[31] High resolution analysis of carotenoids in human plasma by high-performance liquid chromatography

Handelman, Garry J.; Shen, Binghua; Krinsky, Norman I.

doi:10.1016/0076-6879(92)13135-k

Cited by 56 publications

(24 citation statements)

References 9 publications

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“…Zeaxanthin is specifically concentrated at the macula, whereas lutein is distributed throughout the retina (8,9,20). Dietary lutein and zeaxanthin may prevent agerelated macular degeneration (10), a visual disorder that affects 20% of Americans > 75 y of age (29).…”

Section: Tablementioning

confidence: 99%

“…The enzyme reagent was prepared in 0.1 mol sodium phosphate buffer/L, pH 7.0, with 0.1% Triton-X-100 (Sigma Chemical Co). Samples were allowed to digest at ambient temperature for 1 h. The samples were then mixed with 1 mL ethanol, 100 L 5% sodium dodecyl sulfate, and 50 L internal standard (apo-10-carotenal-O-ethyl-oxime) in methanol (20). After vigorous mixing, the sample was extracted with 2 mL hexane and 2 mL diethyl ether.…”

Section: Analysis Of Plasma Lutein and Zeaxanthinmentioning

confidence: 99%

“…Lutein and zeaxanthin are specifically accumulated in the macular region of the retina (8,9,20), where they bind to the retinal protein tubulin (28). Zeaxanthin is specifically concentrated at the macula, whereas lutein is distributed throughout the retina (8,9,20).…”

Section: Tablementioning

confidence: 99%

“…Plasma carotenoids were analyzed by using a procedure adapted from the method of Handelman et al (20). Positive-displacement pipettors were used to dispense plasma samples and internal standard.…”

Section: Analysis Of Plasma Lutein and Zeaxanthinmentioning

confidence: 99%

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Lutein and zeaxanthin concentrations in plasma after dietary supplementation with egg yolk

Handelman

Nightingale

Lichtenstein

et al. 1999

The American Journal of Clinical Nutrition

243

158

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Background:The food matrix in which carotenoids are found affects their bioavailability. Lutein and zeaxanthin are abundant in egg yolks and accumulate in the macular region of the retina, where they may affect visual function. Objective: We sought to determine whether plasma lutein and zeaxanthin concentrations are elevated after dietary supplementation with egg yolk. Design: Eleven moderately hypercholesterolemic men and women consumed 2 separate baseline diets, which contained 29-33% of energy as total fat, with 20% of energy as either beef tallow or corn oil. These diets were supplemented with cooked chicken egg yolks (1.3 egg yolks/d for an intake of 10.4 MJ). Each subject consumed all 4 diets. Each diet was consumed for 4.5 wk, with a washout period of ≥ 2 wk between diet phases. At the end of each diet phase, fasting morning plasma samples were collected and stored for carotenoid analysis by HPLC. Commercial chicken egg yolks were analyzed for carotenoids and cholesterol. Results: Egg yolk supplementation of the beef tallow diet increased plasma lutein by 28% (P < 0.05) and zeaxanthin by 142% (P < 0.001); supplementation of the corn oil diet increased plasma lutein by 50% (P < 0.05) and zeaxanthin by 114% (P < 0.001). Changes in plasma lycopene and ␤-carotene were variable, with no consistent trend. Egg yolk supplementation increased plasma LDL-cholesterol concentrations by 8-11% (P < 0.05). Conclusions: Egg yolk is a highly bioavailable source of lutein and zeaxanthin. The benefit of introducing these carotenoids into the diet with egg yolk is counterbalanced by potential LDL-cholesterol elevation from the added dietary cholesterol.Am J Clin Nutr 1999;70:247-51.

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Section: Tablementioning

confidence: 99%

Section: Analysis Of Plasma Lutein and Zeaxanthinmentioning

confidence: 99%

Section: Tablementioning

confidence: 99%

Section: Analysis Of Plasma Lutein and Zeaxanthinmentioning

confidence: 99%

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Lutein and zeaxanthin concentrations in plasma after dietary supplementation with egg yolk

Handelman

Nightingale

Lichtenstein

et al. 1999

The American Journal of Clinical Nutrition

243

158

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“…Erythrocyte riboflavin was measured by using HPLC (1100 Series HPLC; Agilent Technologies Inc, Palo Alto, CA); plasma retinol concentrations (20,21) were measured by using a Class VP HPLC (Shimadzu, Columbia, MD). The within-day CVs of analytes in pooled, control samples were ͨ5.4% for plasma retinol and ͨ10.0% for erythrocyte riboflavin.…”

Section: Sample Collection and Laboratory Analysesmentioning

confidence: 99%

Supplementation with iron and riboflavin enhances dark adaptation response to vitamin A–fortified rice in iron-deficient, pregnant, nightblind Nepali women

Graham

Haskell

Pandey

et al. 2007

The American Journal of Clinical Nutrition

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Background: Nightblindness affects 16 -52% of pregnant women in areas of Nepal and in some cases persists after vitamin A treatment. Iron and riboflavin affect vitamin A utilization and photoreceptor function, respectively, and pilot data in the study population showed a high prevalence of iron and riboflavin deficiencies. Objective: The objective was to assess the effect of supplemental iron and riboflavin on pupillary threshold (PT) and plasma retinol in nightblind, pregnant Nepali women given vitamin A-fortified rice. Design: Nightblind pregnant women were randomly assigned to receive, 6 d/wk under supervision for 6 wk, a vitamin A-fortified rice curry dish providing 850 g retinal activity equivalents/d with either a 30-mg Fe and 6-mg riboflavin (FeR ѿ VA) capsule or a placebo control (VA only) capsule. Hemoglobin, erythrocyte riboflavin, and plasma ferritin and retinol were measured before and after the intervention. Dark adaptation was assessed by PT score. Results: Women who were iron deficient at baseline (n ҃ 38) had significantly greater improvement in PT score with iron and riboflavin supplementation than without (P ҃ 0.05). Iron and riboflavin supplements significantly reduced the prevalences of riboflavin deficiency (from 60% to 6%; P 0.0001), iron deficiency anemia (from 35% to 15%; P 0.007), and abnormal PT (from 87% to 30%; P 0.05) from baseline. Mean increases in erythrocyte riboflavin (P 0.0001) and plasma ferritin (P ҃ 0.01) were greater in the FeR ѿ VA group than in the VA only group. Conclusions: Iron deficiency may limit the efficacy of vitamin A to normalize dark adaptation in pregnant Nepali women. Further studies are needed to assess the effect of simultaneous delivery of iron and vitamin A for the treatment of nightblindness.Am J Clin Nutr 2007;85: 1375-84.

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Distribution of Lutein and Zeaxanthin Stereoisomers in the Human Retina

Bone

Landrum

Friedes

et al. 1997

Experimental Eye Research

371

273

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[31] High resolution analysis of carotenoids in human plasma by high-performance liquid chromatography

Cited by 56 publications

References 9 publications

Lutein and zeaxanthin concentrations in plasma after dietary supplementation with egg yolk

Lutein and zeaxanthin concentrations in plasma after dietary supplementation with egg yolk

Supplementation with iron and riboflavin enhances dark adaptation response to vitamin A–fortified rice in iron-deficient, pregnant, nightblind Nepali women

Distribution of Lutein and Zeaxanthin Stereoisomers in the Human Retina

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