3D in vivo imaging of the keratin filament network in the mouse stratum granulosum reveals profilaggrin-dependent regulation of keratin bundling

Usui, Keiko; Kadono, Nanako; Furuichi, Yuki; Shiraga, Keiichiro; Saitou, Takashi; Kawasaki, Hiroshi; Toyooka, Kiminori; Tamura, Hiroomi; Kubo, Akiharu; Amagai, Masayuki; Matsui, Toshimitsu

doi:10.1016/j.jdermsci.2019.04.006

Cited by 4 publications

(4 citation statements)

References 10 publications

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“…We surmise that KG-interacting keratin fibers further pack and bundle as growing arrays of KGs exclude a larger volume of the cytoplasm. In line with this view, keratin network defects are already evident in the granular layer of Flg -null mice ( Kawasaki et al., 2012 ; Usui et al., 2019 ). The collective evidence points to a role for growing KGs in orchestrating progressive keratin assembly before FLG processing and corneoptosis.…”

Section: Toward Cracking the Skin Barriersupporting

confidence: 64%

“…3c) (Quiroz et al, 2020). Through electron microscopy, these keratin-caged KGs appear prominently on horizontal, en face sections of mouse epidermis (Usui et al, 2019). Furthermore, this restriction of KG fusion by K1/K10 fibers likely controls KG size, as suggested by enlarged KGs upon genetic ablation of krt10 in mice (Kumar et al, 2015).…”

Section: Liquid-liquid Phase Separation Dynamics Through Skin Barrier Formationmentioning

confidence: 97%

“…Departing from the extensive liquid-like fusion of de novo assembled KGs in cultured KCs, abundant in vivo keratin-1/keratin-10 (K1/K10) fibers interact with FLG on the surface of native KGs to cage them, preventing KG fusion ( Figure 3 c) ( Quiroz et al., 2020 ). Through electron microscopy, these keratin-caged KGs appear prominently on horizontal, en face sections of mouse epidermis ( Usui et al., 2019 ). Furthermore, this restriction of KG fusion by K1/K10 fibers likely controls KG size, as suggested by enlarged KGs on genetic ablation of K10 in mice ( Kumar et al., 2015 ).…”

Section: Toward Cracking the Skin Barriermentioning

confidence: 99%

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Cracking the Skin Barrier: Liquid-Liquid Phase Separation Shines under the Skin

Avecilla

Quiroz

2021

JID Innovations

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Toward Cracking the Skin Barriersupporting

confidence: 64%

Section: Liquid-liquid Phase Separation Dynamics Through Skin Barrier Formationmentioning

confidence: 97%

Section: Toward Cracking the Skin Barriermentioning

confidence: 99%

See 1 more Smart Citation

Cracking the Skin Barrier: Liquid-Liquid Phase Separation Shines under the Skin

Avecilla

Quiroz

2021

JID Innovations

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“…Daughter keratinocytes first enter the stratum spinosum, forming a layer of polyhedral-shaped cells joined together by desmosomes, intracellular junctions that mediate cell–cell adhesion and reinforce the epidermis against physical trauma [ 14 ]. Above the stratum spinosum is the stratum granulosum, a layer of flattened keratinocytes that form cytoplasmic keratohyalin granules to crosslink keratin filaments and create the water-impermeable barrier [ 15 ]. Finally, keratinocytes enter the external tier of the epidermis, the stratum corneum, where they release lysosomal enzymes that degrade their intracellular components [ 16 ].…”

Section: The Skin Barriermentioning

confidence: 99%

The Skin Microbiome: Current Landscape and Future Opportunities

Smythe

Wilkinson

2023

IJMS

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Our skin is the largest organ of the body, serving as an important barrier against the harsh extrinsic environment. Alongside preventing desiccation, chemical damage and hypothermia, this barrier protects the body from invading pathogens through a sophisticated innate immune response and co-adapted consortium of commensal microorganisms, collectively termed the microbiota. These microorganisms inhabit distinct biogeographical regions dictated by skin physiology. Thus, it follows that perturbations to normal skin homeostasis, as occurs with ageing, diabetes and skin disease, can cause microbial dysbiosis and increase infection risk. In this review, we discuss emerging concepts in skin microbiome research, highlighting pertinent links between skin ageing, the microbiome and cutaneous repair. Moreover, we address gaps in current knowledge and highlight key areas requiring further exploration. Future advances in this field could revolutionise the way we treat microbial dysbiosis associated with skin ageing and other pathologies.

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