3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers

Du, Mingchun; Chen, Bing; Meng, Qing‐Yuan; Liu, Sumei; Zheng, Xiongfei; Zhang, Cheng; Wang, Heran; Li, Hongyi; Wang, Nuo; Dai, Jianwu

doi:10.1088/1758-5090/7/4/044104

Cited by 130 publications

(105 citation statements)

References 60 publications

(70 reference statements)

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“…Currently, there are numerous studies that focus on 3D bioprinting of cells and scaffold materials together to create 3D artificial constructs for organ transplantation and drug screening [15,16]. 3D bioprinting is not only useful for 3D cell interaction studies, but also it potentially helps in regeneration therapy.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications

et al. 2016

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Gelatin methacryloyl (GelMA) has been increasingly considered as an important bioink material due to its tailorable mechanical properties, good biocompatibility, and ability to be photopolymerized in situ as well as printability. GelMA can be classified into two types: type A GelMA (a product from acid treatment) and type B GelMA (a product from alkali treatment). In current literature, there is little research on the comparison of type A GelMA and type B GelMA in terms of synthesis, rheological properties, and printability for bioink applications. Here, we report the synthesis, rheological properties, and printability of types A and B GelMA. Types A and B GelMA samples with different degrees of substitution (DS) were prepared in a controllable manner by a time-lapse loading method of methacrylic anhydride (MAA) and different feed ratios of MAA to gelatin. Type B GelMA tended to have a slightly higher DS compared to type A GelMA, especially in a lower feed ratio of MAA to gelatin. All the type A and type B GelMA solutions with different DS exhibited shear thinning behaviours at 37 °C. However, only GelMA with a high DS had an easy-to-extrude feature at room temperature. The cell-laden printed constructs of types A and B GelMA at 20% w/v showed around 75% cell viability.

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Section: Introductionmentioning

confidence: 99%

Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications

et al. 2016

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“…The resulting self-standing structures could then be permanently solidified via UV-initiated crosslinking (Fig. 7a) [136, 137]. Multi-layered microfibrous structures of GelMA hydrogels could be fabricated using this bioprinting system (Fig.…”

Section: Spatial Control Of Hydrogelsmentioning

confidence: 99%

“…(i) Viability and spreading of fibroblasts encapsulated in the bioprinted construct at Day 5. Reproduced with permission from: (a–e) [136, 137] (f–i) [138]. …”

Section: Figurementioning

confidence: 99%

Spatially and temporally controlled hydrogels for tissue engineering

Leijten

Seo

Yue

et al. 2017

Materials Science and Engineering: R: Reports

159

129

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Summary Recent years have seen tremendous advances in the field of hydrogel-based biomaterials. One of the most prominent revolutions in this field has been the integration of elements or techniques that enable spatial and temporal control over hydrogels’ properties and functions. Here, we critically review the emerging progress of spatiotemporal control over biomaterial properties towards the development of functional engineered tissue constructs. Specifically, we will highlight the main advances in the spatial control of biomaterials, such as surface modification, microfabrication, photo-patterning, and three-dimensional (3D) bioprinting, as well as advances in the temporal control of biomaterials, such as controlled release of molecules, photocleaving of proteins, and controlled hydrogel degradation. We believe that the development and integration of these techniques will drive the engineering of next-generation engineered tissues.

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“…To achieve this, the bioink materials can be modified/ functionalized with ECM components. In a recent study, collagen films were first grinded using a crushingparticle desk crusher and passed through a 38μm mesh to get collagen microfibers of length 22 ± 13μm [111] . These collagen microfibers were linked with bone morphogenetic protein-2 (BMP2) that contained collagen-binding domain (CBD-BMP2).…”

Section: Emerging Strategies In Bioprintingmentioning

confidence: 99%

“…The CBD-BMP2 was printed onto bone marrow mesenchymal stem cells-laden methacrylamide gels. It was reported that these bone marrow mesenchymal stem cells differentiated into osteocyte cells due to the presence of ECM components such as collagen and BMP-2 in CBD-BMP2 [111] . Cells are subjected to a mild stress (thermal or mechanical) during bioprinting that may affect the cell viability in the printed constructs.…”

Section: Emerging Strategies In Bioprintingmentioning

confidence: 99%

3D bioprinting technology for regenerative medicine applications

Sundaramurthi¹,

Rauf²,

Hauser³

2016

IJB

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Alternative strategies that overcome existing organ transplantation methods are of increasing importance because of ongoing demands and lack of adequate organ donors. Recent improvements in tissue engineering techniques offer improved solutions to this problem and will influence engineering and medicinal applications. Tissue engineering employs the synergy of cells, growth factors and scaffolds besides others with the aim to mimic the native extracellular matrix for tissue regeneration. Three-dimensional (3D) bioprinting has been explored to create organs for transplantation, medical implants, prosthetics, in vitro models and 3D tissue models for drug testing. In addition, it is emerging as a powerful technology to provide patients with severe disease conditions with personalized treatments. Challenges in tissue engineering include the development of 3D scaffolds that closely resemble native tissues. In this review, existing printing methods such as extrusion-based, robotic dispensing, cellular inkjet, laser-assisted printing and integrated tissue organ printing (ITOP) are examined. Also, natural and synthetic polymers and their blends as well as peptides that are exploited as bioinks are discussed with emphasis on regenerative medicine applications. Furthermore, applications of 3D bioprinting in regenerative medicine, evolving strategies and future perspectives are summarized.

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3D bioprinting of BMSC-laden methacrylamide gelatin scaffolds with CBD-BMP2-collagen microfibers

Cited by 130 publications

References 60 publications

Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications

Synthesis and Characterization of Types A and B Gelatin Methacryloyl for Bioink Applications

Spatially and temporally controlled hydrogels for tissue engineering

3D bioprinting technology for regenerative medicine applications

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