3D mapping of native extracellular matrix reveals cellular responses to the microenvironment

Lansky, Zipora; Mutsafi, Yael; Houben, Lothar; Ilani, Tal; Armony, Gad; Wolf, Sharon G.; Fass, Deborah

doi:10.1016/j.yjsbx.2018.100002

Cited by 23 publications

(29 citation statements)

References 70 publications

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“…In addition to providing an anatomical basis for tonotopic maps and frequency tuning, the BM may interact with the cellular responses of the OC sensory and supporting cells, with which it in close contact, thereby further influencing cochlear responses beyond alterations in the tonotopicity of the BM 66 , 67 . Macrophages located within the BM activate in response to sensory cell damage during acute stress (e.g.…”

Section: Discussionmentioning

confidence: 99%

Postnatal structural development of mammalian Basilar Membrane provides anatomical basis for the maturation of tonotopic maps and frequency tuning

Tani

Koike-Tani

Tran

et al. 2021

Sci Rep

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The basilar membrane (BM) of the mammalian cochlea constitutes a spiraling acellular ribbon that is intimately attached to the organ of Corti. Its graded stiffness, increasing from apex to the base of the cochlea provides the mechanical basis for sound frequency analysis. Despite its central role in auditory signal transduction, virtually nothing is known about the BM’s structural development. Using polarized light microscopy, the present study characterized the architectural transformations of freshly dissected BM at time points during postnatal development and maturation. The results indicate that the BM structural elements increase progressively in size, becoming radially aligned and more tightly packed with maturation and reach the adult structural signature by postnatal day 20 (P20). The findings provide insight into structural details and developmental changes of the mammalian BM, suggesting that BM is a dynamic structure that changes throughout the life of an animal.

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Section: Discussionmentioning

confidence: 99%

Postnatal structural development of mammalian Basilar Membrane provides anatomical basis for the maturation of tonotopic maps and frequency tuning

Tani

Koike-Tani

Tran

et al. 2021

Sci Rep

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“…For the characterization of the functionalization (more details in the Supplementary Materials), a six-channel spectroscopic SPR sensor developed at the Institute of Photonics and Electronics (Prague, Czech Republic) combined with a dispersionless microfluidic system equipped with temperature controller was used. For this sensor at a resonant wavelength around 750 nm, a 1-nm SPR wavelength shift represents a change in the surface protein concentration of 17 ng/cm 2 [2]. Antifouling properties were confirmed using an angular MP-SPR with a 4-channel microfluidic system, equipped with LED sources of 670 nm and 785 nm wavelengths (BioNavis 400 KONTIO, BioNavis, Tempere, Finland).…”

Section: Surface Characterization By Sprmentioning

confidence: 99%

“…Living cells are continuously sensing their microenvironment and adapt to different physicochemical events during their lifetime [1,2]. Besides soluble chemical signals, mechanical cues influence cells persistently over time, involving the activation or inhibition of different signaling pathways [3].…”

Section: Introductionmentioning

confidence: 99%

Functionalizable Antifouling Coatings as Tunable Platforms for the Stress-Driven Manipulation of Living Cell Machinery

et al. 2020

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Cells are continuously sensing their microenvironment and subsequently respond to different physicochemical cues by the activation or inhibition of different signaling pathways. To study a very complex cellular response, it is necessary to diminish background environmental influences and highlight the particular event. However, surface-driven nonspecific interactions of the abundant biomolecules from the environment influence the targeted cell response significantly. Yes-associated protein (YAP) translocation may serve as a marker of human hepatocellular carcinoma (Huh7) cell responses to the extracellular matrix and surface-mediated stresses. Here, we propose a platform of tunable functionable antifouling poly(carboxybetain) (pCB)-based brushes to achieve a molecularly clean background for studying arginine, glycine, and aspartic acid (RGD)-induced YAP-connected mechanotransduction. Using two different sets of RGD-functionalized zwitterionic antifouling coatings with varying compositions of the antifouling layer, a clear correlation of YAP distribution with RGD functionalization concentrations was observed. On the other hand, commonly used surface passivation by the oligo(ethylene glycol)-based self-assembled monolayer (SAM) shows no potential to induce dependency of the YAP distribution on RGD concentrations. The results indicate that the antifouling background is a crucial component of surface-based cellular response studies, and pCB-based zwitterionic antifouling brush architectures may serve as a potential next-generation easily functionable surface platform for the monitoring and quantification of cellular processes.

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“…2) [8], he talked about the privilege of being able to study whatever interested him, and he touched on some of his more unusual projects, including a decelerator for CCD cameras [9], polymer studies for energy applications [7], and more. I will present previous results [10,11] and new studies of mineral storage deposits in the mitochondria of mammalian and other eukaryotic cells, by cryoSTEM tomography (CSTET) [12,13] and on-the-spot chemical characterization by EDX. STEM imaging mode allows for quantification of elemental content in intact cells [14,15], and it is fundamental to development of aloof spectroscopy techniques [16,17], which hold great promise for biological materials.…”

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confidence: 99%

Taking the Road Less Travelled – the Downing Legacy

Wolf

2019

Microsc Microanal

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3D mapping of native extracellular matrix reveals cellular responses to the microenvironment

Cited by 23 publications

References 70 publications

Postnatal structural development of mammalian Basilar Membrane provides anatomical basis for the maturation of tonotopic maps and frequency tuning

Postnatal structural development of mammalian Basilar Membrane provides anatomical basis for the maturation of tonotopic maps and frequency tuning

Functionalizable Antifouling Coatings as Tunable Platforms for the Stress-Driven Manipulation of Living Cell Machinery

Taking the Road Less Travelled – the Downing Legacy

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