3D bioprinting of collagen to rebuild components of the human heart

Lee, Andrew; Hudson, Andrew R.; Shiwarski, Daniel J.; Tashman, Joshua W.; Hinton, Thomas J.; Yerneni, Saigopalakrishna S.; Bliley, Jacqueline M.; Campbell, Phil G.; Feinberg, Adam W.

doi:10.1126/science.aav9051

Cited by 1,363 publications

(1,446 citation statements)

References 27 publications

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“…3D‐printing technologies have been extensively used in different research fields such as energy storage devices, catalysis, electronics, microfluidics, and biotechnology . These printing technologies have enabled the creation of unique material and device structures that cannot be achieved by conventional methods .…”

Section: Methodsmentioning

confidence: 99%

3D‐Printed Structure Boosts the Kinetics and Intrinsic Capacitance of Pseudocapacitive Graphene Aerogels

et al. 2020

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The performance of pseudocapacitive electrodes at fast charging rates are typically limited by the slow kinetics of Faradaic reactions and sluggish ion diffusion in the bulk structure. This is particularly problematic for thick electrodes and electrodes highly loaded with active materials. Here, a surface‐functionalized 3D‐printed graphene aerogel (SF‐3D GA) is presented that achieves not only a benchmark areal capacitance of 2195 mF cm−2 at a high current density of 100 mA cm−2 but also an ultrahigh intrinsic capacitance of 309.1 µF cm−2 even at a high mass loading of 12.8 mg cm−2. Importantly, the kinetic analysis reveals that the capacitance of SF‐3D GA electrode is primarily (93.3%) contributed from fast kinetic processes. This is because the 3D‐printed electrode has an open structure that ensures excellent coverage of functional groups on carbon surface and facilitates the ion accessibility of these surface functional groups even at high current densities and large mass loading/electrode thickness. An asymmetric device assembled with SF‐3D GA as anode and 3D‐printed GA decorated with MnO2 as cathode achieves a remarkable energy density of 0.65 mWh cm−2 at an ultrahigh power density of 164.5 mW cm−2, outperforming carbon‐based supercapacitors operated at the same power density.

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Section: Methodsmentioning

confidence: 99%

3D‐Printed Structure Boosts the Kinetics and Intrinsic Capacitance of Pseudocapacitive Graphene Aerogels

et al. 2020

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“…Furthermore, it is beneficial to have a bath material that allows a controlled transition from solid to liquid state, as offered, for example, by thermoreversible systems, to facilitate the extraction of printed constructs with filigree structures. Collagen I, for instance, can be used as an ink, as it is soluble under acidic conditions and by printing it into a buffered bath the pH‐dependent fibrillogenesis and gelation is triggered, resulting in a stable construct that can be harvested as shown in Figure A . Another example of a biomaterial class are materials that crosslink with the help of a secondary component, such as alginate with calcium ions or fibrinogen with thrombin.…”

Section: Recent Progress For Controlling Shapementioning

confidence: 99%

“…The support bath, based on gelatin, allowed the deposition of a low viscous collagen ink, utilizing an in situ crosslinking approach, and fabrication of complex structures with high shape fidelity that closely resemble the CAD file templates. Reproduced with permission . Copyright 2019, AAAS.…”

Section: Recent Progress For Controlling Shapementioning

confidence: 99%

“…It has been demonstrated that an increase of viscosity of the sheared gel correlates with an improved resolution in XY dimensions, but with a decrease in Z dimension. Support baths based on gelatin, which have also been termed freeform reversible embedding of suspended hydrogels (FRESH), included a particle size of ≈25 µm . This enables cell‐free print resolution of filaments with diameters of 25 µm.…”

Section: Recent Progress For Controlling Shapementioning

confidence: 99%

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From Shape to Function: The Next Step in Bioprinting

et al. 2020

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In 2013, the “biofabrication window” was introduced to reflect the processing challenge for the fields of biofabrication and bioprinting. At that time, the lack of printable materials that could serve as cell‐laden bioinks, as well as the limitations of printing and assembly methods, presented a major constraint. However, recent developments have now resulted in the availability of a plethora of bioinks, new printing approaches, and the technological advancement of established techniques. Nevertheless, it remains largely unknown which materials and technical parameters are essential for the fabrication of intrinsically hierarchical cell–material constructs that truly mimic biologically functional tissue. In order to achieve this, it is urged that the field now shift its focus from materials and technologies toward the biological development of the resulting constructs. Therefore, herein, the recent material and technological advances since the introduction of the biofabrication window are briefly summarized, i.e., approaches how to generate shape, to then focus the discussion on how to acquire the biological function within this context. In particular, a vision of how biological function can evolve from the possibility to determine shape is outlined.

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“…Due to the extremely low level of proliferation, a large number of cells are needed at the very beginning of the fabrication of cardiac muscle tissues. Lee et al successfully constructed a mini‐sized human ventricle model using human embryonic stem cell‐derived CMs, with the diameter of 5 mm and total height of 8 mm. With a final concentration of cells in the bioinks was 300 million cells mL −1 , the total number of cells for constructing such a model is ≈15 million.…”

Section: Challenges and Perspectivesmentioning

confidence: 99%

Nano‐ and Microfabrication for Engineering Native‐Like Muscle Tissues

et al. 2020

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Muscle tissues are characterized by highly organized 3D structures consisting of aligned myocytes and nonmyocytes. Several nano‐ and microfabrication technologies are used to engineer native‐like muscle tissues for muscle regeneration, the treatment of cardiovascular diseases, and in vitro disease modeling. In this paper, traditional tissue engineering methods and novel nano‐/microfabrication technologies for constructing muscle tissues are reviewed. Focus is given on the effects of nano‐/microfabricated architectures on the alignment of cells. In addition, issues of vascularization and cell–cell interaction in fabricating muscle tissues are discussed. Finally, common challenges of fabricating three types of muscle tissues and specific requirements for each type are reviewed, and perspectives are given for future studies.

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3D bioprinting of collagen to rebuild components of the human heart

Cited by 1,363 publications

References 27 publications

3D‐Printed Structure Boosts the Kinetics and Intrinsic Capacitance of Pseudocapacitive Graphene Aerogels

3D‐Printed Structure Boosts the Kinetics and Intrinsic Capacitance of Pseudocapacitive Graphene Aerogels

From Shape to Function: The Next Step in Bioprinting

Nano‐ and Microfabrication for Engineering Native‐Like Muscle Tissues

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