This chapter presents a broad overview of the principles, practice and some of the new developments in aquatic toxicology. The field has matured rapidly over the past 30‐40 years. Many acute and chronic toxicity testing methods have been developed and standardized for application in environmental protection. Basic research has been conducted with many species across the aquatic phyla, with a wide range of contaminants. Critical linkages have been made to other aquatic disciplines ensuring that a comprehensive understanding of exposure, uptake, metabolism and discharge of chemicals, in many aquatic species under many conditions, is fully realized. New issues, such as the effects of pharmaceuticals, are being investigated with urgency. Aquatic toxicology is now supported in many countries by a wide range of research programmes, graduate programmes, training courses and publications, assuring its place in basic and applied toxicology.