3D matrixed DNA self-nanocatalyzer as electrochemical sensitizers for ultrasensitive investigation of DNA 5-methylcytosine

Yang, Haoyang; Luo, Jing; Huang, Hui; Fang, Lichao; Deng, Jun; Li, Chenghong; Li, Yan; Zeng, Tao; Zheng, Junsong

doi:10.1016/j.aca.2020.10.064

Cited by 9 publications

(6 citation statements)

References 28 publications

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“…198 Metal nanostructures are of great importance in designing electrodes, where the conductivity of metal NPs results in higher sensitivity of the detection system. 199 For example, Zhu et al 200 fabricated a biomimetic Au-based DNA self-nano catalyzer called AuNPs/g-C 3 N 4 − rGO for electrochemical sensing of DNA 5-methylcytosine, which is related to methylation-related diseases. The sensing principle is illustrated in Figure 3a.…”

Section: Biomedical Applications Of Biometalmentioning

confidence: 99%

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Biomimetic Metallic Nanostructures for Biomedical Applications, Catalysis, and Beyond

Yaraki

Nasab

Zare³

et al. 2022

Ind. Eng. Chem. Res.

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Nature has inspired scientists to develop green and sustainable nanomaterials with biomimetic functions. Particularly, biomimetic metallic nanostructures (biometal NPs) with unique optical, catalytic, and electrical properties have received tremendous attention in many fields, ranging from healthcare and agriculture to energy and environmental sciences. Biometal NPs synthesized by various natural resources such as plant extracts, biomolecules, bacteria, and even viruses possess unique biomimetic functions including but not limited to precise biorecognition, selfassembly, antibacterial/antiviral, and enzymatic properties. In this report, we first review the bioinspired synthesis of industrially important metal nanoparticles, followed by the discussion on how the different biological sources affect the biomimetic functions of the as-synthesized biometal NPs. Next, we review the recent advancement and applications of these biometal NPs in the fields of biomedical engineering and catalysis, which include the development of metallic nanobiosensors, biomedical imaging probes, nanotherapeutics (e.g., antimicrobial and photodynamic/photothermal therapeutic agents), as well as the design of multifunctional nanozymes and artificial metalloenzymes for chemical and biopharmaceutical industries. Finally, we highlight some of the latest advancements in nanobiomimicry and their emerging applications in clean energy, electronic devices, and data storage, which shows the game-changing role of biomimetic metallic nanostructures for various technological applications in the near future.

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Section: Biomedical Applications Of Biometalmentioning

confidence: 99%

“…Schematic illustration of (a) (i) synthesis of 3D matrix DNA self-nanocatalyzer and (ii) design principle of Au-based DNA self-nano catalyst for electrochemical detection of DNA 5-methylcytosine. Reproduced with permission from ref . Copyright 2021 Elsevier.…”

Section: Biomedical Applications Of Biometal Nanoparticlesmentioning

confidence: 99%

Biomimetic Metallic Nanostructures for Biomedical Applications, Catalysis, and Beyond

Yaraki

Nasab

Zare³

et al. 2022

Ind. Eng. Chem. Res.

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“…To accelerate the uptake of DNA methylation technologies into the diagnostic laboratory setting, developments of cost-effective, rapid, simple, and easy-to-interpret DNA methylation methods for point-of-care diagnostics are required. In this regard, electrochemical/electrochemiluminescence (ECL)-based methods have been reported for detection of 5-methylcytosine (5mC). − Despite their generally advanced detection strategies and improved detection limits, these methods are made using external electronic readers, which require a signal converter and data processing unit . Moreover, these strategies generally suffer from some limitations, including high potential oxidation of mC with the possibility of overlapping of the analytical signal with the oxidation signal of a thymine, complicated labeling procedures, and susceptibility to degradation and denaturation of enzyme-based methods.…”

mentioning

confidence: 99%

“…Therefore, low abundance determination of 5-mC DNA methylation makes their determination very challenging. Some electrochemical signal amplification strategies for DNA methylation detection have been established in response to this demand, including enzyme-based rolling circle amplification (RCA), labeling of a single antibody with multiple HRPs, enzyme-free supersandwich DNA structure-based assembly, and a 3D matrixed DNA self-nanocatalyzer via gold nanoparticles (AuNPs) . Changing the conformation of the enzyme during the experiment will influence the bioactivity of the enzymes in enzyme-based methods.…”

mentioning

confidence: 99%

“…Some electrochemical signal amplification strategies for DNA methylation detection have been established in response to this demand, including enzyme-based rolling circle amplification (RCA), 33 labeling of a single antibody with multiple HRPs, 17 enzyme-free supersandwich DNA structurebased assembly, 23 and a 3D matrixed DNA self-nanocatalyzer via gold nanoparticles (AuNPs). 18 Changing the conformation of the enzyme during the experiment will influence the bioactivity of the enzymes in enzyme-based methods. Furthermore, inherent problems with enzyme-free systems, such as high cost, time-consuming, and stringent experimental conditions, limit their use in clinical laboratories and as pointof-care devices.…”

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confidence: 99%

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Smartphone-Based Electrochemiluminescence for Visual Simultaneous Detection of RASSF1A and SLC5A8 Tumor Suppressor Gene Methylation in Thyroid Cancer Patient Plasma

et al. 2022

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Visual one-step simultaneous detection of low-abundance methylation is a crucial challenge in early cancer diagnosis in a simple manner. Through the design of a closed split bipolar electrochemistry system (BE), detection of promoter methylation of tumor suppressor genes in papillary thyroid cancer, RASSF1A and SLC5A8, was achieved using electrochemiluminescence. For this purpose, electrochemiluminescence of luminol loaded into the Fe 3 O 4 @UiO-66 and gold nanorod-functionalized graphite-like carbon nitride nanosheet (AuNRs@C 3 N 4 NS), separately, on the anodic and cathodic pole bipolar electrodes (BPEs) in two different chambers of a bipolar cell were recorded on a smartphone camera. To provide the same electric potential (ΔE elec ) through the BPEs to conduct simultaneous light emission, as well as to achieve higher sensitivity, anodic and cathodic poles BPEs were separately connected to ruthenium nanoparticles electrodeposited on nitrogen-doped graphene-coated Cu foam (fCu/N-GN/RuNPs) to provide a hydrogen evolution reaction (HER) and polycatechol-modified reduced graphene oxide/pencil graphite electrode (PC-rGO/PGE) to provide electrooxidation of hydrazine. Moreover, taking advantages of the strong cathodic ECL activity due to the roles of AuNRs, as well as the high density of capture probes on the UiO-66 and Fe 3 O 4 roles in improving the signal-to-background ratio (S/B) in complicated plasma media, a sensitive visual ECL immunosensor was developed to detect two different genes as model target analytes in patient plasma samples. The ability of discrimination of methylation levels as low as 0.01% and above 90% clinical sensitivity in thyroid cancer patient plasma implies that the present strategy is able to diagnose cancer early, as well as monitor responses of patients to therapeutic agents.

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End-labeling-based electrochemical strategy for detection of adenine methylation in nucleic acid by differential pulse voltammetry

et al. 2021

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3D matrixed DNA self-nanocatalyzer as electrochemical sensitizers for ultrasensitive investigation of DNA 5-methylcytosine

Cited by 9 publications

References 28 publications

Biomimetic Metallic Nanostructures for Biomedical Applications, Catalysis, and Beyond

Biomimetic Metallic Nanostructures for Biomedical Applications, Catalysis, and Beyond

Smartphone-Based Electrochemiluminescence for Visual Simultaneous Detection of RASSF1A and SLC5A8 Tumor Suppressor Gene Methylation in Thyroid Cancer Patient Plasma

End-labeling-based electrochemical strategy for detection of adenine methylation in nucleic acid by differential pulse voltammetry

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