3D Microfibrous Scaffolds Selectively Promotes Proliferation and Glial Differentiation of Adult Neural Stem Cells: A Platform to Tune Cellular Behavior in Neural Tissue Engineering

Patel, Bhavika B.; Sharifi, Farrokh; Stroud, Daniel P.; Montazami, Reza; Hashemi, Nastaran; Sakaguchi, Donald S.

doi:10.1002/mabi.201970003

Cited by 11 publications

(14 citation statements)

References 49 publications

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“…On the other hand, scaffolds made from biocompatible materials may provide structural support for cell adhesion, proliferation, and differentiation mimicking the cellular microenvironment. A number of scaffolds made from solid [ 3 , 4 , 5 ], microfibrous materials [ 6 ], or gels [ 7 ] have been previously described to support the proliferation and localized differentiation of murine neural stem cells, indicating their potential value for neural tissue regeneration [ 8 ]. Among them, 3D foam biomaterials provide efficient cell adhesion, proliferation, and differentiation due to their unique properties (e.g., high surface-to-volume ratio, 3D porous structure) [ 9 ].…”

Section: Introductionmentioning

confidence: 99%

Biocompatibility of α-Al2O3 Ceramic Substrates with Human Neural Precursor Cells

Asimakopoulou

Gkekas

Kastrinaki

et al. 2020

JFB

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Background: Biocompatible materials-topography could be used for the construction of scaffolds allowing the three-dimensional (3D) organization of human stem cells into functional tissue-like structures with a defined architecture. Methods: Structural characterization of an alumina-based substrate was performed through XRD, Brunauer–Emmett–Teller (BET) analysis, scanning electron microscopy (SEM), and wettability measurements. Biocompatibility of the substrate was assessed by measuring the proliferation and differentiation of human neural precursor stem cells (NPCs). Results: α-Al2O3 is a ceramic material with crystallite size of 40 nm; its surface consists of aggregates in the range of 8–22 μm which forms a rough surface in the microscale with 1–8 μm cavities. The non-calcined material has a surface area of 5.5 m2/gr and pore size distribution of 20 nm, which is eliminated in the calcined structure. Thus, the pore network on the surface and the body of the ceramic becomes more water proof, as indicated by wettability measurements. The alumina-based substrate supported the proliferation of human NPCs and their differentiation into functional neurons. Conclusions: Our work indicates the potential use of alumina for the construction of 3D engineered biosystems utilizing human neurons. Such systems may be useful for diagnostic purposes, drug testing, or biotechnological applications.

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Section: Introductionmentioning

confidence: 99%

Biocompatibility of α-Al2O3 Ceramic Substrates with Human Neural Precursor Cells

Asimakopoulou

Gkekas

Kastrinaki

et al. 2020

JFB

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“…Highly tunable surface topography for hydrogel microfibers will be of particular interest in research endeavors where cells are being seeded onto the surface of microfibers, since surface topography is known to affect cell proliferation, differentiation, and overall health [3,6,7]. Cells are greatly affected by their microenvironment, and this simple method to modify microfiber surface topography can be utilized to provide an additional degree of control over the behavior of cells, adding another level of control in a powerful manufacturing process to create continuous and tunable hydrogel microfibers.…”

Section: Discussionmentioning

confidence: 99%

“…Their favorable attributes stem from their high surface-to-area ratio, rapid diffusion gradients and strong potential for biocompatibility [1][2][3]. Their contributions to biomedical fields thus far have included the encapsulation or seeding of bioactive molecules [4,5], cells [1,[6][7][8], or bacteria [9]; furthermore, their properties allow for the creation of a microenvironment that mimics conditions within the body, providing an extracellular-matrix (ECM) inspired scaffold for arranging and guiding cell growth, proliferation and differentiation [1,3,6,7].…”

Section: Introductionmentioning

confidence: 99%

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Shear at Fluid-Fluid Interfaces Affects the Surface Topologies of Alginate Microfibers

et al. 2019

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Hydrogel microfibers have great potential for applications such as tissue engineering or three-dimensional cell culturing. Their favorable attributes can lead to tissue models that can help to reduce or eliminate animal testing, thereby providing an eco-friendly alternative to this unsustainable process. In addition to their highly tunable mechanical properties, this study shows that varying the viscosity and flow rates of the prepolymer core solution and gellator sheath solution within a microfluidic device can affect the surface topology of the resulting microfibers. Higher viscosity core solutions are more resistant to deformation from shear force within the microfluidic device, thereby yielding smoother fibers. Similarly, maintaining a smaller velocity gradient between the fluids within the microfluidic device minimizes shear force and smooths fiber surfaces. This simple modification provides insight into manufacturing microfibers with highly tunable properties.

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“…In vivo results demonstrated that this hydrogel promotes SCI mouseendogenous neurogenesis and functional recovery by restoring interrupted spinal cord circuits. PCL can mimic the structure of gray or white matter and promote the differentiation of NSCs to oligodendrocytes and the formation of axonal myelin sheaths, it is an ideal material in SCI tissue engineering (Donoghue et al, 2013;Patel et al, 2019). Wong et al (2008) implanted PCL into an animal model of total transection of SCI, and its open microchannel structure facilitates axon regeneration and myelination.…”

Section: Hydrogel Therapy Alonementioning

confidence: 99%

Hydrogels in Spinal Cord Injury Repair: A Review

Dong

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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Traffic accidents and falling objects are responsible for most spinal cord injuries (SCIs). SCI is characterized by high disability and tends to occur among the young, seriously affecting patients’ lives and quality of life. The key aims of repairing SCI include preventing secondary nerve injury, inhibiting glial scarring and inflammatory response, and promoting nerve regeneration. Hydrogels have good biocompatibility and degradability, low immunogenicity, and easy-to-adjust mechanical properties. While providing structural scaffolds for tissues, hydrogels can also be used as slow-release carriers in neural tissue engineering to promote cell proliferation, migration, and differentiation, as well as accelerate the repair of damaged tissue. This review discusses the characteristics of hydrogels and their advantages as delivery vehicles, as well as expounds on the progress made in hydrogel therapy (alone or combined with cells and molecules) to repair SCI. In addition, we discuss the prospects of hydrogels in clinical research and provide new ideas for the treatment of SCI.

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3D Microfibrous Scaffolds Selectively Promotes Proliferation and Glial Differentiation of Adult Neural Stem Cells: A Platform to Tune Cellular Behavior in Neural Tissue Engineering

Cited by 11 publications

References 49 publications

Biocompatibility of α-Al2O3 Ceramic Substrates with Human Neural Precursor Cells

Biocompatibility of α-Al2O3 Ceramic Substrates with Human Neural Precursor Cells

Shear at Fluid-Fluid Interfaces Affects the Surface Topologies of Alginate Microfibers

Hydrogels in Spinal Cord Injury Repair: A Review

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