3D-bioprinted peptide coupling patches for wound healing

Abstract

Chronic wounds caused by severe trauma remain a serious challenge for clinical treatment. In this study, we developed a novel angiogenic 3D-bioprinted peptide patch to improve skin wound healing. The 3D-bioprinted technology can fabricate individual patches according to the shape characteristics of the damaged tissue. Gelatin methacryloyl (GelMA) and hyaluronic acid methacryloyl (HAMA) have excellent biocompatibility and biodegradability, and were used as a biomaterial to produce bioprinted patches. The pro-angiogenic QHREDGS peptide was covalently conjugated to the 3D-bioprinted GelMA/HAMA patches, extending the release of QHREDGS and improving the angiogenic properties of the patch. Our results demonstrated that these 3D-bioprinted peptide patches showed excellent biocompatibility, angiogenesis, and tissue repair both in vivo and in vitro . These findings indicated that 3D-bioprinted peptide patches improved skin wound healing and could be used in other tissue engineering applications.

In-situ formed elastin-based hydrogels enhance wound healing via promoting innate immune cells recruitment and angiogenesis

“…GAE-50 group showed consistent outstanding proangiogenic effect in vitro and in vivo, which may be due to its good biostability. The formation of new blood capillary is known as a key characteristic in the proliferation stage, which promises the transport of adequate cells, nutrients and oxygen to the injured tissues [ 52 ]. Accelerated angiogenesis can help to promote the synthesis of collagen fiber in the wound site.…”

Wound-Microenvironment Engineering through Advanced-Dressing Bioprinting

“…We evidenced the immaturity of bioink developments (TRL 3–4) in contrast to the rapid advancements of bioprinter devices (TRL 9) [ 25 ]. However, in the last two years, there has been an expansion of bioink research for wound healing and novel blends have been proposed [ 16 , 26 , 27 , 28 ]. Adding to bioink advancements for extrusion bioprinting, we blended two natural hydrogels, sharing a common crosslinking mechanism for the post-printing stability of the construct.…”

Innovative Treatment Strategies to Accelerate Wound Healing: Trajectory and Recent Advancements

“…Herein, biocompatible biopolymers gelatin methacryloyl (GelMA) and hyaluronic acid methacryloyl (HAMA) that were covalently conjugated with QHREDGS peptide were utilized for bioprinting. The bioprinted peptide-containing patch demonstrated improved angiogenesis, biocompatibility, and wound repair both in in vitro and in vivo conditions [ 233 ]. In another study, a bioprinted tissue co-culture platform using a mixture of plasma-derived fibrinogen-containing factor XIII, fibronectin, thrombin, and macrophages (an FPM “bio-ink”) was utilized for stimulated wound closure and re-epithelialization in murine dermal wound model through extrusion bioprinting [ 226 ].…”