3D osteocyte lacunar morphometric properties and distributions in human femoral cortical bone using synchrotron radiation micro-CT images

Long bone maturation is driven by pore closing: A quantitative tomography investigation of structural formation in young C57BL/6 mice

“…Small shifts to this threshold value thus lead to 632 negligible effects on the BV parameter however they lead to signifi-633 cant changes in the closed porosity estimates, because the closed 634 pore volume distribution-range is comparatively very small.However, we note that the results that we obtain for lab-based 636 lCT measurements fall within ranges reported for closed porosity 637 in small mammals observed by other authors[25,26,36,38]. Also, 638 the closed porosity for our adult (182 day old) samples measured 639 using a different imaging contrast: synchrotron radiation phase-640 contrast enhancement[39,40] revealed a mean value of 641 1.4 ± 0.04% which matches other reports [25,26] of $1.3 closed 642 porosity % for adult C57BL/6 femora. Our observations showing 643 comparable sizes by histological 2D and tomographic data (see…”

“…39 During a shaping phase (III), the bones gradually attain their characteristic intricate adult form. 40 Architectured mineral depositioning -first in open foamy scaffolds, and later into solid bone material 41 -is presumably a compromise between the mechanical needs of providing support to the body, and 42 the biological requirements of vascularization and extensive nutritional needs in the early stages of bone 43 formation. midshaft cross section of C57BL/6 mice becomes elliptical between 83 7 and 8 weeks of age and that during growth, woven and lamellar 84 bone types are present.…”

Quantification of the 3d Morphology of the Bone Cell Network From Synchrotron Micro-Ct Images

“…In the segmented osteocyte lacunae image, unacceptable objects such as micro-cracks, ring artifacts, and some irregularly shaped fragments can be extracted as well. From the literature, it is known that the size and shape of lacunae resides in a certain range (Mc-Creadie et al, 2004;Dong et al, 2014). Thus, the segmented lacunae were filtered by thresholding some descriptors as follows: 50 µm 3 < Lc.V < 1000 µm 3 , Lc.L1/Lc.L2 < 5, and 0 ≤ Lc.χ ≤ 2.…”

Scaffolds and tissue regeneration: An overview of the functional properties of selected organic tissues

“…65 However, images obtained by confocal microscopy show that osteocytes are located in cavities or gaps of ellipsoidal shape within the bone matrix. 68 The format of osteocytes is characteristic for each type of bone: in the fibula and femur, osteocytes have elongated shapes, while in the skull osteocytes have spherical shapes. 69,70 Osteoblasts synthesize the organic part (type I collagen, proteoglycans, and glycoproteins) and participate in bone mineralization by having the ability to concentrate calcium phosphate.…”