31phosphate Nuclear Magnetic Resonance Spectrometry During Cerebral Hypoxia

Ng, Thian C.; Cavender, J. B.; McKay, Robert D.; Kissin, Igor; Glickson, Jerry D.

doi:10.1097/00000542-198209001-00126

Cited by 5 publications

(1 citation statement)

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“…during normocapnia in the mouse, rat, gerbil and human infant have given values in the range 7-09-7-27, corresponding to P1-PCr chemical shifts in the range 4-88-5-07. (Chance, Radda & Seeley, 1979;Norwood, Norwood, Ingwall, Castanedu & Fossel, 1979;Ackerman et al 1980;Ng, Cavender, McKay, Kissin & Glickson, 1982; Thulborn, du Boulay, Duchen & Radda, BRAIN pHi AND METABOLISM STUDIED BY N.M.R. 1982; Naruse, Takada, Koizuka & Natari, 1983;Benabid, Confort, Decorps, Lebas & Leviel, 1984;Ackerman, Berkjowitz & Deviel, 1984;Hope et al 1984).…”

Section: Brain Phimentioning

confidence: 99%

Brain intracellular pH and metabolism during hypercapnia and hypocapnia in the new‐born lamb.

Cady¹,

Chu²,

Costello³

et al. 1987

The Journal of Physiology

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1. The effects of hypercapnia and hypocapnia on brain intracellular pH (pHi) and metabolism were investigated in new-born lambs under barbiturate anaesthesia. 2. 31P nuclear magnetic resonance (n.m.r.) spectroscopy was used to determine brain pHi and the relative concentrations of compounds containing mobile phosphorus nuclei including phosphocreatine (PCr), nucleoside triphosphates (NTP) and inorganic phosphate (Pi). Simultaneous measurements were made of the molar ratio of glucose to oxygen uptake by the brain. 3. During normocapnia (arterial partial pressure of CO2 Pa, CO2, 39 +/- 1 mmHg mean +/- S.E. of mean, n = 9) brain pHi was 7.13 +/- 0.02. Hypercapnia (Pa, CO2, 98 +/- 3 mmHg) was associated with a fall in brain pHi to 6.94 +/- 0.03 (n = 19, P less than 0.001), whereas no significant change in brain pHi occurred during hypocapnia (Pa, CO2, 16 +/- 1 mmHg; brain pHi 7.15 +/- 0.01). 4. During hypercapnia there was an increase in the ratio of Pi to NTP from 1.09 +/- 0.08 to 1.47 +/- 0.06 (P less than 0.001) and a decrease in the ratio PCr/Pi from 1.60 +/- 0.08 to 0.93 +/- 0.04 (P less than 0.001). There was a linear correlation between Pi/NTP and brain pHi. 5. Alterations in arterial PCO2 had no significant effect on the molar ratio of glucose to oxygen uptake by the brain, which remained close to unity. 6. The change in brain pHi observed during hypercapnia can be accounted for by the known physico-chemical buffering capacity of brain tissue. Homoeostasis of brain pHi during hypocapnia provides further evidence that additional regulatory mechanisms operate in these circumstances. 7. The observed changes in PCr and Pi can be accounted for in part by the [H+] dependence of the creatine kinase reaction.

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Section: Brain Phimentioning

confidence: 99%

Brain intracellular pH and metabolism during hypercapnia and hypocapnia in the new‐born lamb.

Cady¹,

Chu²,

Costello³

et al. 1987

The Journal of Physiology

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Depth pulse sequences for surface coils: spatial localization and T₁ measurements

Glickson

Bendall

1984

Magnetic Resonance in Med

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The depth pulse sequences theta;[2 theta(+/- x, +/- y)]2 and 2 theta;theta(+/- x);[2 theta(+/- x, +/- y)]2 have been implemented with a 20 mm diameter two-turn surface coil operating at 31P resonance (89.96 MHz). In these sequences theta refers to the pi/2 rf pulse at the center of the sensitive region of the coil, +/- x and +/- y denote the four orthogonal phases of the rf pulses, and ";" represents an optional brief delay (e.g., 4 microseconds) between pulses to facilitate switching between different phases. Localization of the sensitive region was demonstrated with phantom samples by in vivo monitoring of rat livers and detection of necrotic regions of subcutaneously implanted tumors. The inversion-recovery pulse sequence, 2 theta-tau-theta(+/- x);[2 theta(+/- x, +/- y)]2, where tau is a variable delay, was employed to measure the spin-lattice relaxation time of a selected region, which could be varied by changing the pulse width and the size of the surface coil.

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Considerations for brain pH assessment by 31P NMR

Pettegrew

Withers

Panchalingam

et al. 1988

Magnetic Resonance Imaging

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31phosphate Nuclear Magnetic Resonance Spectrometry During Cerebral Hypoxia

Cited by 5 publications

References 0 publications

Brain intracellular pH and metabolism during hypercapnia and hypocapnia in the new‐born lamb.

Brain intracellular pH and metabolism during hypercapnia and hypocapnia in the new‐born lamb.

Depth pulse sequences for surface coils: spatial localization and T₁ measurements

Considerations for brain pH assessment by 31P NMR

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31phosphate Nuclear Magnetic Resonance Spectrometry During Cerebral Hypoxia

Cited by 5 publications

References 0 publications

Brain intracellular pH and metabolism during hypercapnia and hypocapnia in the new‐born lamb.

Brain intracellular pH and metabolism during hypercapnia and hypocapnia in the new‐born lamb.

Depth pulse sequences for surface coils: spatial localization and T1 measurements

Considerations for brain pH assessment by 31P NMR

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Depth pulse sequences for surface coils: spatial localization and T₁ measurements