3D culturing of human adipose-derived stem cells enhances their pluripotency and differentiation abilities

Neuronal Cell Differentiation of Human Dental Pulp Stem Cells on Synthetic Polymeric Surfaces Coated With ECM Proteins

“…The cells were incubated with anti-CD markers (1:500 dilution) or isotype antibodies (1:500 dilution) for 45 min, and the solution was centrifuged at 450 × g for 8 min. The cells were transferred into a phosphate-buffered saline solution and evaluated utilizing flow cytometry as previously described ( Sung et al, 2021a ).…”

“…In our previous study ( Sung et al, 2021a ), human adipose-derived stem cells (hADSCs) were cultivated sequentially in a two-dimensional (2D) biomaterial surface and in a three-dimensional (3D) culturing condition. hADSCs cultured in 3D culture conditions showed higher expression of differentiation markers of osteogenic cells and chondrogenic cells than that of hADSCs cultured in 2D culture conditions when hADSCs were differentiated into osteogenic cells and chondrogenic cells ( Sung et al, 2021a ).…”

“…In our previous study ( Sung et al, 2021a ), human adipose-derived stem cells (hADSCs) were cultivated sequentially in a two-dimensional (2D) biomaterial surface and in a three-dimensional (3D) culturing condition. hADSCs cultured in 3D culture conditions showed higher expression of differentiation markers of osteogenic cells and chondrogenic cells than that of hADSCs cultured in 2D culture conditions when hADSCs were differentiated into osteogenic cells and chondrogenic cells ( Sung et al, 2021a ). However, the pluripotency and differentiation ability of hADSCs were extremely decreased when they were transferred from 3D culture conditions to 2D culture conditions and vice versa, which suggested that hADSCs have reversible characteristics in terms of their pluripotent characteristics and differentiation potential that depend on their environmental niche in 3D and 2D cultures.…”

Effect of extracellular matrix proteins on the differentiation of human pluripotent stem cells into mesenchymal stem cells

“…hMSCs express specific surface markers, such as CD44, CD73, CD90 and CD105, but do not express hematopoietic stem cell surface markers, such as CD34. 36–42 Therefore, the expression of MSC surface markers (CD44, CD73, CD90 and CD105) and a hematopoietic stem cell surface marker (CD34) on hPSC-derived hMSCs cultured on ECM-coated dishes and TCP dishes was evaluated using flow cytometry to characterize the hPSC-derived hMSCs (Fig. 4 and Fig.…”

Adipose tissue in bone regeneration - stem cell source and beyond

“…Ectodermal (neurons, glia and Schwan cells) and endodermal (hepatocytes and pancreatic beta islet cells) ADSC conversion has been obtained[ 91 ]. A subset of ADSCs was shown to express markers of pluripotency (Sox2, Nanog, and OCT4) and to differentiate into mesodermal and extramesodermal lineages, especially when cultured in three-dimensional suspension culture[ 92 ]. An important feature of putative ADSCs is their growth factor and immunomodulatory cytokine release.…”