3D angioarchitecture changes after spinal cord injury in rats using synchrotron radiation phase-contrast tomography

Hu, Jianzhong; Cao, Yuze; Wu, Tianding; Li, D; Lü, Hongbin

doi:10.1038/sc.2015.49

Cited by 15 publications

(9 citation statements)

References 31 publications

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“…Among all these imaging methods, PPCI is the simplest to implement, requiring only a double-crystal monochromator without the need for additional complex optical components 21 22 . PPCI can provide excellent phase contrast information about soft tissue, such as the liver, brain and spinal cord vessels, and cartilage, especially in the human knee joints 23 24 25 26 27 28 29 . The 3D morphology of small objects, even individual cells, can be obtained with high spatial and contrast resolution using this method 30 .…”

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confidence: 99%

3D visualization of the lumbar facet joint after degeneration using propagation phase contrast micro-tomography

Cao

Zhang

Yin

et al. 2016

Sci Rep

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Lumbar facet joint (LFJ) degeneration is believed to be an important cause of low back pain (LBP). Identifying the morphological changes of the LFJ in the degeneration process at a high-resolution level could be meaningful for our better understanding of the possible mechanisms underlying this process. In the present study, we determined the 3D morphology of the LFJ using propagation phase contrast micro-tomography (PPCT) in rats to assess the subtle changes that occur during the degeneration process. PPCT provides vivid 3D images of micromorphological changes in the LFJ during its degeneration process, and the changes in the subchondral bone occurred earlier than in the cartilage during the early stage of degeneration of the LFJ. The delineation of this alteration was similar to that with the histological method. Our findings demonstrated that PPCT could serve as a valuable tool for 3D visualization of the morphology of the LFJ by providing comprehensive information about the cartilage and the underlying subchondral bone and their changes during degeneration processes. It might also have great potential for providing effective diagnostic tools to track changes in the cartilage and to evaluate the effects of therapeutic interventions for LFJ degeneration in preclinical studies.

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mentioning

confidence: 99%

3D visualization of the lumbar facet joint after degeneration using propagation phase contrast micro-tomography

Cao

Zhang

Yin

et al. 2016

Sci Rep

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“…In addition to inhibiting astrogliosis, it has been reported that hypothermia can promote the expression of pro-angiogenic factors. Vascular disruption is a common traumatic event after SCI, resulting in hemorrhage, a reduction in spinal cord blood flow and ischemia [ 106 , 107 , 108 ]. Accordingly, inducing angiogenesis is a therapeutic strategy to promote spinal cord repair.…”

Section: Mechanisms Of Hypothermia-mediated Protectionmentioning

confidence: 99%

Therapeutic Hypothermia in Spinal Cord Injury: The Status of Its Use and Open Questions

Wang

Pearse

2015

IJMS

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Spinal cord injury (SCI) is a major health problem and is associated with a diversity of neurological symptoms. Pathophysiologically, dysfunction after SCI results from the culmination of tissue damage produced both by the primary insult and a range of secondary injury mechanisms. The application of hypothermia has been demonstrated to be neuroprotective after SCI in both experimental and human studies. The myriad of protective mechanisms of hypothermia include the slowing down of metabolism, decreasing free radical generation, inhibiting excitotoxicity and apoptosis, ameliorating inflammation, preserving the blood spinal cord barrier, inhibiting astrogliosis, promoting angiogenesis, as well as decreasing axonal damage and encouraging neurogenesis. Hypothermia has also been combined with other interventions, such as antioxidants, anesthetics, alkalinization and cell transplantation for additional benefit. Although a large body of work has reported on the effectiveness of hypothermia as a neuroprotective approach after SCI and its application has been translated to the clinic, a number of questions still remain regarding its use, including the identification of hypothermia’s therapeutic window, optimal duration and the most appropriate rewarming rate. In addition, it is necessary to investigate the neuroprotective effect of combining therapeutic hypothermia with other treatment strategies for putative synergies, particularly those involving neurorepair.

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“…Within SRμCT studies, the number of collected projections reported can vary widely. Various nervous system studies have collected 720 20 , 21 , 501–2000 22 , 1600 2 , 1800–2400 23 and 2000–2400 3 projections. The minimal number of projections that should be collected to avoid undersampling at the edge of the field of view is calculated by multiplying the pixel width of an imaging system (perpendicular to the rotation axis) by π/2 19 .…”

Section: Introductionmentioning

confidence: 99%

“…Inline phase contrast is a simple phase imaging method in which contrast is increased by increasing the propagation distance (PD) between sample and detector (or scintillator, for a scintillator-coupled camera). It is unclear whether phase contrast can augment tissue stain contrast, but inline phase contrast of unstained samples has enabled visualisation of murine spinal vasculature 20 , large-diameter motoneurons 22 , 29 , cerebral vasculature 5 and individual Purkinje cells in human post-mortem cortical tissue 3 . Details of how phase contrast was optimised have not commonly been reported, with PDs for nervous system samples varying from 300 mm 20 to 500 mm 5 and even 2,300 mm 22 .…”

Section: Introductionmentioning

confidence: 99%

“…It is unclear whether phase contrast can augment tissue stain contrast, but inline phase contrast of unstained samples has enabled visualisation of murine spinal vasculature 20 , large-diameter motoneurons 22 , 29 , cerebral vasculature 5 and individual Purkinje cells in human post-mortem cortical tissue 3 . Details of how phase contrast was optimised have not commonly been reported, with PDs for nervous system samples varying from 300 mm 20 to 500 mm 5 and even 2,300 mm 22 . Determining the optimal PD is non-trivial, because if it is too small, contrast improvements will be insufficient to detect features of interest, but if it is too large, Fresnel fringes will grow to obscure a sample’s true profile, leading to inaccurate data.…”

Section: Introductionmentioning

confidence: 99%

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Optimising complementary soft tissue synchrotron X-ray microtomography for reversibly-stained central nervous system samples

Strotton

Bodey

Wanelik

et al. 2018

Sci Rep

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Synchrotron radiation microtomography (SRμCT) is a nominally non-destructive 3D imaging technique which can visualise the internal structures of whole soft tissues. As a multi-stage technique, the cumulative benefits of optimising sample preparation, scanning parameters and signal processing can improve SRμCT imaging efficiency, image quality, accuracy and ultimately, data utility. By evaluating different sample preparations (embedding media, tissue stains), imaging (projection number, propagation distance) and reconstruction (artefact correction, phase retrieval) parameters, a novel methodology (combining reversible iodine stain, wax embedding and inline phase contrast) was optimised for fast (~12 minutes), high-resolution (3.2–4.8 μm diameter capillaries resolved) imaging of the full diameter of a 3.5 mm length of rat spinal cord. White-grey matter macro-features and micro-features such as motoneurons and capillary-level vasculature could then be completely segmented from the imaged volume for analysis through the shallow machine learning SuRVoS Workbench. Imaged spinal cord tissue was preserved for subsequent histology, establishing a complementary SRμCT methodology that can be applied to study spinal cord pathologies or other nervous system tissues such as ganglia, nerves and brain. Further, our ‘single-scan iterative downsampling’ approach and side-by-side comparisons of mounting options, sample stains and phase contrast parameters should inform efficient, effective future soft tissue SRμCT experiment design.

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3D angioarchitecture changes after spinal cord injury in rats using synchrotron radiation phase-contrast tomography

Cited by 15 publications

References 31 publications

3D visualization of the lumbar facet joint after degeneration using propagation phase contrast micro-tomography

3D visualization of the lumbar facet joint after degeneration using propagation phase contrast micro-tomography

Therapeutic Hypothermia in Spinal Cord Injury: The Status of Its Use and Open Questions

Optimising complementary soft tissue synchrotron X-ray microtomography for reversibly-stained central nervous system samples

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