3D bioprinting for fabricating artificial skin tissue

Gao, Chuang; Lü, Chunxiang; Jian, Zhian; Zhang, Tingrui; Chen, Zhongjian; Zhu, Quangang; Tai, Zongguang; Liu, Yuanyuan

doi:10.1016/j.colsurfb.2021.112041

Cited by 62 publications

(46 citation statements)

References 112 publications

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“…Engineered skin not only provides advanced constructs to better replicate human skin, but also agrees with policies that tend to reduce the use of laboratory animals as in vivo models [251,252]. In agreement with 3R principles (replacement, reduction, and refinement) defined by Russell and Burch in 1959 for animal use in research [253], the current regulations and ethical concerns on animal testing and the need of skin substitutes with more physiological functions explain the significant advances in the development of 3D, innovative skin models in the last years [254]. For example, at present, the European Union has decreed the prohibition of the use of animals for testing of cosmetic ingredients.…”

Section: The 3d Bioprinted Alternative Skin Modelssupporting

confidence: 73%

The 3D Bioprinted Scaffolds for Wound Healing

et al. 2022

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Skin tissue engineering and regeneration aim at repairing defective skin injuries and progress in wound healing. Until now, even though several developments are made in this field, it is still challenging to face the complexity of the tissue with current methods of fabrication. In this review, short, state-of-the-art on developments made in skin tissue engineering using 3D bioprinting as a new tool are described. The current bioprinting methods and a summary of bioink formulations, parameters, and properties are discussed. Finally, a representative number of examples and advances made in the field together with limitations and future needs are provided.

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Section: The 3d Bioprinted Alternative Skin Modelssupporting

confidence: 73%

The 3D Bioprinted Scaffolds for Wound Healing

et al. 2022

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“…Typically, the 3D bioprinting process of human skin tissue can be summarized as the following three steps: 1) cell selection and cultivation, bio-ink selection and preparation, and 3D model design of skin; 2) the actual printing process and maturation of the printed skin constructs; and 3) histological evaluation and biochemical characterization of the printed skin tissue [ 30 ]. At present, extrusion-based bioprinting, inkjet bioprinting, and laser-assisted bioprinting are the most commonly used bioprinting techniques for the fabrication of skin tissue [ 24 , 29 ].…”

Section: Discussionmentioning

confidence: 99%

Simple and robust 3D bioprinting of full-thickness human skin tissue

et al. 2022

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Artificial skins have been used as skin substitutes for wound healing in the clinic, and as in vitro models for safety assessment in cosmetic and pharmaceutical industries. The three-dimensional (3D) bioprinting technique provides a promising strategy in the fabrication of artificial skins. Despite the technological advances, many challenges remain to be conquered, such as the complicated preparation conditions for bio-printed skin and the unavailability of stability and robustness of skin bioprinting. Here, we formulated a novel bio-ink composed of gelatin, sodium alginate and fibrinogen. By optimizing the ratio of components in the bio-ink, the design of the 3D model and the printing conditions, a fibroblasts-containing dermal layer construct was firstly fabricated, on the top of which laminin and keratinocytes were sequentially placed. Through air-liquid interface (ALI) culture by virtue of sterile wire mesh, a full-thickness skin tissue was thus prepared. HE and immunofluorescence staining showed that the bio-printed skin was not only morphologically representative of the human skin, but also expressed the specific markers related to epidermal differentiation and stratum corneum formation. The presented easy and robust preparation of full-thickness skin constructs provides a powerful tool for the establishment of artificial skins, holding critical academic significance and application value.

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“…Cells and scaffolds are generally made of polymers and extruded from a nozzle and cultured in 3D ( Elalouf, 2021 ; Jamee et al, 2021 ). Many organs, such as bone ( Arrigoni et al, 2017 ; Kim et al, 2021 ; Alcala-Orozco et al, 2022 ), lung ( Hu et al, 2021 ), and skin ( Gao et al, 2021c ), have already been generated by 3D bioprinting, showing plenty of potential in the future. Cell delivery ( Hwang et al, 2022 ) and tumor modeling ( Samadian et al, 2021 ) can also be achieved by bioprinting.…”

Section: Advanced Models For Ex Vivo Non Studiesmentioning

confidence: 99%

Lab-on-Chip Microsystems for Ex Vivo Network of Neurons Studies: A Review

Zhang

Rong

Bian

2022

Front. Bioeng. Biotechnol.

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Increasing population is suffering from neurological disorders nowadays, with no effective therapy available to treat them. Explicit knowledge of network of neurons (NoN) in the human brain is key to understanding the pathology of neurological diseases. Research in NoN developed slower than expected due to the complexity of the human brain and the ethical considerations for in vivo studies. However, advances in nanomaterials and micro-/nano-microfabrication have opened up the chances for a deeper understanding of NoN ex vivo, one step closer to in vivo studies. This review therefore summarizes the latest advances in lab-on-chip microsystems for ex vivo NoN studies by focusing on the advanced materials, techniques, and models for ex vivo NoN studies. The essential methods for constructing lab-on-chip models are microfluidics and microelectrode arrays. Through combination with functional biomaterials and biocompatible materials, the microfluidics and microelectrode arrays enable the development of various models for ex vivo NoN studies. This review also includes the state-of-the-art brain slide and organoid-on-chip models. The end of this review discusses the previous issues and future perspectives for NoN studies.

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3D bioprinting for fabricating artificial skin tissue

Cited by 62 publications

References 112 publications

The 3D Bioprinted Scaffolds for Wound Healing

The 3D Bioprinted Scaffolds for Wound Healing

Simple and robust 3D bioprinting of full-thickness human skin tissue

Lab-on-Chip Microsystems for Ex Vivo Network of Neurons Studies: A Review

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