3D engineered tissue models for studying human-specific infectious viral diseases

Hwang, Kyeong Seob; Seo, Eun U; Choi, Nakwon; Kim, Jongbaeg; Kim, Hong Nam

doi:10.1016/j.bioactmat.2022.09.010

Cited by 3 publications

(2 citation statements)

References 243 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[35][36][37] Disease modeling studies differ from engineering healthy tissue models by using different cell types and/or disease-inducing agents. [38,39] Controlled delivery studies focus mainly on embedding cargo carrying nano/microparticles into biomaterials. [40,41] Interestingly, this approach has recently also been introduced into traditional tissue engineering applications for controlled delivery of biological cues and for improving control over stem cell fate, which has resulted in a new field named particle-assisted tissue engineering.…”

Section: Improved Biofabrication Techniquesmentioning

confidence: 99%

Toward Artificial Cell‐Mediated Tissue Engineering: A New Perspective

Sümbelli,

Mason,

van Hest

2023

Advanced Biology

View full text Add to dashboard Cite

The fast‐growing pace of regenerative medicine research has allowed the development of a range of novel approaches to tissue engineering applications. Until recently, the main points of interest in the majority of studies have been to combine different materials to control cellular behavior and use different techniques to optimize tissue formation, from 3‐D bioprinting to in situ regeneration. However, with the increase of the understanding of the fundamentals of cellular organization, tissue development, and regeneration, has also come the realization that for the next step in tissue engineering, a higher level of spatiotemporal control on cell‐matrix interactions is required. It is proposed that the combination of artificial cell research with tissue engineering could provide a route toward control over complex tissue development. By equipping artificial cells with the underlying mechanisms of cellular functions, such as communication mechanisms, migration behavior, or the coherent behavior of cells depending on the surrounding matrix properties, they can be applied in instructing native cells into desired differentiation behavior at a resolution not to be attained with traditional matrix materials.

show abstract

Section: Improved Biofabrication Techniquesmentioning

confidence: 99%

Toward Artificial Cell‐Mediated Tissue Engineering: A New Perspective

Sümbelli,

Mason,

van Hest

2023

Advanced Biology

View full text Add to dashboard Cite

show abstract

“…Although mechanisms of infection have been studied in animal models as well as in 2D cell cultures, there are few organ-specific models of pathogenesis, especially for humans, which would require a mimic of a human organ. There are now engineered 3D models of human tissues that mimic many aspects of the physiology of human organs, including the human origin of the cells, the 3D structure, the response to physiological stimuli, and the tight cell-cell interactions (63,64). Further, de Dios-Figueroa GT et al reported that 3D cell culture models mimic major in vivo tissue properties such as virus-host interactions.…”

Section: Infectious Diseasementioning

confidence: 99%

Deciphering the underlying mechanism of liver diseases through utilization of multicellular hepatic spheroid models

Kim

Lee

Seo

2023

BMB Rep

View full text Add to dashboard Cite

Hepatocellular carcinoma (HCC) is a very common form of cancer worldwide and is often fatal.Although the histopathology of HCC is characterized by metabolic pathophysiology, fibrosis, and cirrhosis, the focus of treatment has been on eliminating HCC. Recently, three-dimensional (3D) multicellular hepatic spheroid (MCHS) models have provided a) new therapeutic strategies for progressive fibrotic liver diseases, such as antifibrotic and anti-inflammatory drugs, b) molecular targets, and c) treatments for metabolic dysregulation. MCHS models provide a potent anti-cancer tool because they can mimic a) tumor complexity and heterogeneity, b) the 3D context of tumor cells, and c) the gradients of physiological parameters that are characteristic of tumors in vivo.However, the information provided by an MCTS model must always be considered in the context of tumors in vivo. This mini-review summarizes what is known about tumor HCC heterogeneity and complexity and the advances provided by MCHS models for innovations in drug development to combat liver diseases. clinical utility of drugs that proved effective in vitro is low. Also, assays of liver function, including cell polarization, albumin synthesis, bile secretion, and urea synthesis, are better when they are performed in three-dimensional (3D) cell cultures that more accurately reflect the normal context of liver cells (7). Similarly, 3D cells provide a better model system for cancer drug discovery.The interactions between the tumor and the tumor microenvironment (TME) play a critical role in cell differentiation, tumorigenesis, metastasis, and the efficacy of drug therapies. Therefore it was important to develop a 3D TME platform to discover new therapeutics for liver cancer drug discovery. These platforms are complex and expensive to produce; however, the recently developed multicellular tumor spheroid (MCTS) models provide a new platform for highthroughput screening (HTS) of drugs to treat a variety of diseases, including cancers and infections.The development of 3D multicellular hepatic spheroid (MCHS) models has had a major impact on drug discovery and the identification of novel targets for the treatment of HCC and fibrosis. MCHS models should greatly increase the number of potential new drugs to treat HCC and reduce the time to drug discovery.In this review, we describe the influence of components of the TME on tumorigenesis, fibrogenesis, and chemoresistance in HCC, as well as advanced strategies that exploit 3D multicellular spheroid models to identify novel cancer targets and therapeutics.

show abstract

Nonhuman primate models of pediatric viral diseases

Vijayan K. K.,

De Paris

2024

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Infectious diseases are the leading cause of death in infants and children under 5 years of age. In utero exposure to viruses can lead to spontaneous abortion, preterm birth, congenital abnormalities or other developmental defects, often resulting in lifelong health sequalae. The underlying biological mechanisms are difficult to study in humans due to ethical concerns and limited sample access. Nonhuman primates (NHP) are closely related to humans, and pregnancy and immune ontogeny in infants are very similar to humans. Therefore, NHP are a highly relevant model for understanding fetal and postnatal virus-host interactions and to define immune mechanisms associated with increased morbidity and mortality in infants. We will discuss NHP models of viruses causing congenital infections, respiratory diseases in early life, and HIV. Cytomegalovirus (CMV) remains the most common cause of congenital defects worldwide. Measles is a vaccine-preventable disease, yet measles cases are resurging. Zika is an example of an emerging arbovirus with devastating consequences for the developing fetus and the surviving infant. Among the respiratory viruses, we will discuss influenza and Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). We will finish with HIV as an example of a lifelong infection without a cure or vaccine. The review will highlight (i) the impact of viral infections on fetal and infant immune development, (ii) how differences in infant and adult immune responses to infection alter disease outcome, and emphasize the invaluable contribution of pediatric NHP infection models to the design of effective treatment and prevention strategies, including vaccines, for human infants.

show abstract

3D engineered tissue models for studying human-specific infectious viral diseases

Cited by 3 publications

References 243 publications

Toward Artificial Cell‐Mediated Tissue Engineering: A New Perspective

Toward Artificial Cell‐Mediated Tissue Engineering: A New Perspective

Deciphering the underlying mechanism of liver diseases through utilization of multicellular hepatic spheroid models

Nonhuman primate models of pediatric viral diseases

Contact Info

Product

Resources

About