3D image reconstruction of transparent microscopic objects using digital holography

Holography: The Usefulness of Digital Holographic Microscopy for Clinical Diagnostics

“…The reconstructed image contains information about the entire depth of the field of view. For reconstruction to be accurate, the sample has to be transparent and homogenous, where the differences in refractive index between the background and the cells create the tomography of the image [19].…”

Rolling Shutter Effect aberration compensation in Digital Holographic Microscopy

“…In this regard, it may represent a major obstacle in interferometry techniques. Although the aim of this paper is not an exhaustive study of how one type of device differs from another, we will focus on some properties that are sensitive to a particular application such as the Digital Holographic Microscopy (DHM) [1,[5][6][7][8][9]. In the literature, several works report solutions for eliminating or mitigating RSE by using either mechanical or electrical devices or by mathematical algorithms that generally require multiple images of the same scene for synchronization [2,10].…”

Alternative Reconstruction Method and Object Analysis in Digital Holographic Microscopy

“…DHM is a powerful technique for real-time quantitative phase contrast imaging, since a single intensity image, called a hologram, allows the reconstruction of the phase shift induced by a specimen. This property of holograms offers phase-contrast techniques, which can then be used for quantitative 3D imaging (Palacios et al, 2005). Quantitative phase imaging is important because it allows the determination of the optical thickness profile of a transparent object with sub-wavelength accuracy (Yu et al, 2009).…”