3D Bioprinting of Vascularized, Heterogeneous Cell‐Laden Tissue Constructs

Kolesky, David B.; Truby, Ryan L.; Gladman, A. Sydney; Busbee, Travis A.; Homan, Kimberly A.; Lewis, Jennifer A.

doi:10.1002/adma.201305506

Cited by 1,844 publications

(1,561 citation statements)

References 35 publications

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“…Accordingly, single-component Pluronic gels are not used to print persistent cellularized structures, instead they have found application as cell-free "fugitive inks" for vascularized scaffold formation. [ 15 ] Conversely, chemically crosslinked gel systems offer excellent structural fi delity in aqueous solutions, but can be limited by poor compatibility with fi ber extrusion and layer-by-layer printing. For example, the linear polysaccharide alginate, found naturally in the cell walls of brown algae, can be rapidly crosslinked through chelation of divalent cations by the carboxylic acid groups founds on adjacent strands of the component β-D -mannuronate or α-L -guluronate epimers.…”

Section: Doi: 101002/adhm201600022mentioning

confidence: 99%

3D Bioprinting Using a Templated Porous Bioink

Armstrong

Burke

Carter

et al. 2016

Adv Healthcare Materials

163

111

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3D cell printing (bioprinting) is rapidly emerging as a key biofabrication strategy for engineering tissue constructs with physiological form and complexity. [1][2][3][4] In practice, this process involves layer-by-layer deposition of a cell-laden bioink resulting in the additive manufacture of a patterned architecture with different cell types, growth factors, or mechanical cues, which are positioned with far greater precision than can be achieved with conventional scaffold-based tissue engineering. [ 5 ] While there have been signifi cant advances in printing technology, [ 6,7 ] progress has been limited by the rate of development of bioinks that are compatible with both 3D printing and tissue engineering. [ 8 ] These materials must be able to withstand extrusion, maintain structural fi delity for long time periods, and permit adequate nutrient diffusion, all under cytocompatible conditions. Due to their intrinsic porosity and capacity for high nutrient loading, hydrogels are the most promising candidate for bioink design, [ 9 ] particularly when gelation can be externally triggered using chemical bonding, [ 10 ] photoinduced crosslinking, [ 11 ] thermal setting, [ 12 ] or shear-thinning. [ 13 ] However, integrating these factors into a system while maintaining printability, structural persistence, and cell viability, is an enduring challenge. [ 14 ] Pluronic block copolymers of poly(ethylene oxide-b-propylene oxide-b-ethylene oxide) present a possible pathway to print gelation, as they undergo a sol-gel transition upon heating near physiological temperatures. Here, elevating the temperature of these non-ionic surfactants reduces the

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Section: Doi: 101002/adhm201600022mentioning

confidence: 99%

3D Bioprinting Using a Templated Porous Bioink

Armstrong

Burke

Carter

et al. 2016

Adv Healthcare Materials

163

111

View full text Add to dashboard Cite

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“…For applications where a higher resolution is required, many techniques and products are available to adjust the rheological behavior of the PDMS precursor. [5,24,[43][44][45][46][47] The development of the hydrogel precursor for our extrusion printing was more demanding and required a balance between conductivity, stability, and rheological characteristics.…”

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confidence: 99%

3D Printing of Transparent and Conductive Heterogeneous Hydrogel–Elastomer Systems

et al. 2017

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A hydrogel–dielectric‐elastomer system, polyacrylamide and poly(dimethylsiloxane) (PDMS), is adapted for extrusion printing for integrated device fabrication. A lithium‐chloride‐containing hydrogel printing ink is developed and printed onto treated PDMS with no visible signs of delamination and geometrically scaling resistance under moderate uniaxial tension and fatigue. A variety of designs are demonstrated, including a resistive strain gauge and an ionic cable.

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“…Contrary to bioassembly, where the minimum fabrication units are pre-formed cell building blocks, bioprinting makes use of fabrication units down to molecular level [78]. Bioprinting processes are capable of printing living and non-living materials in a computercontrolled and automated manner with high levels of resolution, accuracy and reproducibility, enabling the generation of biological substitutes with intricate architectures, precise geometrical configurations and biomechanical heterogeneity [16,80,91,92]. Bioprinting technologies can be classified in three main categories: inkjet bioprinting, laser-assisted bioprinting and extrusion bioprinting (Table 1) [111,125,191].…”

Section: Bioprinting Technologiesmentioning

confidence: 99%

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

et al. 2017

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Bioprinting technologies are powerful additive biofabrication techniques to produce cellular constructs for skin tissue engineering owing to their unique ability to precisely pattern living and non-living materials in predefined spatial locations. This unique feature, combined with the computer controlled printing and medical imaging techniques, enable researchers and clinicians to generate patient specific constructs partly replicating the intricate compositional and architectural organization of the skin. Bioprinting has been used to automatically dispense hydrogels with skin cells located in prescribed sites that promote skin formation in vitro and in vivo. Current skin bioprinting approaches mostly rely on the sequential printing of fibroblasts and keratinocytes embedded within a homogeneous hydrogel. Although such approaches have already been translated to pre-clinical scenarios, they still present limitations in terms of fully replicating the cellular and extracellular matrix (ECM) heterogeneity in native skin. The success of bioprinting for skin repair strongly depends on the design of printable bioinks capable of supporting the function of printed cells and stimulating the production of new ECM components. To better mimic the human skin, novel developments in dedicated bioprinting technologies, in the design of bioinks, as well as in the printing of vascularised constructs are necessary. This paper presents an overview regarding the use of bioprinting for skin tissue engineering applications. The operating principles of bioprinting technologies are outlined along with requirements of printed skin constructs. Finally, preclinical results are summarized and future perspectives for the field are highlighted.

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3D Bioprinting of Vascularized, Heterogeneous Cell‐Laden Tissue Constructs

Cited by 1,844 publications

References 35 publications

3D Bioprinting Using a Templated Porous Bioink

3D Bioprinting Using a Templated Porous Bioink

3D Printing of Transparent and Conductive Heterogeneous Hydrogel–Elastomer Systems

Advances in bioprinted cell-laden hydrogels for skin tissue engineering

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