(94)33033-6

Cited by 123 publications

(88 citation statements)

References 10 publications

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“…Lipids were extracted with chloroform:methanol (2:1, v/v), dried under N 2 gas, and resuspended in PBS. Lipid oxidation was assessed by quantifying conjugated dienes from the secondary derivative of the UV spectrum around 230 nm using (Ϯ)9-HODE as a standard (31). Approximately 15-18% of PLPS fatty acid chains were estimated to be oxidized by the AAPH treatment, which is similar to the level achieved by others (32) while no conjugated dienes were detected in DOPC or DOPS treated with AAPH.…”

Section: Preparation Of Phospholipid Vesiclessupporting

confidence: 63%

Auto-Oxidation and Oligomerization of Protein S on the Apoptotic Cell Surface Is Required for Mer Tyrosine Kinase-Mediated Phagocytosis of Apoptotic Cells

Uehara

Shacter

2008

The Journal of Immunology

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Prompt phagocytosis of apoptotic cells prevents inflammatory and autoimmune responses to dying cells. We have previously shown that the blood anticoagulant factor protein S stimulates phagocytosis of apoptotic human B lymphoma cells by human monocyte-derived macrophages. In this study, we show that protein S must first undergo oxidative activation to stimulate phagocytosis. Binding of human protein S to apoptotic cells or to phosphatidylserine multilamellar vesicles promotes auto-oxidation of Cys residues in protein S, resulting in covalent, disulfide-linked dimers and oligomers that preferentially bind to and activate the human Mer tyrosine kinase (MerTK) receptor on the macrophages. The prophagocytic activity of protein S is eliminated when disulfide-mediated oligomerization is prevented, or when MerTK is blocked with neutralizing Abs. Protein S oligomerization is independent of phospholipid oxidation. The data suggest that membranes containing phosphatidylserine serve as a scaffold for protein S-protein S interactions and that the resulting auto-oxidation and oligomerization is required for the prophagocytic activity of protein S. In this way, apoptotic cells facilitate their own uptake by macrophages. The requirement for oxidative modification of protein S can explain why this abundant blood protein does not constitutively activate MerTK in circulating monocytes and tissue macrophages.

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Section: Preparation Of Phospholipid Vesiclessupporting

confidence: 63%

Auto-Oxidation and Oligomerization of Protein S on the Apoptotic Cell Surface Is Required for Mer Tyrosine Kinase-Mediated Phagocytosis of Apoptotic Cells

Uehara

Shacter

2008

The Journal of Immunology

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“…The method is used for determination of a non-specific lipid peroxidation caused by free radicals in biological samples, and is successfully used in the study of peroxidation in isolated lipoprotein fractions (LDL lipoproteins) [45]. However, its use in the direct analysis of plasma is controversial because of the presence of interfering substances, such as heme proteins, purines or pyrimidines in the UV region measurement [42,57]. Increased sensitivity of the method can be achieved by an extraction of lipids into organic solvents in combination HPLC with UV detection [34,58].…”

Section: Conjugated Dienesmentioning

confidence: 99%

Automation of Methods for Determination of Lipid Peroxidation

Sochor¹,

Ruttkay-Nedecký²,

Babula³

et al. 2012

Lipid Peroxidation

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“…As previously reported, CDs were measured by second-derivative spectrophotometry (15,19), which allows great sensitivity, because the CD shoulder that appears in the ordinary spectrum translates into sharp minima peaks, which are more easily measurable. Furthermore, this technique permits a discrimination between the different CD structures (e.g., cis-trans and trans-trans conformation) present in the sample (20).…”

Section: Cdsmentioning

confidence: 99%

“…The CD ratio measurement at 246-and 232-nm minima of the derivative spectra, which can provide an indirect evaluation of plasma antioxidant capacity, was then calculated (19,20). Intra-and interassay coefficients of variation for this method were 8.2 and 11.4%, respectively.…”

Section: Cdsmentioning

confidence: 99%

Early Increase of Oxidative Stress and Reduced Antioxidant Defenses in Patients With Uncomplicated Type 1 Diabetes

et al. 2002

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OBJECTIVE -Diabetes increases the risk of coronary heart disease (CHD) to a greater extent in women than in men. We investigated whether type 1 diabetic patients with short duration of disease and without complications have an altered oxidative status and whether there are differences between men and women.RESEARCH DESIGN AND METHODS -We investigated oxidative status in 29 control subjects and 37 patients with uncomplicated type 1 diabetes with duration of 6 Ϯ 3 years.RESULTS -Compared with control subjects, type 1 diabetic patients had lower total plasma antioxidant capacity (TRAP) (720.3 Ϯ 111.2 vs. 972.5 Ϯ 97.7 mol/l in men, P Ͻ 0.001; 579.8 Ϯ 95.4 vs. 930.1 Ϯ 84.2 in women, P Ͻ 0.001), higher lipid hydroperoxide (ROOH) levels (6.4 Ϯ 2.2 vs. 2.0 Ϯ 0.7 mol/l in men, P Ͻ 0.001; 8.1 Ϯ 1.9 vs. 2.2 Ϯ 0.6 in women, P Ͻ 0.001), higher total conjugated diene (CD) levels (0.037 Ϯ 0.003 vs. 0.033 Ϯ 0.002 A.U. in men, P Ͻ 0.001), lower 246-nm CD levels (0.0032.Ϯ 0.0010 vs. 0.0070 Ϯ 0.0012 A.U. in men, P Ͻ 0.001; 0.0022 Ϯ 0.0011 vs. 0.0072 Ϯ 0.0014 A.U. in women, P Ͻ 0.001), and higher 232-nm CD levels (0.0348 Ϯ 0.0041 vs. 0.0257 Ϯ 0.0022 A.U. in men, P Ͻ 0.001; 0.0346 Ϯ 0.0031 vs. 0.0246 Ϯ 0.0074 A.U. in women, P Ͻ 0.001). Compared with diabetic men, diabetic women had lower TRAP (P Ͻ 0.01), higher ROOH levels (P Ͻ 0.01), and lower 246-nm CD levels (P Ͻ 0.05). Plasma concentration of uric acid was significantly lower in patients with type 1 diabetes than in control subjects (3.3 Ϯ 0.3 vs. 4.3 Ϯ 0.2 mg/dl; P ϭ 0.009) with a significant difference between women and men with type 1 diabetes (2.6 Ϯ 0.3 vs. 3.9 Ϯ 0.3, respectively; P ϭ 0.009).CONCLUSIONS -Our findings suggest that reduced antioxidant activity and increased oxidative stress occur early after the diagnosis of type 1 diabetes, especially in women, and this might explain, at least in part, the increased susceptibility of diabetic women to cardiovascular complications.

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[30] Detection of conjugated dienes by second derivative ultraviolet spectrophotometry

Cited by 123 publications

References 10 publications

Auto-Oxidation and Oligomerization of Protein S on the Apoptotic Cell Surface Is Required for Mer Tyrosine Kinase-Mediated Phagocytosis of Apoptotic Cells

Auto-Oxidation and Oligomerization of Protein S on the Apoptotic Cell Surface Is Required for Mer Tyrosine Kinase-Mediated Phagocytosis of Apoptotic Cells

Automation of Methods for Determination of Lipid Peroxidation

Early Increase of Oxidative Stress and Reduced Antioxidant Defenses in Patients With Uncomplicated Type 1 Diabetes

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