[39] Assay and partial purification of the cytokinin biosynthetic enzyme dimethylallylpyrophosphate: 5′-AMP transferase

Hommes, Norman G.; Akiyoshi, Donna E.; Morris, R. O.

doi:10.1016/s0076-6879(85)10092-3

Cited by 11 publications

(1 citation statement)

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“…Bacterial cytokinin biosynthetic activity was measured by using modifications of previously published methods (1,11 DNA isolation. Total DNA was isolated from Agrobacterium and Pseudomonas spp.…”

Section: Methodsmentioning

confidence: 99%

Cytokinin production by Agrobacterium and Pseudomonas spp

Akiyoshi¹,

Regier²,

Gordon³

1987

J Bacteriol

113

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The production of cytokinins by plant-associated bacteria was examined by radioimmunoassay. Strains producing trans-zeatin were identified in the genera Agrobacterium and Pseudomonas. Agrobacterium tumefaciens strains containing nopaline tumor-inducing plasmids, A. tumefaciens Lippia isolates, and Agrobacterium rhizogenes strains produced trans-zeatin in culture at 0.5 to 44 ,ug/liter. Pseudomonas solanacearum and Pseudomonas syringae pv. savastanoi produced trans-zeatin at levels of up to 1 mg/liter. In vitro cytokinin biosynthetic activity was measured for representative strains and was found to correlate with trans-zeatin production. The genetic locus for trans-zeatin secretion (tzs) was cloned from four strains: A. tumefaciens T37, A. rhizogenes A4, P. solanacearum K60, and P. syringae pv. savastanoi 1006. Southern blot analysis showed substantial homology of the Agrobacterium tzs genes to each other but not to the two Pseudomonas genes.In several plant-pathogen interactions, phytohormones have been shown to be involved with virulence (10,14,20,23,27 ture. One locus associated with indoleacetic acid production is located in the vir region and is required for tumorigenesis (17).Since cytokinin production has been previously reported in only a limited number of plant-associated bacteria, a survey was undertaken to determine if cytokinin production is a widespread phenomenon or if it is limited to only a few species. The identification of additional phytopathogens which synthesize tZ may be useful for future studies on the involvement of phytohormones in plant-pathogen interactions. MATERIALS AND METHODSBacterial strains and culture media. Bacterial strains used in this study are listed in Table 1. Bacteria were maintained on the following media: Escherichia coli, L agar (19); agrobacteria, AB minimal medium plus biotin (7); xanthomonas, AB minimal or King B medium (16); rhodococci, AB minimal or NBY (8 g of nutrient broth, 2 g of K2HPO4, 0.5 g of KH2PO4, 0.12 g of MgSO4, and 5 g of glucose per liter) medium; and P. syringae pathovars, King B medium. For short-term maintenance, P. solanacearum strains were maintained on CPG plates, but otherwise they were stored in water (15

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Section: Methodsmentioning

confidence: 99%