3D Carbon Nanotubes Spaced Graphene Aerogel Incorporated with Prussian Blue Nanoparticles for Real-Time Detection of H₂O₂ Released from Living Cells

Abstract

Real-time detection of hydrogen peroxide (H2O2) released from living cells is of great value in further understanding the occurrence process of some diseases and searching for new therapeutic strategies. However, selective monitoring of H2O2 in living cells is still a huge challenge due to its low concentration, large diffusivity and high reactivity. Herein, three-dimensional carbon nanotubes spaced graphene aerogel incorporated with Prussian blue nanoparticles (3D PB NPs/G-CNTs) has been successfully synthesized via one-step mild temperature treatment, in which the PB NPs possess intrinsic peroxidase-like activity while the 3D porous structure of G-CNTs with large surface area and high conductivity can efficiently enhance catalytic performance. The composite exhibited good catalytic performances toward H2O2 with a sensitivity of 134.3 μA mM−1 cm−2 and a low detection limit of 95 nM in a linear range of 1–3161 μM. Furthermore, the proposed sensor was successfully used for real-time detection of H2O2 released from living cells, indicating the potential application of as-prepared 3D PB NPs/G-CNTs in monitoring the critical pathological process in living cells.

Single-Atom Iron Anchored on 2-D Graphene Carbon to Realize Bridge-Adsorption of O–O as Biomimetic Enzyme for Remarkably Sensitive Electrochemical Detection of H2O2

“…It is reported H 2 O 2 can be fast released from living cells upon the stimulation of PMA. The monitored current response by PMA stimulation was 1.05 μA, which corresponded to 13.32 μM H 2 O 2 according to the calibration curve in Figure b, and equaled 0.053 fmol released H 2 O 2 per cell, which is in good agreement with the previous report. , This also further confirms the high sensitivity of this sensor even as low as 0.05 fmol molecules.…”

“…In more detail, after injection of PMA (600 ng/mL) in 4 mL of PBS containing 1 × 10 6 of A549 living cells to stimulate H 2 O 2 release, instant current response was observed as shown in curve a (Figure 5d), while control experiments showed that no 5b, and equaled 0.053 fmol released H 2 O 2 per cell, which is in good agreement with the previous report. 24,25 This also further confirms the high sensitivity of this sensor even as low as 0.05 fmol molecules. Iron single atomic site catalyst dispersed on 1-D carbon nanowires (Fe-SASC/NW) as a nanozyme has been reported to sensing H 2 O 2 but only showing a sensitivity of 6.5 μA mM −1 cm 2 .…”

A Highly Stable Graphene‐Based Metal Oxide Nano Enzyme with the Peroxidase‐Mimetic Activity for Equipment‐Free Diagnosis of Diabetic Patients

“…[17] An intriguing application of the nanoparticle-loaded GO composites is their use as enzyme mimetics, perticularly for peroxidase mimicking. Various studies have investigated the peroxidase mimicking activity of Cu ions, [18,19] Ag/rGO nanocomposites, [20] 3D PB NPs/G-CNTs, [21] 3D PB/GMA (Prussian blue-doped RGO/MXene composite aerogel) [22] nitrogen-doped GO quantum dots, [23] GOÀ Fe 2 O 3 hybrids, [15] MoS 2 /GO hybrid, [24] polystyrene sulfonate/Pt/graphene nanosheets [17] for colorimetric detection of H 2 O 2 .…”

Rational Confinement of Yttrium Vanadate within Three-Dimensional Graphene Aerogel: Electrochemical Analysis of Monoamine Neurotransmitter (Dopamine)

“…In addition, GA shows excellent structural stability, which can tolerate the damage during the electrochemical reaction. Apart from the properties, the GA offers excellent potential in applying electrochemical sensors. − However, there is no previous literature of YVO/GA nanocomposite being applied in the electrochemical sensor. In this spirit, YVO/GA nanocomposite was prepared through a sonochemical method and used as an electrode material toward the detection of DA.…”