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Çi̇ftçi, Yılmaz; Eroğlu, Oğuzhan; Firidin, Şirin

doi:10.4194/1303-2712-v13_2_11

Cited by 13 publications

(5 citation statements)

References 51 publications

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“…The D-Loop was amplified on a subsample of 59 individuals (Supplementary Material S1) using the primer pair ProF-PheR [32], whilst the cyt b with the primer pair Cyt-f-Cyt-r [14] was analyzed on a smaller subsample of 14 individuals (Supplementary Material S1). PCR reactions were performed using Multiplex PCR Master Mix (QIAGEN) according to the manufacturer's instructions, using about 20 ng of DNA and 0.25 µL of each primer pair in 10 µL total volume reaction.…”

Section: Molecular Methodsmentioning

confidence: 99%

“…Due to the long lifecycle and anadromous status, sturgeons are very susceptible to over-exploitation, pollution, and especially to habitat fragmentation [13]; among sturgeons, beluga sturgeon is known to undertake the longest upstream migration. Consequently, dams and embankments have strongly impacted this species [14], even though river fragmentation is not the only driving cause of H. huso decline [13]. Beluga sturgeon is, thus, listed as critically endangered under the International Union for Conservation of Nature (IUCN) World Red Data Book [15]; it is also included in appendixes II and III of the Bern Convention (CE 1979) and in the annex V of the European Habitat Directive 92/43/CEE [16].…”

Section: Introductionmentioning

confidence: 99%

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Genetic Investigation of Four Beluga Sturgeon (Huso huso, L.) Broodstocks for its Reintroduction in the Po River Basin

et al. 2021

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The reintroduction of the extinct beluga sturgeon (Huso huso L.), an anadromous species with economic and traditional relevance, is a priority in next conservation strategies in Northern Italy. The EU-LIFE NATURA project aims to reintroduce the beluga sturgeon in the Po River basin through a captive breeding program. Critical requirements for the success of the program are river connectivity and knowledge of genetic diversity of the selected broodstocks to ensure self-sustainability of reintroduced populations. Here, the four broodstocks used for the reintroduction of beluga sturgeon have been genetically screened, genotyping 13 loci and sequencing mitochondrial DNA cytochrome b (Cyt b) gene and the entire mitochondrial DNA control region (D-Loop). The four broodstocks showed a medium-high level of nuclear genetic variability and the presence of two sub-populations, evidencing a total level of inbreeding coefficients able to sustain the good potential as future breeders. Mitochondrial analyses showed a genetic variability comparable to wild populations, further strengthening the positive potential of the investigated broodstock. Therefore, this study, showed how the degree of genetic diversity found within the four broodstocks used for H. huso reintroduction in the Po River basin could be suitable to ensure the success of the program, avoiding the inbreeding depression associated with founder effect and captive breeding.

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Section: Molecular Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genetic Investigation of Four Beluga Sturgeon (Huso huso, L.) Broodstocks for its Reintroduction in the Po River Basin

et al. 2021

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“…Two mitochondrial markers (cytochrome b gene and D-loop region) were used to assess the genetic diversity of stellate sturgeon. The cytochrome b (cyt b) gene was fully amplified (1141 bp, complete CDS) using GoTaq ® Flexi DNA Polymerase (Promega, USA) reagents and a specific primer set and protocol previously described by Çiftci, Ero glu & Firidin (2013). The D-loop region was partially amplified (730 bp) using GoTaq ® Flexi DNA Polymerase (Promega, USA) reagents and a specific primer set and protocol previously described by Congiu et al (2011).…”

Section: Mtdna Amplification and Sequencingmentioning

confidence: 99%

Genetic population structure of the critically endangered stellate sturgeon (Acipenser stellatus) in the Black Sea basin: Implications for conservation

Holostenco

Ciorpac

Firidin

et al. 2022

Aquatic Conservation

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1. Stellate sturgeon (Acipenser stellatus) is a species of great conservation concern throughout its range. Over the past century, it has experienced a dramatic decline in abundance and distribution in the Black Sea basin. Information regarding the genetic structure of the species is very limited in the region, despite its crucial importance for developing adequate management plans for the species' conservation and recovery.2. Samples from 163 A. stellatus were analysed using mitochondrial and microsatellite DNA markers to investigate the species' genetic characteristics in the north-western and southern Black Sea. 3. Both genetic markers revealed high genetic diversity (153 haplotypes and 264 alleles) and low genetic differentiation of marine and freshwater catches. The north-western Black Sea population is undergoing a slight demographic expansion after a bottleneck event induced by overfishing and dam constructions. 4. The genetic population structure indicates a single genetic cluster in the northwestern Black Sea region and signs of admixture of two genetic clusters in the southern region. It also provides the first evidence for a remnant reproductive stellate sturgeon micro-population in rivers of Turkey and Georgia. 5. A sturgeon fishing ban and stocking activities carried out during the last decades, largely without any prior genetic assessment of the sturgeon populations, added little conservation value. Outreach initiatives and urgent actions are required to foster the recovery of this Critically Endangered species in the region. Daniela Nicoleta Holostenco and Mitic a Ciorpac should be considered joint first authors.Geta Rîs ¸noveanu and Klaus Kohlmann should be considered joint senior authors.

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“…The COI and CytB have moderate evolutionary rates than other mtDNA genes (Kochzius et al, 2010;Kartavtsev, 2011) and their fragments contain adequate informative phylogenetic information (Liu et al, 2021). The coupled use of COI and CytB had not only been applied to barcode animal taxa and infer deep intraspecies divergences (Baker et al, 1995;Smith et al, 2011;Çiftçi et al, 2013;Joshi et al, 2019), but also to detect provisional cryptic populations awaiting thorough screenings (Asgharian et al, 2011;Hubert et al, 2012;Sienes et al, 2014;Thongtam Na Ayudhaya et al, 2017). Findings from this study serve as baseline information for the fishery management and sustainable utilization of S. leptolepis given its economic relevance and understudied status.…”

Section: Introductionmentioning

confidence: 99%

First Evidence of Cryptic Species Diversity and Population Structuring of Selaroides leptolepis in the Tropical Western Pacific

2021

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The yellowstripe scad, Selaroides leptolepis (Carangidae), is an important fish commodity in the Tropical Western Pacific (TWP). It has a latitudinal Pacific range from south of Japan down to northern Australia, with the highest concentration in Southeast Asia. However, its TWP fishing grounds have long been a hotspot of unsustainable exploitations, thus threatening the remaining wild populations. Despite the species’ commercial significance, there is limited understanding of its genetic structure and diversity. Herein, the genetic structure of S. leptolepis was examined using mitochondrial COI and CytB sequences. Both markers denoted significant genetic structuring based on high overall FST values. Hierarchical analysis of molecular variance (AMOVA), maximum likelihood (ML) phylogenetic trees, and median-joining (MJ) haplotype networks strongly supported the occurrence of two allopatrically distributed lineages. These comprised of a widespread Asian lineage and an isolated Australian lineage. Within-lineage distances were low (K2P < 1%) whereas across-lineage distances were remarkably high (K2P > 6%), already comparable to that of interspecific carangid divergences. Haplotype sequence memberships, high genetic variations, and the geographic correlation suggested that the Australian lineage was a putative cryptic species. Historical demographic inferences also revealed that the species experienced rapid expansion commencing on the late Pleistocene, most likely during the end of the Last Glacial Maximum (∼20,000 years ago). The present study encouraged the application of lineage-specific management efforts, as the lineages are experiencing different evolutionary pressures. Overall, accurate knowledge of the species’ genetic distribution is fundamental in protecting its diversity and assuring stock sustainability.

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Cited by 13 publications

References 51 publications

Genetic Investigation of Four Beluga Sturgeon (Huso huso, L.) Broodstocks for its Reintroduction in the Po River Basin

Genetic Investigation of Four Beluga Sturgeon (Huso huso, L.) Broodstocks for its Reintroduction in the Po River Basin

Genetic population structure of the critically endangered stellate sturgeon (Acipenser stellatus) in the Black Sea basin: Implications for conservation

First Evidence of Cryptic Species Diversity and Population Structuring of Selaroides leptolepis in the Tropical Western Pacific

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