3D plotting of growth factor loaded calcium phosphate cement scaffolds

Akkineni, Ashwini Rahul; Luo, Yongxiang; Schumacher, Matthias; Nies, Berthold; Lode, Anja; Gelinsky, Michael

doi:10.1016/j.actbio.2015.08.036

Cited by 140 publications

(104 citation statements)

References 41 publications

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“…64 Akkineni et al (2015) described a similar approach of extruding α -TCP-based CaP cement (CPC) premixed with chitosan/dextran sulphate microparticles encapsulating vascular endothelial growth factor (VEGF) or bovine serum albumin (BSA) in a liquid carrier consisting of a biocompatible oil. 1 The extruded CPC scaffolds had compressive strength and moduli in the range of the compressive properties of trabecular bone. The bioocompatibility of the scaffolds was demonstrated by the viability and alkaline phosphatase activity of mesenchymal stem cells cultivated on the scaffolds for up 21 days.…”

Section: D Printing For Drug Deliverymentioning

confidence: 98%

3D Printing of Calcium Phosphate Ceramics for Bone Tissue Engineering and Drug Delivery

et al. 2016

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Additive manufacturing, also known as 3D printing, has emerged over the past 3 decades as a disruptive technology for rapid prototyping and manufacturing. Vat polymerization, powder bed fusion, material extrusion, and binder jetting are distinct technologies of additive manufacturing, which have been used in a wide variety of fields, including biomedical research and tissue engineering. The ability to print biocompatible, patient-specific geometries with controlled macro- and micropores, and to incorporate cells, drugs and proteins has made 3D-printing ideal for orthopaedic applications, such as bone grafting. Herein, we performed a systematic review examining the fabrication of calcium phosphate (CaP) ceramics by 3D printing, their biocompatibility in vitro, and their bone regenerative potential in vivo, as well as their use in localized delivery of bioactive molecules or cells. Understanding the advantages and limitations of the different 3D printing approaches, CaP materials, and bioactive additives through critical evaluation of in vitro and in vivo evidence of efficacy is essential for developing new classes of bone graft substitutes that can perform as well as autografts and allografts or even surpass the performance of these clinical standards.

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Section: D Printing For Drug Deliverymentioning

confidence: 98%

3D Printing of Calcium Phosphate Ceramics for Bone Tissue Engineering and Drug Delivery

et al. 2016

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“…(52) Calcium phosphates such as hydroxyapatite, for instance, can be printed alongside osteogenic growth factor patterns to mimic the biochemical distribution of these cues in native bone matrix. (53,54) Several examples exist in the literature where calcium phosphates have been printed in a vertical gradient with camphene,(55) poly(propylene fumarate),(56) and other scaffold materials(23) to generate biochemical distributions and physical architectures that mimic features of natural bone tissue – e.g., the transition from cortical to cancellous bone. (57) Literature is much sparser, however, on the creation of calcium phosphate gradients in combination with growth factor gradients.…”

Section: Fabricating Spatiotemporal Growth Factor Patternsmentioning

confidence: 99%

Spatiotemporal control of growth factors in three-dimensional printed scaffolds

Bittner

Guo

2018

Bioprinting

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Three-dimensional printing (3DP) has enabled the fabrication of tissue engineering scaffolds that recapitulate the physical, architectural, and biochemical cues of native tissue matrix more effectively than ever before. One key component of biomimetic scaffold fabrication is the patterning of growth factors, whose spatial distribution and temporal release profile should ideally match that seen in native tissue development. Tissue engineers have made significant progress in improving the degree of spatiotemporal control over which growth factors are presented within 3DP scaffolds. However, significant limitations remain in terms in pattern resolution, the fabrication of true gradients, temporal control of growth factor release, the maintenance of growth factor distributions against diffusion, and more. This review summarizes several key areas for advancement of the field in terms of improving spatiotemporal control over growth factor presentation, and additionally highlights several major tissues of interest that have been targeted by 3DP growth factor patterning strategies.

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“…However, recently AM technologies have expanded their applications to tissue engineering [32,33]. The next section describes in detail the fundamentals of additive manufacturing and presents some examples of its application on the development of nanocomposite-based scaffolds for tissue engineering.…”

Section: Uctsmentioning

confidence: 99%