3400 m/s deuterium pellet injector for Tore Supra

Perin, J.P.; Géraud, A.

doi:10.1016/b978-0-444-82220-8.50129-1

Cited by 8 publications

(7 citation statements)

References 1 publication

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“…Amino acids 14-39 of SytIV satisfy the criteria of the MOMENT program (26) for a transmembrane region but differ significantly from the transmembrane sequences of other synaptotagmins. Finally, the C-terminal 24 aa of SytIV share sequence similarities with the region of SytI responsible for binding to neurexin, a presynaptic membranespanning protein (27).…”

Section: Discussionmentioning

confidence: 99%

Synaptotagmin IV is an immediate early gene induced by depolarization in PC12 cells and in brain.

Vician

Lim

Ferguson

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

142

188

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Section: Discussionmentioning

confidence: 99%

Synaptotagmin IV is an immediate early gene induced by depolarization in PC12 cells and in brain.

Vician

Lim

Ferguson

et al. 1995

Proc. Natl. Acad. Sci. U.S.A.

142

188

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“…These two ligand-binding sites are not totally independent, however, because inositol 1,3,4,5-tetrakisphosphate binding to the C2B effector site strongly inhibited Ca 2ϩ -dependent self-oligomerization of the C2B domain in vitro (17) and Ca 2ϩ -binding mutations alter self-oligomerization activity (41). A third site, the conserved WHXL motif in the ␤8 strand, required for neurexin binding in vitro (10,42), is involved in the attachment of the Syt cytoplasmic domain with plasma membrane in PC12 cells (10,34), and is involved in synaptic vesicle docking to the active zones in the squid giant synapse (10). During the course of the affinity-purification of the present antibody, the ␤8 strand antibody was removed by passing through the column coupled with the C terminus of the C2B domain (see Materials and Methods for details).…”

Section: Discussionmentioning

confidence: 99%

Vesicular reuptake inhibition by a synaptotagmin I C2B domain antibody at the squid giant synapse

Llinás

Sugimori

Moran

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

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Synaptotagmin (Syt) I, a ubiquitous synaptic vesicle protein, comprises a transmembrane region and two C2 domains. The C2 domains, which have been shown to be essential for both synaptic vesicle exocytosis and endocytosis, are also seen as the Ca 2؉ sensors in synaptic vesicular release. In a previous study, we reported that a polyclonal antibody raised against the squid (Loligo pealei) Syt I C2B domain, while inhibiting vesicular endocytosis, was synaptic release neutral at the squid giant synapse. Recent reports concerning the C2B requirements for synaptic release prompted us to readdress the role of C2B in squid giant synapse function. Presynaptic injection of another anti-Syt I-C2B antibody (using recombinant whole C2B domain expressed in mammalian cell culture as an antigen) into the presynaptic terminal reproduced our previous results, i.e., reduction of vesicular endocytosis without affecting synaptic release. This set of results addresses the issue of the geometrical arrangement of the Ca 2؉ sensor, allowing the C2B domain antibody to restrict Ca 2؉ -dependent C2B selfoligomerization without modifying the Ca 2؉ -dependent release process.mollusk ͉ presynaptic ͉ synaptic transmission

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“…The first synaptic vesicle proteins we purified and cloned were synaptophysin,8 cytochrome b561,9 synapsins,10 synaptobrevins11 (also independently cloned by R.H. Scheller and named vesicle‐associated membrane protein [VAMP]12), proton pump components,13, 14 and synaptotagmins 15–17. In addition, we found that Rab3 proteins, the brain’s most abundant GTP‐binding proteins originally identified as ras‐homologous sequences,18 are associated with synaptic vesicles,19 and that Rab3 proteins cycle on and off synaptic vesicles during exocytosis 20…”

Section: Molecular Anatomy Of the Presynaptic Terminalmentioning

confidence: 99%

The Molecular Machinery of Neurotransmitter Release (Nobel Lecture)

2014

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The most important property of synaptic transmission is its speed, which is crucial for the overall workings of the brain. In his Nobel Lecture, T. C. Südhof explains how the synaptic vesicle and the plasma membrane undergo rapid fusion during neurotransmitter release and how this process is spatially organized, such that opening of Ca(2+) -channels allows rapid translation of the entering Ca(2+) signal into a fusion event.

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3400 m/s deuterium pellet injector for Tore Supra

Cited by 8 publications

References 1 publication

Synaptotagmin IV is an immediate early gene induced by depolarization in PC12 cells and in brain.

Synaptotagmin IV is an immediate early gene induced by depolarization in PC12 cells and in brain.

Vesicular reuptake inhibition by a synaptotagmin I C2B domain antibody at the squid giant synapse

The Molecular Machinery of Neurotransmitter Release (Nobel Lecture)

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