31P MRSI Studies in Patients with Cancer

Khlebnikov, Vitaliy; Wijnen, Jannie P.; Kemp, Wybe J. M. van der; Klomp, Dennis W. J.

doi:10.1016/bs.arnmr.2015.08.004

Cited by 9 publications

(14 citation statements)

References 189 publications

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“…However, 31 P MRS suffers from inherently low resolution (typical acquisition volume ¼ 3 cm isotropic), low signalto-noise ratio, and long scan times (upwards of 5 min). Despite being highly specific to pH of the cytosol (19,20), these current limitations make it very challenging for 31 P MRS to map neuronal activity-elicited transient pHi changes.…”

Section: Introductionmentioning

confidence: 99%

Establishing upper limits on neuronal activity–evoked pH changes with APT‐CEST MRI at 7 T

Khlebnikov

Siero

Bhogal

et al. 2017

Magnetic Resonance in Med

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PurposeTo detect neuronal activity–evoked pH changes by amide proton transfer–chemical exchange saturation transfer (APT‐CEST) MRI at 7 T.MethodsThree healthy subjects participated in the study. A low‐power 3‐dimensional APT‐CEST sequence was optimized through the Bloch‐McConnell equations. pH sensitivity of the sequence was estimated both in phantoms and in vivo. The feasibility of pH–functional MRI was tested in Bloch‐McConnell‐simulated data using the optimized sequence. In healthy subjects, the visual stimuli were used to evoke transient pH changes in the visual cortex, and a 3‐dimensional APT‐CEST volume was acquired at the pH‐sensitive frequency offset of 3.5 ppm every 12.6 s.ResultsIn theory, a three‐component general linear model was capable of separating the effects of blood oxygenation level–dependent contrast and pH. The Bloch‐McConnell equations indicated that a change in pH of 0.03 should be measurable at the experimentally determined temporal signal‐to‐noise ratio of 108. However, only a blood oxygenation level–dependent effect in the visual cortex could be discerned during the visual stimuli experiments performed in the healthy subjects.ConclusionsThe results of this study suggest that if indeed there are any transient brain pH changes in response to visual stimuli, those are under 0.03 units pH change, which is extremely difficult to detect using the existent techniques. Magn Reson Med 80:126–136, 2018. © 2017 The Authors Magnetic Resonance in Medicine published by Wiley Periodicals, Inc. on behalf of International Society for Magnetic Resonance in Medicine. This is an open access article under the terms of the Creative Commons Attribution NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Section: Introductionmentioning

confidence: 99%

Establishing upper limits on neuronal activity–evoked pH changes with APT‐CEST MRI at 7 T

Khlebnikov

Siero

Bhogal

et al. 2017

Magnetic Resonance in Med

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“…Moreover, the use of multi‐voxel MRS at 7 T in breast cancer patients showed detection of various phospholipid metabolites such as PCho, GPC, phosphoethanolamine, glycerophosphoethanolamine and nucleotide triphosphates, and their absolute concentrations could be determined . In fact, the reproducibility of PME resonance at 7 T was shown to be similar to tCho detection using 1 H MRS, while the reproducibility of inorganic phosphate (P i ), sum of PMEs and sum of PDEs with 31 P MRS was better than the detection of tCho using 1 H MRS …”

Section: In Vivo Mrs Methods In the Study Of Breast Cancer Metabolismmentioning

confidence: 95%

“…With the availability of higher‐field‐strength magnets for MRI in clinics and the use of efficient dedicated quadrature RF coils for the breast along with editing RF sequences, improved sensitivity of 31 P signals was seen. It is now possible to resolve individual phosphate metabolites such as the combined peak for PME and PDE and other phosphorus resonances . Moreover, the use of multi‐voxel MRS at 7 T in breast cancer patients showed detection of various phospholipid metabolites such as PCho, GPC, phosphoethanolamine, glycerophosphoethanolamine and nucleotide triphosphates, and their absolute concentrations could be determined .…”

Section: In Vivo Mrs Methods In the Study Of Breast Cancer Metabolismmentioning

confidence: 99%

Application of in vivo MR methods in the study of breast cancer metabolism

Jagannathan

2018

NMR in Biomedicine

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In the last two decades, various in vivo MR methodologies have been evaluated for their potential in the study of cancer metabolism. During malignant transformation, metabolic alterations occur, leading to morphological and functional changes. Among various MR methods, in vivo MRS has been extensively used in breast cancer to study the metabolism of cells, tissues or whole organs. It provides biochemical information at the metabolite level. Altered choline, phospholipid and energy metabolism has been documented using proton ( 1 H), phosphorus ( 31 P) and carbon ( 13 C) isotopes. Increased levels of choline-containing compounds, phosphomonoesters and phosphodiesters in breast cancer, which are indicative of altered choline and phospholipid metabolism, have been reported using in vivo, in vitro and ex vivo NMR studies. These changes are reversed on successful therapy, which depends on the treatment regimen given.Monitoring the various tumor intermediary metabolic pathways using nuclear spin hyperpolarization of 13 C-labeled substrates by dynamic nuclear polarization has also been recently reported. Furthermore, the utility of various methods such as diffusion, dynamic contrast and perfusion MRI have also been evaluated to study breast tumor metabolism. Parameters such as tumor volume, apparent diffusion coefficient, volume transfer coefficient and extracellular volume ratio are estimated. These parameters provide information on the changes in tumor microstructure, microenvironment, abnormal vasculature, permeability and grade of the tumor. Such changes seen during cancer progression are due to alterations in the tumor metabolism, leading to changes in cell architecture. Due to architectural changes, the tissue mechanical properties are altered; this can be studied using magnetic resonance elastography, which measures the elastic properties of tissues. Moreover, these structural MRI methods can be used to investigate the effect of therapy-induced changes in tumor characteristics. This review discusses the potential of various in vivo MR methodologies in the study of breast cancer metabolism.

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“…However, with technological developments in radiofrequency (RF) coils, the use of high-field MR scanners etc., it is possible to resolve individual phosphate metabolites [87]. Klomp et al demonstrated the ability to detect in breast cancer patients the various phospholipid metabolites like PCho, GPC, phosphoethanolamine, GPE and NTPs using in vivo localized 31 P MRSI at 7 T [88].…”

Section: Mr Methodologies To Study Breast Tissue Metabolismmentioning

confidence: 99%

Breast Tissue Metabolism by Magnetic Resonance Spectroscopy

Jagannathan

Sharma

2017

Metabolites

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Metabolic alterations are known to occur with oncogenesis and tumor progression. During malignant transformation, the metabolism of cells and tissues is altered. Cancer metabolism can be studied using advanced technologies that detect both metabolites and metabolic activities. Identification, characterization, and quantification of metabolites (metabolomics) are important for metabolic analysis and are usually done by nuclear magnetic resonance (NMR) or by mass spectrometry. In contrast to the magnetic resonance imaging that is used to monitor the tumor morphology during progression of the disease and during therapy, in vivo NMR spectroscopy is used to study and monitor tumor metabolism of cells/tissues by detection of various biochemicals or metabolites involved in various metabolic pathways. Several in vivo, in vitro and ex vivo NMR studies using 1H and 31P magnetic resonance spectroscopy (MRS) nuclei have documented increased levels of total choline containing compounds, phosphomonoesters and phosphodiesters in human breast cancer tissues, which is indicative of altered choline and phospholipid metabolism. These levels get reversed with successful treatment. Another method that increases the sensitivity of substrate detection by using nuclear spin hyperpolarization of 13C-lableled substrates by dynamic nuclear polarization has revived a great interest in the study of cancer metabolism. This review discusses breast tissue metabolism studied by various NMR/MRS methods.

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31P MRSI Studies in Patients with Cancer

Cited by 9 publications

References 189 publications

Establishing upper limits on neuronal activity–evoked pH changes with APT‐CEST MRI at 7 T

Establishing upper limits on neuronal activity–evoked pH changes with APT‐CEST MRI at 7 T

Application of in vivo MR methods in the study of breast cancer metabolism

Breast Tissue Metabolism by Magnetic Resonance Spectroscopy

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