(332) Anxiogenic-like behaviors induced by living with a conspecific in neuropathic pain condition are attenuated by systemic injection of midazolam in mice

Carmona, Isabela Miranda; Baptista-de-Souza, Daniela; Canto-de-Souza, Azair

doi:10.1016/j.jpain.2016.01.239

Cited by 7 publications

(10 citation statements)

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“…Previous findings by our laboratory have demonstrated that cohabitation with mice subjected to sciatic nerve constriction for 14 days induces anxiety behaviors in their cagemates assessed by the elevated plus maze test [6,12]. Therefore, our data indicate that the stress protocol described in this paper may be suitable for studying stress-induced emotional contagion.…”

Section: Discussionsupporting

confidence: 52%

“…For instance, studies have shown abnormal anxiety behaviors and pain sensitivity in rodents subjected to chronic sciatic nerve constriction (neuropathic pain model) [5,[7][8][9][10][11]. Likewise, studies conducted by our group demonstrated that the cagemates of animals experiencing chronic pain also exhibited anxiogenic-like behaviors on the elevated plus maze and hyperalgesia on the writhing test [6,12,13].…”

Section: Introductionmentioning

confidence: 84%

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Preliminary behavioral assessment of cagemates living with conspecifics submitted to chronic restraint stress in mice

Oliveira

Zaniboni

Carmona

et al. 2017

Neuroscience Letters

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The capacity of rodents to recognize and respond to emotional signs from a conspecific is a valuable adaptive behavior, which provides essential skills for species survival. However, repeated exposure to aversive situations may elicit maladaptive behavioral responses in subjects that experience noxious episodes and their colony members. Previous findings by our group demonstrated that living with a subject in neuropathic pain induces anxiogenic-like behaviors and hypernociception in mice. Whereas chronic pain may be considered a stressful stimulus, we extended our findings on stress-induced emotional transfer. For this purpose, we investigated whether cohabitation with a partner subjected to chronic restraint stress was able to promote alterations in anxiety-like behaviors, pain sensibility and defensive responses. Male Swiss mice were housed in pairs for 14days and then separated into control, stress, and cagemate groups. The stress group was subjected to 14days of restraint stress (1h/day) in the presence of the cagemates, while the pair-housed control group was left undisturbed. A day after last stress session control, stress, and cagemate groups were evaluated using elevated plus maze test, writhing test, and rat exposure test. Results demonstrated that chronic stress attenuated weight gain in the stress group. Moreover, cohabitation with mice subjected to chronic restraint stress induced anxiogenic-like behaviors, pain hypernociception, and alterations in defensive responses in both cagemate and stress groups. These preliminary findings suggest that chronic exposure to aversive stimulus may induce behavioral alterations even in observers.

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Section: Discussionsupporting

confidence: 52%

Section: Introductionmentioning

confidence: 84%

Preliminary behavioral assessment of cagemates living with conspecifics submitted to chronic restraint stress in mice

Oliveira

Zaniboni

Carmona

et al. 2017

Neuroscience Letters

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“…The present study investigated whether cohabitation with a conspecific in chronic pain condition would provoke changes in anxiety-like behavior in cagemates and whether systemic or intra-amygdala administration of midazolam would be able to reverse this effect of pain-induced emotional contagion. Confirming our previous results, we found that living, for 14 days, with mice submitted to sciatic nerve constriction induces anxiogenic-like behavior in cagemates tested in the EPM [15,17,18] and midazolam reversed this anxiogenic effect by both, systemic and intraamygdala administration. In addition to data showing midazolam effects on the EPM test, we complimented these previous findings showing increase in anxiety-like behaviors in mice subjected to neuropathic pain condition when compared to Sham group.…”

Section: Discussionsupporting

confidence: 90%

“…The emotional contagion induced by distress situation in a conspecific has been explored by our group using chronic sciatic nerve constriction [15,17,18] or chronic restraint stress [34]. These studies observed that cohabitation with a mouse submitted to chronic pain or chronic stress promotes decrease in exploration of open arms of the EPM.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, rodent models of emotional contagion employing a conspecific under physical harm might provide efficient evidences about emotional dysfunction observed in caregivers. Among some paradigms to investigate the effects pain-related emotional contagion [12][13][14], our research group adopted a preclinical approach which cagemates are co-housed with mice submitted to sciatic nerve injury [15][16][17][18][19][20]. Our previous findings demonstrated that observer/witness cagemates exhibit increased anxietylike behaviors in the EPM [15,17,18] and hyperalgesia in writhing test [15,16,19,20].…”

Section: Introductionmentioning

confidence: 99%

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Systemic and intra-amygdala administrations of midazolam reverse anxiety-like behavior induced by cohabiting with a cagemate in chronic pain condition

Carmona

Oliveira²,

Baptista-de-Souza³

et al. 2021

Preprint

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The affective component of pain may be shared among conspecifics through emotional contagion, a form of empathic expression. In this sense, reverberation of negative emotions could generate distress behavioral responses, such as pathological anxiety. Evidences reported that amygdala and its benzodiazepine receptors are involved in perception of pain in others. However, relatively little is known about the neural processes underlying emotional contagion induced by pain observation. In the present study, we investigated the effects of midazolam, an allosteric GABAergic receptor agonist, in anxiety-like behaviors induced by cohabitation with cagemate submitted to sciatic nerve constriction. For this purpose, we administrated systemic (0.5, 1.0 and 2.0 mg/kg) and intra-amygdala midazolam injections (3.0 and 30.0 nmol) in observer cagemates before elevated plus-maze (EPM) evaluation. We found that mice subjected to nerve constriction and their observer cagemates increased anxiety-like behavior in the EPM. Further, systemically (1.0 and 2.0 mg/kg) and intra-amygdala administration of midazolam (3.0 and 30 nmol) reverse this anxiogenic effect. Collectively, these results suggest that social interaction with a cagemate under chronic pain produces anxiety-like responses that could be blocked through midazolam application.

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Differential modulation of the anterior cingulate and insular cortices on anxiogenic-like responses induced by empathy for pain

et al. 2021

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(332) Anxiogenic-like behaviors induced by living with a conspecific in neuropathic pain condition are attenuated by systemic injection of midazolam in mice

Cited by 7 publications

References 0 publications

Preliminary behavioral assessment of cagemates living with conspecifics submitted to chronic restraint stress in mice

Preliminary behavioral assessment of cagemates living with conspecifics submitted to chronic restraint stress in mice

Systemic and intra-amygdala administrations of midazolam reverse anxiety-like behavior induced by cohabiting with a cagemate in chronic pain condition

Differential modulation of the anterior cingulate and insular cortices on anxiogenic-like responses induced by empathy for pain

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