3D cell bioprinting of self-assembling peptide-based hydrogels

Raphael, Bella; Khalil, Tony; Workman, Victoria L.; Smith, Andrew M.; Brown, Cameron; Streuli, Charles; Saiani, Alberto; Domingos, Marco

doi:10.1016/j.matlet.2016.12.127

Cited by 107 publications

(79 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Supramolecular hydrogels are particularly attractive for extrusion-based printing as they could flow under shear and self-heal immediately after printing, leading to high print fidelity. In addition to guest–host bonding, self-assembling peptides (Raphael et al, 2017) and polypeptide–DNA hydrogels (Li et al, 2015) are other emerging candidates for bioink design.…”

Section: Currently Available Bioinksmentioning

confidence: 99%

Recent Advances in Bioink Design for 3D Bioprinting of Tissues and Organs

Guvendiren

2017

Front. Bioeng. Biotechnol.

379

258

View full text Add to dashboard Cite

There is a growing demand for alternative fabrication approaches to develop tissues and organs as conventional techniques are not capable of fabricating constructs with required structural, mechanical, and biological complexity. 3D bioprinting offers great potential to fabricate highly complex constructs with precise control of structure, mechanics, and biological matter [i.e., cells and extracellular matrix (ECM) components]. 3D bioprinting is an additive manufacturing approach that utilizes a “bioink” to fabricate devices and scaffolds in a layer-by-layer manner. 3D bioprinting allows printing of a cell suspension into a tissue construct with or without a scaffold support. The most common bioinks are cell-laden hydrogels, decellulerized ECM-based solutions, and cell suspensions. In this mini review, a brief description and comparison of the bioprinting methods, including extrusion-based, droplet-based, and laser-based bioprinting, with particular focus on bioink design requirements are presented. We also present the current state of the art in bioink design including the challenges and future directions.

show abstract

Section: Currently Available Bioinksmentioning

confidence: 99%

Recent Advances in Bioink Design for 3D Bioprinting of Tissues and Organs

Guvendiren

2017

Front. Bioeng. Biotechnol.

379

258

View full text Add to dashboard Cite

show abstract

“…As such, they could be used as sacrificial or fugitive inks 33 . 3D printed architectures made of hydrogels that are not based on polymers but based on small gelling molecules are still scarcely described in literature 23,[34][35][36][37][38][39] and most of them are based on self-assembling gelling peptides. In these examples, extrusion printing relies on the thixotropy and/or in-, on post-process gelation (typically, the change of pH after extrusion leads to the gelation of the gelling peptides) or on electrostatic self-assembly similar to the layer-by-layer technique 38 .…”

Section: Introductionmentioning

confidence: 99%

3D printing of a biocompatible low molecular weight supramolecular hydrogel by dimethylsulfoxide water solvent exchange

Chalard

Mauduit

Souleille

et al. 2020

Additive Manufacturing

View full text Add to dashboard Cite

A fragile and non-thixotropic biocompatible low molecular weight gel is printed in 3D structures by a solvent exchange process. The 3D printing process is based on the continuous extrusion of a solution of a small amphiphile molecule, N-heptyl-D-galactonamide, in dimethylsulfoxide, that forms a gel in contact with water. The diffusion of water in the dimethylsulfoxide / N-heptyl-D-galactonamide solution triggers the self-assembly of the molecule into supramolecular fibers and the setting of the ink. The conditions for getting a well-defined pattern and the dimensions of the constructs have been determined. The resulting constructs can be easily dissolved, orienting its application as a sacrificial ink or a temporary support. This method opens the way to the injection and the 3D printing of other fragile and non-thixotropic supramolecular hydrogels.

show abstract

“…After deposition, the materials can recover and thus retain the shape after extrusion. Examples include polyisocyanate hydrogels used as biomaterial inks or oligo‐peptide bioinks . Besides polypeptides also proteins are used as physical gels for bioink development.…”

Section: Recent Progress For Controlling Shapementioning

confidence: 98%

From Shape to Function: The Next Step in Bioprinting

et al. 2020

View full text Add to dashboard Cite

In 2013, the “biofabrication window” was introduced to reflect the processing challenge for the fields of biofabrication and bioprinting. At that time, the lack of printable materials that could serve as cell‐laden bioinks, as well as the limitations of printing and assembly methods, presented a major constraint. However, recent developments have now resulted in the availability of a plethora of bioinks, new printing approaches, and the technological advancement of established techniques. Nevertheless, it remains largely unknown which materials and technical parameters are essential for the fabrication of intrinsically hierarchical cell–material constructs that truly mimic biologically functional tissue. In order to achieve this, it is urged that the field now shift its focus from materials and technologies toward the biological development of the resulting constructs. Therefore, herein, the recent material and technological advances since the introduction of the biofabrication window are briefly summarized, i.e., approaches how to generate shape, to then focus the discussion on how to acquire the biological function within this context. In particular, a vision of how biological function can evolve from the possibility to determine shape is outlined.

show abstract

3D cell bioprinting of self-assembling peptide-based hydrogels

Cited by 107 publications

References 35 publications

Recent Advances in Bioink Design for 3D Bioprinting of Tissues and Organs

Recent Advances in Bioink Design for 3D Bioprinting of Tissues and Organs

3D printing of a biocompatible low molecular weight supramolecular hydrogel by dimethylsulfoxide water solvent exchange

From Shape to Function: The Next Step in Bioprinting

Contact Info

Product

Resources

About