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Olivier, Jean-Christophe; Fénart, Laurence; Chauvet, Romain; Pariat, Claudine; Cecchelli, Roméo; Couet, William

doi:10.1023/a:1018947208597

Cited by 195 publications

(42 citation statements)

References 13 publications

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“…While some groups have reported that nanoparticles can exert cytotoxic effects in bovine brain microvessel endothelial cells (42,43), Lockman et al (44) demonstrated that in the presence of emulsifying wax SLNs, no overall significant differences were observed in vivo for the cerebral perfusion flow. Furthermore, these authors reported that no statistically significant changes in barrier integrity, membrane permeability, or facilitated choline transport occurred in vitro in bovine brain microvessel endothelial cells.…”

Section: Discussionmentioning

confidence: 99%

Solid Lipid Nanoparticles Enhance the Delivery of the HIV Protease Inhibitor, Atazanavir, by a Human Brain Endothelial Cell Line

et al. 2008

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Section: Discussionmentioning

confidence: 99%

Solid Lipid Nanoparticles Enhance the Delivery of the HIV Protease Inhibitor, Atazanavir, by a Human Brain Endothelial Cell Line

et al. 2008

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“…Dalargin HPLC Analysis. After separation of free dalargin from nanoparticle suspension, samples were analyzed for dalargin concentration by a HPLC method modified from that of Olivier et al 19 using an Agilent 1100 HPLC apparatus (Agilent Technologies, USA). Briefly, an isocratic reversed-phase HPLC with a Symmetry C18 5 lm column (150 3 4.6 mm 2 ) was used.…”

Section: Methodsmentioning

confidence: 99%

Effect of formulation factors on incorporation of the hydrophilic peptide dalargin into PLGA and mPEG‐PLGA nanoparticles

2008

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The objective of this study was to examine formulation factors that influence the incorporation of the hydrophilic peptide dalargin into poly(D, L-lactic-co-glycolic acid) (PLGA) and methoxy-polyethylene glycol (mPEG)-PLGA nanoparticles. In particular, the effect of ionic additives and nanoparticle method of preparation on the incorporation of dalargin and resultant nanoparticle properties was investigated. Biodegradable nanoparticles were prepared from mPEG-PLGA and PLGA by both solvent evaporation and solvent diffusion methods with inclusion of ionic additives of dextran sulphate (DS), sulfobutyl ether-beta-cyclodextrin (SB-CD), or sodium dodecyl sulfate (SDS). The resultant nanoparticles were analyzed for their mean particle size and size distribution, zeta-potential, peptide loading, yield, and morphology. The inclusion of ionic additives in the nanoparticle formulation significantly influenced dalargin entrapment efficiency (EE). For example, with the PLGA/SDS formulation EE increased from 13.3% to 91.2% and from 4.1% to 68.6% with the solvent diffusion and evaporation methods, respectively. The inclusion of ionic surfactant SDS has also lead to the formation of smaller size of nanoparticles. Isothermal titration microcalorimetry revealed a strong interaction between dalargin and DS, medium level interaction with SDS, and weak interaction with SB-CD. The results of this study suggest that a strong ionic interaction between peptides and additives may lead to enhanced peptide incorporation but also increased particle size. Intermediate ionic interaction, especially when it is associated with the formation of reversed micelles in a hydrophobic polymer solution, could be used to enhance the incorporation of hydrophilic peptides in PLGA and mPEG-PLGA nanoparticles.

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“…A reported high performance liquid chromatographic (HPLC) method 22 was adopted for analysis of dalargin in entrapment and release studies. HPLC analysis was carried out using the Agilent 1100 instrument (Agilent technologies, USA) with a multi-diodearray detector.…”

Section: Hplc Analysis Of Dalarginmentioning

confidence: 99%

Development of a chitosan‐based nanoparticle formulation for delivery of a hydrophilic hexapeptide, dalargin

et al. 2008

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Nanoparticle based delivery systems can offer opportunities for targeting, controlled release, and enhanced stability of their drug, protein, or gene therapy payload. This study investigated the use of chitosan in combination with the ionic additives sulfobutyl-ether-7-beta-cyclodextrin (SB-CD) or SB-CD/dextran sulfate (SB-CD/DS) mixture in comparison with chitosan: DS in the formulation of nanoparticles incorporating the hexapeptide dalargin. The physical characteristics (particle size, zeta potential), entrapment and loading efficiency, and release of dalargin were quantified. It was demonstrated that anionic cyclodextrin, SB-CD, can be used in complex coacervation with chitosan, with and without the presence of DS, to form nanoparticles. The presence of SB-CD or DS in the nanoparticle formulation and the weight ratio of chitosan to anionic additive(s) influenced the physical properties of the nanoparticles and their ability to carry dalargin. In addition, the particle size of nanoparticles was also affected by the molecular weight of chitosan and DS. The use of either DS or SB-CD/DS mixture produced chitosan nanoparticles with small particle size, high dalargin entrapment efficiency, enhanced peptide stability, and sustained release characteristics.

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Abstract: A non specific permeabilization of the BBB, probably related to the toxicity of the carrier, may account for the CNS penetration of dalargin associated with PBCA NP and polysorbate 80.

Cited by 195 publications

References 13 publications

Solid Lipid Nanoparticles Enhance the Delivery of the HIV Protease Inhibitor, Atazanavir, by a Human Brain Endothelial Cell Line

Solid Lipid Nanoparticles Enhance the Delivery of the HIV Protease Inhibitor, Atazanavir, by a Human Brain Endothelial Cell Line

Effect of formulation factors on incorporation of the hydrophilic peptide dalargin into PLGA and mPEG‐PLGA nanoparticles

Development of a chitosan‐based nanoparticle formulation for delivery of a hydrophilic hexapeptide, dalargin

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