3D patterned substrates for bioartificial blood vessels – The effect of hydrogels on aligned cells on a biomaterial surface

Abstract

Surface features control cellular orientation and subsequently influence their functionality, a useful effect for cellularized biomedical devices. Such devices also can benefit from protective and cell friendly hydrogel coatings. However, literature is lacking on the fate of cells that have endured hydrogel coating whilst orientated on a biomaterial surface. In particular, elucidation of the cells ability to remain adherent and orientated post hydrogel addition. Coating requires two procedures that may be deleterious to the orientated cells: the surface pretreatment for gel binding and the hydrogel crosslinking reaction. We compare transglutaminase gelatin crosslinking and UV initiated PEGDA crosslinking, coated onto smooth muscle cells orientated on patterned PCL surfaces. This original study will be of considerable use to the wider biomaterials community.

Matching Static and Dynamic Compliance of Small‐Diameter Arteries, with Poly(lactide‐co‐caprolactone) Copolymers: In Vitro and In Vivo Studies

“…Although no immediate thrombosis was recorded during the 3 h in vivo, longer durations are required to assess PLC 70:30 tubes on their blood compatibility 98. Even though Poly‐ε‐caprolactone and PLC have been described as having good hemocompatibility,33,101–103 they are not ideal surfaces for blood‐contacting devices. Without any modification, PLC surfaces will absorb protein over time and allow platelet adhesion 33,104,105.…”

Modern opportunities of regenerative medicine: biofabrication of hollow organs

“…Left panel -bladder scaffold and its structural characteristics. Right panel -bladder scaffold after 7 days of cultivation with pig urothelial and smooth muscle cells [54] Кровеносные сосуды могут быть получены различными методами, включая трехмерную печать, трехмерные рисунки на гидрогелях [58] и композитные скаффолды [59][60][61]. Тканевые сосудистые трансплантаты, сконструированные с использованием аутологичных клеток и биодеградируемых каркасов, были применены в исследованиях на моделях ягнят и собак [62].…”

A facile method for fabricating a three-dimensional aligned fibrous scaffold for vascular application

“…8,9 Smooth muscle cells when loses its contractile protein and quiescence state will undergo increased proliferation, migration and produce excessive extracellular matrix (ECM) proteins and pro-inammatory cytokines and growth factors which eventually lead to deadly vascular diseases. 8,10 Several research group have made attempts to modulate the phenotype of vascular smooth muscle cells through approaches such as micro-patterning, 9,11,12 electrospinning, [13][14][15] coating of heparin 8,16 or having endothelial cells (ECs) on the inner side of scaffold. 10,16,17 While micro-patterning creates a dened cellular monolayer of cells, it rapidly loses its regularity when translated into multiple layers.…”