3D printable Polycaprolactone-gelatin blends characterized for in vitro osteogenic potency

Azarudeen, Raja S.; Hassan, Mohamad Nageeb; Yassin, Mohammed A.; Thirumarimurugan, M.; Muthukumarasamy, N.; Velauthapillai, Dhayalan; Mustafa, Kamal

doi:10.1016/j.reactfunctpolym.2019.104445

Cited by 16 publications

(15 citation statements)

References 40 publications

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“…Several authors have investigated scaffolds combining various materials to provide successful substitution or mechanical support needed to promote new tissue growth at defect sites. However, the mechanical properties of hybrid scaffolds depend on the method of manufacture, the content, the orientation of the nanomaterials, the nature of the matrix, the interactions between the polymer matrix and the nanomaterials, etc 21,29,[57][58][59][60] . Different filament compositions were used for 3D printing of PLA/CNC scaffolds using FDM as described in the experimental section.…”

Section: Resultsmentioning

confidence: 99%

3D printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineering

N’Gatta

Belaid

Hayek

et al. 2022

Sci Rep

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Cellulose nanocrystals (CNC) are drawing increasing attention in the fields of biomedicine and healthcare owing to their durability, biocompatibility, biodegradability and excellent mechanical properties. Herein, we fabricated using fused deposition modelling technology 3D composite scaffolds from polylactic acid (PLA) and CNC extracted from Ficus thonningii. Scanning electron microscopy revealed that the printed scaffolds exhibit interconnected pores with an estimated average pore size of approximately 400 µm. Incorporating 3% (w/w) of CNC into the composite improved PLA mechanical properties (Young's modulus increased by ~ 30%) and wettability (water contact angle decreased by ~ 17%). The mineralization process of printed scaffolds using simulated body fluid was validated and nucleation of hydroxyapatite confirmed. Additionally, cytocompatibility tests revealed that PLA and CNC-based PLA scaffolds are non-toxic and compatible with bone cells. Our design, based on rapid 3D printing of PLA/CNC composites, combines the ability to control the architecture and provide improved mechanical and biological properties of the scaffolds, which opens perspectives for applications in bone tissue engineering and in regenerative medicine.

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Section: Resultsmentioning

confidence: 99%

3D printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineering

N’Gatta

Belaid

Hayek

et al. 2022

Sci Rep

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“…Chemical bonds of HA, GLU, PCL, MS, and MS-GLU were analyzed by FTIR (Figure C). It can be seen from the figure that the C–H group stretching vibration peaks of PCL were located at 2909 and 2824 cm –1 . The peak at 1720 cm –1 was the stretching vibration peak of the C5O group .…”

Section: Resultsmentioning

confidence: 91%

“…It can be seen from the figure that the C−H group stretching vibration peaks of PCL were located at 2909 and 2824 cm −1 . 46 The peak at 1720 cm −1 was the stretching vibration peak of the C5O group. 47 The O−H flexural vibration peak was located at 1366 cm −1 .…”

Section: Resultsmentioning

confidence: 99%

Core–Shell Nanofibers with a Shish-Kebab Structure Simulating Collagen Fibrils for Bone Tissue Engineering

Ding

Cheng

et al. 2021

ACS Appl. Bio Mater.

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The repair of bone defects is one of the great challenges facing modern orthopedics clinics. Bone tissue engineering scaffold with a nanofibrous structure similar to the original microstructure of a bone is beneficial for bone tissue regeneration. Here, a core–shell nanofibrous membrane (MS), MS containing glucosamine (MS-GLU), MS with a shish-kebab (SK) structure (SKMS), and MS-GLU with a SK structure (SKMS-GLU) were prepared by micro-sol electrospinning technology and a self-induced crystallization method. The diameter of MS nanofibers was 50–900 nm. Contact angle experiments showed that the hydrophilicity of SKMS was moderate, and its contact angle was as low as 72°. SK and GLU have a synergistic effect on cell growth. GLU in the core of MS was demonstrated to obviously promote MC3T3-E1 cell proliferation. At the same time, the SK structure grown on MS-GLU nanofibers mimicked natural collagen fibers, which facilitated MC3T3-E1 cell adhesion and differentiation. This study showed that a biomimetic SKMS-GLU nanofibrous membrane was a promising tissue engineering scaffold for bone defect repair.

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“…The main objective of these studies is to design and synthesize new bioinks derived from natural or fully synthetic polymers which perfectly mimic the desired cellular environment in order to positively influence cell development towards a cell type in a perfectly controlled and reproducible manner. Furthermore, these modifications allow the chemical linking of bioactive molecules, such as growth factors, nanoparticles, micro carriers, peptides that will give additional properties to the bioink, for example, hydroxyapatite grafting could increase gel stiffness and improve cell differentiation in osteoblasts [ 38 ]. In addition, the gelation process of these new bioinks must be perfectly controlled to be able to encapsulate the cells and then to be 3D printed.…”

Section: 3d Bioprintingmentioning

confidence: 99%

In Vitro Human Joint Models Combining Advanced 3D Cell Culture and Cutting-Edge 3D Bioprinting Technologies

Jørgensen

Simon

2021

Cells

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Joint-on-a-chip is a new technology able to replicate the joint functions into microscale systems close to pathophysiological conditions. Recent advances in 3D printing techniques allow the precise control of the architecture of the cellular compartments (including chondrocytes, stromal cells, osteocytes and synoviocytes). These tools integrate fluid circulation, the delivery of growth factors, physical stimulation including oxygen level, external pressure, and mobility. All of these structures must be able to mimic the specific functions of the diarthrodial joint: mobility, biomechanical aspects and cellular interactions. All the elements must be grouped together in space and reorganized in a manner close to the joint organ. This will allow the study of rheumatic disease physiopathology, the development of biomarkers and the screening of new drugs.

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3D printable Polycaprolactone-gelatin blends characterized for in vitro osteogenic potency

Cited by 16 publications

References 40 publications

3D printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineering

3D printing of cellulose nanocrystals based composites to build robust biomimetic scaffolds for bone tissue engineering

Core–Shell Nanofibers with a Shish-Kebab Structure Simulating Collagen Fibrils for Bone Tissue Engineering

In Vitro Human Joint Models Combining Advanced 3D Cell Culture and Cutting-Edge 3D Bioprinting Technologies

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