3D in vitro corneal models: A review of current technologies

Shiju, Thomas Michael; Oliveira, Rodrigo Carlos de; Wilson, Steven E.

doi:10.1016/j.exer.2020.108213

Cited by 30 publications

(32 citation statements)

References 76 publications

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“…The emergence of three-dimensional (3D) printing technology has provided researchers with numerous strategies for creating prosthetic organs or tissues that are biocompatible, degradable and functional [17]. In recent years, 3D printing has demonstrated its potential in regenerative medicine, being used to create patient-specific beating hearts, distensile airways and central nervous system scaffolds [18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Three-dimensional bioprinting of artificial ovaries by an extrusion-based method using gelatin-methacryloyl bioink

Gao

et al. 2021

Climacteric

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Purpose: The aim of this study was to design and fabricate a three-dimensional (3D) printed artificial ovary. Methods: We first compared the printability of gelatin-methacryloyl (GelMA), alginate and GelMA-alginate bioinks, of which GelMA was selected for further investigation. The swelling properties, degradation kinetics and shape fidelity of GelMA scaffolds were characterized by equilibrium swelling/ lyophilization, collagenase processing and micro-computed tomography evaluation. Commercial ovarian tumor cell lines (COV434, KGN, ID8) and primary culture ovarian somatic cells were utilized to perform cell-laden 3D printing, and the results were evaluated by live/dead assays and TUNEL detection. Murine ovarian follicles were seeded in the ovarian scaffold and their diameters were recorded every day. Finally, in vitro maturation was performed, and the ovulated oocytes were collected and observed. Results: Our results indicated that GelMA was suitable for 3D printing fabrication. Its scaffolds performed well in terms of hygroscopicity, degradation kinetics and shape fidelity. The viability of ovarian somatic cells was lower than that of commercial cell lines, suggesting that extrusion-based 3D culture fabrication is not suitable for primary ovarian cells. Nevertheless, the GelMA-based 3D printing system provided an appropriate microenvironment for ovarian follicles, which successfully grew and ovulated in the scaffolds. Metaphase II oocytes were also observed after in vitro maturation. Conclusions: The GelMA-based 3D printing culture system is a viable alternative option for follicular growth, development and transfer. Accordingly, it shows promise for clinical application in the treatment of female endocrine and reproductive conditions.

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Section: Introductionmentioning

confidence: 99%

Three-dimensional bioprinting of artificial ovaries by an extrusion-based method using gelatin-methacryloyl bioink

Gao

et al. 2021

Climacteric

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“…However, any damage in the basal area would lead to a dysfunction of the LSCs that would result in overgrowth of the conjunctiva and blood vessels, photophobia and pain [32]. The Bowman's membrane is a thin acellular layer that separates the epithelium from the stroma [31]. The stroma is the widest part of the cornea.…”

Section: Corneamentioning

confidence: 99%

“…This structural complexity is the key to its transparency and mechanical resistance [33,34]. In addition, it is composed of keratinocyte cells, which are responsible for maintaining the ECM [31]. These cells have little mitotic activity and present dendritic morphology.…”

Section: Corneamentioning

confidence: 99%

“…Nevertheless, in case of trauma, they are activated as a fibroblast that can differentiate into myofibroblasts [34,35]. Myofibroblasts express proteins that can alter the ECM causing opacity of the cornea, contraction and a corneal scar formation [31,35]. Descement's membrane is an acellular layer that separates the stroma from the endothelium [31].…”

Section: Corneamentioning

confidence: 99%

“…Myofibroblasts express proteins that can alter the ECM causing opacity of the cornea, contraction and a corneal scar formation [31,35]. Descement's membrane is an acellular layer that separates the stroma from the endothelium [31]. The endothelium is the deepest layer and is composed of endothelial cells that are responsible for maintaining the fluid balance of the cornea [31].…”

Section: Corneamentioning

confidence: 99%

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Current Insights into 3D Bioprinting: An Advanced Approach for Eye Tissue Regeneration

et al. 2021

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Three-dimensional (3D) printing is a game changer technology that holds great promise for a wide variety of biomedical applications, including ophthalmology. Through this emerging technique, specific eye tissues can be custom-fabricated in a flexible and automated way, incorporating different cell types and biomaterials in precise anatomical 3D geometries. However, and despite the great progress and possibilities generated in recent years, there are still challenges to overcome that jeopardize its clinical application in regular practice. The main goal of this review is to provide an in-depth understanding of the current status and implementation of 3D bioprinting technology in the ophthalmology field in order to manufacture relevant tissues such as cornea, retina and conjunctiva. Special attention is paid to the description of the most commonly employed bioprinting methods, and the most relevant eye tissue engineering studies performed by 3D bioprinting technology at preclinical level. In addition, other relevant issues related to use of 3D bioprinting for ocular drug delivery, as well as both ethical and regulatory aspects, are analyzed. Through this review, we aim to raise awareness among the research community and report recent advances and future directions in order to apply this advanced therapy in the eye tissue regeneration field.

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Advancements in Three Dimensional In‐Vitro Cell Culture Models

Singh

2022

The Chemical Record

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The scientific field is observing a gradual shift from monolayer cultures to threedimensional (3D) models, as they give a more relevant data in pre-clinical stages. This review summarizes the major techniques and materials used to develop 3D platforms, especially for cancer. It also discusses the challenges and some unresolved issues of the field and highlights some techniques that have made it to the market.

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3D in vitro corneal models: A review of current technologies

Cited by 30 publications

References 76 publications

Three-dimensional bioprinting of artificial ovaries by an extrusion-based method using gelatin-methacryloyl bioink

Three-dimensional bioprinting of artificial ovaries by an extrusion-based method using gelatin-methacryloyl bioink

Current Insights into 3D Bioprinting: An Advanced Approach for Eye Tissue Regeneration

Advancements in Three Dimensional In‐Vitro Cell Culture Models

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