3D bioprinting of tissue constructs employing dual crosslinking of decellularized extracellular matrix hydrogel

Yeleswarapu, Sriya; Dash, Abhishek; Chameettachal, Shibu; Pati, Falguni

doi:10.1016/j.bioadv.2023.213494

Cited by 19 publications

(3 citation statements)

References 36 publications

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“…Furthermore, the integration of many cell types into a single scaffold is made possible by 3D printing. Because it enables the incorporation of diverse cell populations like keratinocytes, fibroblasts, and endothelial cells, this capability is especially helpful in skin tissue engineering . The intricate cellular interactions required for skin regeneration can be replicated by cocultivating these cells in a scaffold that is 3D printed. , A few limitations associated with electrospinning include poor cell infiltration and migration as a result of the close packing of scaffold fibers, the toxicity of the residual solvent, and the low mechanical strength of the scaffolds for load-bearing applications. − …”

Section: Introductionmentioning

confidence: 99%

RETRACTED: 3D Printing of Alginate/Chitosan-Based Scaffold Empowered by Tyrosol-Loaded Niosome for Wound Healing Applications: In Vitro and In Vivo Performances

Beram,

Ali,

Mesbahian

et al. 2024

ACS Appl. Bio Mater.

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spinning and 3D printing techniques for wound healing applications. The niosomes, measuring 185.40 ± 6.40 nm with a polydispersity index of 0.168 ± 0.012, encapsulated tyrosol with an efficiency of 77.54 ± 1.25%. The scaffold's microsized porous structure (600−900 μm) enhances water absorption, promoting cell adhesion, migration, and proliferation. Mechanical property assessments revealed the scaffold's enhanced resilience, with niosomes increasing the compressive strength, modulus, and strain to failure, indicative of its suitability for wound healing. Controlled tyrosol release was demonstrated in vitro, essential for therapeutic efficacy. The scaffold exhibited significant antibacterial activity against Pseudomonas aeruginosa and Staphylococcus aureus, with substantial biofilm inhibition and downregulation of bacterial genes (ndvb and icab). A wound healing assay highlighted a notable increase in MMP-2 and MMP-9 mRNA expression and the wound closure area (69.35 ± 2.21%

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Section: Introductionmentioning

confidence: 99%

RETRACTED: 3D Printing of Alginate/Chitosan-Based Scaffold Empowered by Tyrosol-Loaded Niosome for Wound Healing Applications: In Vitro and In Vivo Performances

Beram,

Ali,

Mesbahian

et al. 2024

ACS Appl. Bio Mater.

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“…Due to the printability of dECM, researchers constructed different types of bioengineered 3D structures based on dECM, showing translational potential for promoting tissue repair ( Hwang et al, 2022 ). However, the low viscosity and long gelation time may result in the poor shape fidelity of dECM hydrogel-based 3D structure ( Yeleswarapu et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Recent advances in fabrication of dECM-based composite materials for skin tissue engineering

Xu,

Cao,

Duan

et al. 2024

Front. Bioeng. Biotechnol.

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Chronic wound management is an intractable medical and social problem, affecting the health of millions worldwide. Decellularized extracellular matrix (dECM)-based materials possess remarkable biological properties for tissue regeneration, which have been used as commercial products for skin regeneration in clinics. However, the complex external environment and the longer chronic wound-healing process hinder the application of pure dECM materials. dECM-based composite materials are constructed to promote the healing process of different wounds, showing noteworthy functions, such as anti-microbial activity and suitable degradability. Moreover, fabrication technologies for designing wound dressings with various forms have expanded the application of dECM-based composite materials. This review provides a summary of the recent fabrication technologies for building dECM-based composite materials, highlighting advances in dECM-based molded hydrogels, electrospun fibers, and bio-printed scaffolds in managing wounds. The associated challenges and prospects in the clinical application of dECM-based composite materials for wound healing are finally discussed.

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“…Furthermore, the dECM solution could be transformed into hydrogel at an appropriate temperature with three-dimensional (3D) network structures, providing a unique and effective microenvironment for the developing cells and surrounding tissue [ 26 , 27 ]. However, the unsuitable mechanical rigidity and rapid biodegradability still hinder the application of pure dECM [ 28 , 29 ]. Bioengineered composite scaffolds composed of dECM with different kinds of biomaterials have provided excellent prospects in regenerative medicine and resulted in hopeful achievements of bioengineered constructions for tissue repair, reconstruction and regeneration.…”

Section: Introductionmentioning

confidence: 99%

Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine

Xu,

Kankala,

Wang

et al. 2023

Regenerative Biomaterials

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Despite the considerable advancements in fabricating polymeric-based scaffolds for tissue engineering, the clinical transformation of these scaffolds remained a big challenge because of the difficulty of simulating native organs/tissues' microenvironment. As a kind of natural tissue-derived biomaterials, decellularized extracellular matrix (dECM)-based scaffolds have gained attention due to their unique biomimetic properties, providing a specific microenvironment suitable for promoting cell proliferation, migration, attachment, and regulating differentiation. The medical applications of dECM-based scaffolds have addressed critical challenges, including poor mechanical strength and insufficient stability. For promoting the reconstruction of damaged tissues or organs, different types of dECM-based composite platforms have been designed to mimic tissue microenvironment, including by integrating with natural polymer or/and syntenic polymer or adding bioactive factors. In this review, we summarized the research progress of dECM-based composite scaffolds in regenerative medicine, highlighting the critical challenges and future perspectives related to the medical application of these composite materials.

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3D bioprinting of tissue constructs employing dual crosslinking of decellularized extracellular matrix hydrogel

Cited by 19 publications

References 36 publications

RETRACTED: 3D Printing of Alginate/Chitosan-Based Scaffold Empowered by Tyrosol-Loaded Niosome for Wound Healing Applications: In Vitro and In Vivo Performances

RETRACTED: 3D Printing of Alginate/Chitosan-Based Scaffold Empowered by Tyrosol-Loaded Niosome for Wound Healing Applications: In Vitro and In Vivo Performances

Recent advances in fabrication of dECM-based composite materials for skin tissue engineering

Decellularized extracellular matrix-based composite scaffolds for tissue engineering and regenerative medicine

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