3D bioprinting of GelMA with enhanced extrusion printability through coupling sacrificial carrageenan

Wang, Xueping; Jiang, Jinhong; Yuan, Chenhui; Gu, Lin; Zhang, XinYu; Yao, Yudong; Shao, Lei

doi:10.1039/d3bm01489d

Cited by 4 publications

(4 citation statements)

References 35 publications

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“…The GelMA was synthesized as the same procedure reported [38]. Briefly, (1) 100 ml 10% (w/v) gelatin solution prepared with PBS at 50 • C.…”

Section: Methodsmentioning

confidence: 99%

Direct 3D printing of freeform anisotropic bioactive structure based on shear-oriented ink system

Yuan,

Jiang,

Zhang

et al. 2024

Biofabrication

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Various anisotropic tissue structures exist in organisms, including muscle tissue, skin tissue, and nerve tissue. Replicating anisotropic tissue structures in vitro has posed a significant challenge. 3D printing technology is often used to fabricate biomimetic structures due to its advantages in manufacturing principle. However, direct 3D printing of freeform anisotropic bioactive structures has not been reported. To tackle this challenge, we developed a ternary F/G/P ink system that integrates the printability of Pluronic F127 (F), the robust bioactivity and photocrosslinking properties of GelMA (G), and the shear-induced alignment functionality of high-molecular-weight PEG (P). And through this strategic ternary system combination, freeform anisotropic tissue structures can be 3D printed directly. Moreover, these anisotropic structures exhibit excellent bioactivity, and promote orientational growth of different cells. This advancement holds promise for the repair and replacement of anisotropic tissues within the human body.

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“…The GelMA was synthesized as the same procedure reported [38]. Briefly, (1) 100 ml 10% (w/v) gelatin solution prepared with PBS at 50 • C.…”

Section: Methodsmentioning

confidence: 99%

Direct 3D printing of freeform anisotropic bioactive structure based on shear-oriented ink system

Yuan,

Jiang,

Zhang

et al. 2024

Biofabrication

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“…The main challenge in bioprinting of low viscosity bioinks is producing constructs with high printing accuracy. In the case of Gel-Ma, it is possible to take advantage of its thermoresponsiveness to improve filament formation [21] or use various sacrificial components [19,20]. However, the low viscosity of PHEG-Tyr-based bioinks cannot be influenced by temperature, leading to its poor printability in the extrusion bioprinting experiment using a Temperature-controlled printhead at 25 • C. (figure 10-1) Nevertheless, in this work, we wanted to show an alternative method that can be used for low viscosity bioinks without any additional steps, known as droplet-based bioprinting.…”

Section: Bioprinting Of the Proposed Bioinksmentioning

confidence: 99%

“…Natural polymers, such as gelatin methacrylate (Gel-Ma), are commonly used as bioinks in 3D bioprinting due to their photocrosslinkable methacryloyl groups and intrinsic cell adhesive sites [9,10]. However, there are several limitations to the use of Gel-Ma for 3D bioprinting, like its low viscosity at room and physiological temperatures [19,20] leading to poor filament formation. That can be improved by Gel-Ma physical gelation at lower temperatures.…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, even slight temperature variations can affect the viability of exposed laden cells [23]. Other options to overcome the challenge of printing with low viscosity bioinks are sacrificial additives [19,20] or embedded bioprinting with support baths [8]. Alternately, methods like droplet-based bioprinting can be employed to avoid extra steps in the process, offering simple, versatile printing technology with up to 90% post-printing cell viability [24].…”

Section: Introductionmentioning

confidence: 99%

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Fully synthetic, tunable poly(α-amino acids) as the base of bioinks curable by visible light

Golunova,

Dvořáková,

Velychkivska

et al. 2024

Biomed. Mater.

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Bioinks play a crucial role in tissue engineering, influencing mechanical and chemical properties of the printed scaffold as well as the behavior of encapsulated cells. Recently, there has been a shift from animal origin materials to their synthetic alternatives. In this context, we present here bioinks based on fully synthetic and biodegradable poly(α,L-amino acids) (PolyAA) as an alternative to animal-based gelatin methacrylate (Gel-Ma) bioinks. Additionally, we first reported the possibility of the visible light photoinitiated incorporation of the bifunctional cell adhesive RGD peptide into the PolyAA hydrogel matrix. The obtained hydrogels are shown to be cytocompatible, and their mechanical properties closely resemble those of gelatin methacrylate-based scaffolds. Moreover, combining the unique properties of PolyAA-based bioinks, the photocrosslinking strategy, and the use of droplet-based printing allows the printing of constructs with high shape fidelity and structural integrity from low-viscosity bioinks without using any sacrificial components. Overall, presented PolyAA-based materials are a promising and versatile toolbox that extends the range of bioinks for droplet bioprinting.

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Dual‐Crosslinked Bioactive Hydrogel Scaffold for Accelerated Repair of Genital Tract Defect

Wang,

Cheng,

Chen

et al. 2024

Adv Funct Materials

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Reproductive health concerns like Mayer‐Rokitansky‐Küster‐Hauser (MRKH) syndrome are prevalent in today's society. MRKH syndrome is a condition that severely affects women's sexual life, fertility, and mental health and has a high prevalence of one out of 5000 female births. Vaginoplasty is the primary method to regain patients’ reproductive health. However, conventional vaginoplasty faces various challenges, including complex and non‐customized treatment procedures causing intense pains and complications. To bring new advances to vaginoplasty, a 3D‐printed hydrogel scaffold is developed to provide satisfactory mechanical support and bioactivity for accelerating defect repair after surgery. The hydrogel scaffold consisting of gelatin methacryloyl (gelMA) and carrageenan (Car) is custom 3D‐printed using an ambient temperature printing system. Furthermore, the scaffold undergoes dual‐crosslinking through chemical crosslinking of gelMA and ionic crosslinking of Car with magnesium ions (Mg2+). This dual‐crosslinking strategy substantially improves the overall mechanical properties of the scaffold and introduces bioactive Mg2+. The sustained release of Mg2+ plus the extracts from the dual‐crosslinked scaffold significantly promotes cell proliferation, migration and angiogenesis. In a preclinical rat model with penetrating genital tract defects mimicking vaginoplasty, the implantation of dual‐crosslinked scaffold repairs the penetrating wounds to near‐normal levels within one week, showing potential as an alternative for better regaining reproductive health.

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3D bioprinting of GelMA with enhanced extrusion printability through coupling sacrificial carrageenan

Abstract: This study developed a novel 3D bioprinting platform using GelMA and carrageenan, which enhanced printability at 28 °C, without changing cell viability, and showed potential for promoting osteogenic differentiation of mesenchymal stem cells.

Cited by 4 publications

References 35 publications

Direct 3D printing of freeform anisotropic bioactive structure based on shear-oriented ink system

Direct 3D printing of freeform anisotropic bioactive structure based on shear-oriented ink system

Fully synthetic, tunable poly(α-amino acids) as the base of bioinks curable by visible light

Dual‐Crosslinked Bioactive Hydrogel Scaffold for Accelerated Repair of Genital Tract Defect

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