3D interferometric optical tweezers using a single spatial light modulator

Schonbrun, Ethan; Piestun, Rafael; Jordan, Pamela; Cooper, Jonathan M.; Wulff, Kurt D.; Courtial, Johannes; Padgett, Miles J.

doi:10.1364/opex.13.003777

Cited by 113 publications

(55 citation statements)

References 20 publications

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“…We address the SLM to produce a kspace distribution of paraxial plane waves [13]. The plane of the SLM is imaged to a CCD detector where the interference patterns are recorded.…”

Section: Introductionmentioning

confidence: 99%

Topology of optical vortex lines formed by the interference of three, four, and five plane waves

O’Holleran¹,

Padgett²,

Dennis³

2006

Opt. Express

148

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Abstract:When three or more plane waves overlap in space, complete destructive interference occurs on nodal lines, also called phase singularities or optical vortices. For super positions of three plane waves, the vortices are straight, parallel lines. For four plane waves the vortices form an array of closed or open loops. For five or more plane waves the loops are irregular. We illustrate these patterns numerically and experimentally and explain the three-, four-and five-wave topologies with a phasor argument.

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“…We address the SLM to produce a kspace distribution of paraxial plane waves [13]. The plane of the SLM is imaged to a CCD detector where the interference patterns are recorded.…”

Section: Introductionmentioning

confidence: 99%

Topology of optical vortex lines formed by the interference of three, four, and five plane waves

O’Holleran¹,

Padgett²,

Dennis³

2006

Opt. Express

148

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“…Multiple beam paths can be created by splitting a single beam of light into more beams and forming a spatial pattern. Spatial light modulators are instruments specifically designed to achieve dynamic trapping patterns [150]. Both holographic optical tweezers and generalized phase contrast based optical traps use spatial light modulator technology to create reconfigurable trap sites in 3D space.…”

Section: Applicationmentioning

confidence: 99%

Microfluidic bio-particle manipulation for biotechnology

Çetin

Özer

Solmaz

2014

Biochemical Engineering Journal

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a b s t r a c tMicrofluidics and lab-on-a-chip technology offers unique advantages for the next generation devices for diagnostic therapeutic applications. For chemical, biological and biomedical analysis in microfluidic systems, there are some fundamental operations such as separation, focusing, filtering, concentration, trapping, detection, sorting, counting, washing, lysis of bio-particles, and PCR-like reactions. The combination of these operations led to the complete analysis systems for specific applications. Manipulation of the bio-particles is the key ingredient for these applications. Therefore, microfluidic bio-particle manipulation has attracted a significant attention from the academic community. Considering the size of the bio-particles and the throughput of the practical applications, manipulation of the bio-particles is a challenging problem. Different techniques are available for the manipulation of bio-particles in microfluidic systems. In this review, some of the techniques for the manipulation of bio-particles; namely hydrodynamic based, electrokinetic-based, acoustic-based, magnetic-based and optical-based methods have been discussed. The comparison of different techniques and the recent applications regarding the microfluidic bio-particle manipulation for different biotechnology applications are presented. Finally, challenges and the future research directions for microfluidic bio-particle manipulation are addressed.

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“…In microscopy these devices have been used extensively in STED (Gould et al, 2012;Lenz et al, 2011), holographic optical tweezers (Schonbrun et al, 2005), adaptive optics (Neil et al, 2000b), structured illumination (Débarre et al, 2008;Kner et al, 2009) and pulse shaping (Weiner, 2000).…”

Section: Programmable Beamlet Generation Using a Spatial Light Modulatormentioning

confidence: 99%

Multifocal multiphoton microscopy with adaptive optical correction

et al. 2013

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Multiphoton microscopy (MPM) is a remarkably versatile technique in biologicalimaging. MPM provides increased depth over confocal imaging and can be combined with other imaging techniques such as fluorescence lifetime imaging microscopy (FLIM), adding functional information. FLIM read--out is relatively straightforward using time--correlated single photon counting (TCSPC). Fluorescence lifetime detection enhances the power of multiphoton imaging to allow three dimensional, concentration independent, measurements of environmental parameters such as pH, Oxygen tension and Ca 2+ in addtion to the interaction or conformational modification of proteins by Förster resonant energy transfer (FRET); the latter is a particular focus of the Dimbleby research groups at King's CollegeLondon. However, there are significant limitations in both FLIM and MM.Limitations of TCSPC--FLIM include prolonged acquisition times along with signal and resolution degradation as a function of depth. This thesis demonstrates advancements multiphoton fluorescence lifetime imaging through improvements in two principal areas: speed and resolution at depth.In order to improve acquistion rates a multifocal multiphoton microscope (MMM) capable of rapid, parallelized TCSPC--FLIM was developed --MegaFLI. Acquisitions demonstrate rapid 3--dimensional, high temporal resolution FLIM in vivo Zebrafish.Performed by massively parallel excitation/detection the speed is signficantly improved by a factor of 64.In parallel to the MegaFLI project, a second microscope employing adaptive optical correction has been developed. The introduction of Adaptive Optics (AO) serves to improve imaging quality by counteracting the refractive index heterogeneities introduced by the sample, limiting the imaging depth. Incorporated with a single beam scanning FLIM system, a pupil--segmentation AO--TCSPC--FLIM demonstrates improved signal--to--noise ratio (SNR) and resolution, permiting a more accurate determination of fluorescent lifetime in turbid media. 3 DECLARATION OF AUTHORSHIPThe work present is my own work with the exception of TRI2, which was developed The introduction (Chapter 1) concentrates on optical methods developed in the context of multiphoton microscopy (MPM) or more specifically two--photon microscopy (TPM) (Denk et al., 1990). TPM enables long--term imaging of in vivo biological specimens, image generation at increased tissue depth, and higher signal--to--noise images compared to wide--field and confocal approaches (Helmchen and In comparison to confocal microscopy, two--photon microscopy (TPM) offers considerable benefits since excitation is confined to a femtolitre volume in the vicinity of the focal plane, reducing overall photobleaching and phototoxicity of thick samples (Centonze and White, 1998;Denk et al., 1990;Helmchen and Denk, 2005;Williams et al., 2001). Out--of--focus excitation is avoided due to the localization of the excitation making the confocal pinhole obsolete (Amos and Where is the numerical aperture of the obj...

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3D interferometric optical tweezers using a single spatial light modulator

Cited by 113 publications

References 20 publications

Topology of optical vortex lines formed by the interference of three, four, and five plane waves

Topology of optical vortex lines formed by the interference of three, four, and five plane waves

Microfluidic bio-particle manipulation for biotechnology

Multifocal multiphoton microscopy with adaptive optical correction

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