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Goulão, Luís F.; Valdiviesso, T.; Santana, Carlos; Oliveira, Cristina M.

doi:10.1023/a:1012053731052

Cited by 54 publications

(8 citation statements)

References 21 publications

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“…Although several molecular marker systems are currently available in the literature, most of the studies so far performed have employed only one marker system for the analysis and characterization of cultivated chestnuts. Recently, comparison between 2 marker methods on genetic diversity in chestnut is becoming common (Goulão et al, 2001;Martin et al, 2010). The dominant markers (RAPD, AFLP, and ISSR) used in this study provide a large number of polymorphic loci and were in general agreement with other studies of genetic diversity measurements.…”

Section: Discussionsupporting

confidence: 78%

“…So far, however, only a few studies have investigated the genetic diversity of cultivars using multiple markers on the same chestnut population materials simultaneously (Goulão et al, 2001;Martin et al, 2010). Accordingly, the present study was undertaken to apply 4 DNA markers [random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), intersimple sequence repeat (ISSR), and simple sequence repeat (SSR)] to detect the genetic variability among and within Castanea species and to compare their effectiveness and utility in estimating the genetic variation among chestnut cultivars.…”

Section: Introductionmentioning

confidence: 99%

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The assessment of genetic diversity of Castanea species by RAPD, AFLP, ISSR, and SSR markers

Abdelhamid¹,

Le²,

Conedera³

et al. 2014

Turk J Bot

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Castanea Mill. (chestnut) is a multipurpose deciduous tree and member of the family Fagaceae, widely distributed throughout North America, Europe, and Asia. The evaluation of the genetic diversity of chestnut species is crucial for the effective conservation of this economically and ecologically valuable tree. In this study, we applied 4 DNA markers to detect the genetic variability among and within Castanea species and to compare the effectiveness of each system in estimating genetic variation. We amplified 106 random amplified polymorphic DNA (RAPD), 228 amplified fragment length polymorphism (AFLP), 42 intersimple sequence repeat (ISSR), and 36 simple sequence repeat (SSR) polymorphic markers using 12, 5, 4, and 5 primer combinations, respectively. The findings on the effective multiplex ratio, polymorphism information content, and marker index revealed that AFLP was the most effective molecular marker system used in this study. Each marker system classified the species under investigation into clear but incompletely separated clusters, although partial agreement was achieved with respect to species relationships when the RAPD method was employed. The comparison of the correlation coefficient of RAPD marker data and the other markers showed a higher correlation [(r = 0.69, P < 0.01), (r = 0.77, P < 0.01), and (r = 0.47, P < 0.01) with AFLP, ISSR, and SSR, respectively]. When variance was partitioned among and within groups, AFLP (94.62%) showed greater variation within the groups and reverse RAPD (67.87%) yielded greater variation among the groups. Overall, the results indicate that the AFLP represents an efficient molecular marker system for the assessment of chestnut genetic diversity and, hence, the development of effective conservation strategies to preserve this valuable tree species.

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Section: Discussionsupporting

confidence: 78%

Section: Introductionmentioning

confidence: 99%

The assessment of genetic diversity of Castanea species by RAPD, AFLP, ISSR, and SSR markers

Abdelhamid¹,

Le²,

Conedera³

et al. 2014

Turk J Bot

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“…SSR molecular markers of genome polymorphism have increasingly been used to analyze genetic relationships (Luı´s, Teresa, Carlos, & Cristina, 2001) and genetic stability (Martins, Sarmento, & Oliveira, 2004). The aim of this work is to characterize nuts from ''Judia'' variety, collected in different climatic localities, trying to find the optimal temperature sum conditions regarding the evaluation of the quality and fruit size.…”

Section: Introductionmentioning

confidence: 99%

Study of morphological and chemical diversity in chestnut trees (var. “Judia”) as a function of temperature sum Estudio de la diversidad morfológica y química del fruto de castaña (var. “Judia”) en función de la suma de la temperatura

Dinis

Ferreira‐Cardoso

Peixoto

et al. 2011

CyTA - Journal of Food

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"Judia") en función de la suma de la temperatura, CyTA -Journal of Food, 9:3, 192-199, DOI: 10.1080/19476337.2010 Resultados contrarios a e´stos fueron los obtenidos para los frutos de la localidad ma´s ca´lida (Murc¸a: 2751 8D), que fueron los que registraron taman˜os ma´s pequen˜os (157,14 frutos/kg). En 2007, los mayores frutos procedieron de Macedo Cavaleiros (2163 8D), 46,4 frutos/kg, siendo de nuevo Murc¸a (2338 8D) la que proporciono´los frutos ma´s pequen˜os (66,0 frutos/kg). Las distancias gene´ticas de Nei entre los a´rboles oscilaron entre 0,0 y 0227, demostrando que todos ellos son de la misma variedad.

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“…The first markers used were isozymes (Sawano et al 1984;Müller-Starck et al 1994), followed by random amplified polymorphic DNAs (RAPDs) (Fineschi et al 1994). Some more recent studies employed microsatellite (SSR) markers (Botta et al 1999(Botta et al , 2001Buck et al 2003;Marinoni et al 2003) and inter simple sequence repeats (ISSRs) (Goulão et al 2001). The need for new, reliable SSR markers is always increasing, not only for cultivar identification but also for more accurate genome mapping (Casasoli et al 2001;Barreneche et al 2004) and QTL analysis.…”

Section: Introductionmentioning

confidence: 99%

Microsatellite-based characterization of the Castanea sativa cultivar heritage of southern Switzerland

Gobbin

Hohl

Conza

et al. 2007

Genome

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Southern Switzerland has a long tradition of chestnut cultivation as a staple food. Local inhabitants constantly selected varieties according to the ripening period, the type of use, and the adaptability to the territory. As a result, the panorama of chestnut varieties is very complex, as reflected by more than 120 different variety names in an area of 26,000 ha. Since 1994, 47 varieties have been conserved in the chestnut germplasm of southern Switzerland (CSS), including Marroni, Euro-Japanese, and French varieties. A selection of 164 individuals from the CSS was analysed by 8 SSR markers (4 of which were developed in this study). Microsatellite analysis indicated that the CSS was accurately established, as 86% of the individuals grafted were correctly labeled. The identification of 98 genotypes, 10 clonal chestnut groups, 4 synonym groups, and 12 homonym groups reflected the complex ethnogeographical structure of the chestnut distribution. The 17 Marroni individuals considered clustered in 2 differentiated genetic groups instead of only 1 as expected. The fundamental problem of the frequent cases of homonymy and synonymy is discussed, as is the need for criteria for discriminating between polyclonal varieties and distinct homonymous varieties.

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Cited by 54 publications

References 21 publications

The assessment of genetic diversity of Castanea species by RAPD, AFLP, ISSR, and SSR markers

The assessment of genetic diversity of Castanea species by RAPD, AFLP, ISSR, and SSR markers

Study of morphological and chemical diversity in chestnut trees (var. “Judia”) as a function of temperature sum Estudio de la diversidad morfológica y química del fruto de castaña (var. “Judia”) en función de la suma de la temperatura

Microsatellite-based characterization of the Castanea sativa cultivar heritage of southern Switzerland

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