3D printed bioceramic for phage therapy against bone nosocomial infections

Bouchart, Franck; Vidal, Olivier; Lacroix, Jean-Marie; Spriet, Corentin; Chamary, Shaan; Brutel, Annie; Hornez, Jean‐Christophe

doi:10.1016/j.msec.2020.110840

Cited by 25 publications

(17 citation statements)

References 19 publications

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“…The pellets were then tested for lytic activity in triplicate to determine the effectiveness of this method and to demonstrate the total release of the phages from the β-TCP materials. The prepared alginate/phage solutions did not interfere with the repeatability of the amount of phage loaded on the ceramic pellets, nor the reproducibility, as the results are consistent with previous results obtained by Meurice et al [ 21 ] and Bouchart et al [ 28 ]. The method used to introduce phages into the pellets demonstrated significant reproducibility.…”

Section: Resultssupporting

confidence: 90%

“…A higher initial phage concentration in the pellets also meant a higher lytic activity towards the targeted bacteria at the first moment of contact. This immediate antimicrobial response was also demonstrated by Bouchart et al [ 28 ] against Staphylococcus aureus biofilms.…”

Section: Discussionsupporting

confidence: 74%

“…As shown by microscopy ( Figure 2 ), the layers formed by the alginate hydrogel on the asperities of the pellets could explain the protective action of the hydrogel on the phage load. This property could be interesting when the alginate-covered biomaterials are placed in a fluidic environment, for example in vivo to maintain the lytic activity of the bone substitute and for the safety of osteoblast eukaryotic cells, as suggested by Bouchart et al [ 28 ]. Moreover, the use of alginate did not affect the lytic activity of the phage and led to better lytic efficiency of our biomaterials from the start.…”

Section: Discussionmentioning

confidence: 99%

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A Localized Phage-Based Antimicrobial System: Effect of Alginate on Phage Desorption from β-TCP Ceramic Bone Substitutes

et al. 2020

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Tricalcium phosphate (TCP) is a prosthetic material commonly used as a bone substitute to repair osteoarticular diseases and injuries. In this type of bone reconstruction surgery, antibiotics remain the common preventive and therapeutic treatment for bacterial infection. Nevertheless, the emergence of multi-resistant strains requires complimentary or alternative treatments. Today, one of the promising alternative approaches is phage therapy. Phages are bacterial viruses that have several advantages over chemotherapy, such as the specificity of bacterial strain, the absence of side effects, and a rapid response. In this work, we studied the impact of alginate hydrogels for overlaying λvir-phage-loaded β-TCP ceramic bone substitutes, delaying the phage desorption. The results show that the use of a 1% alginate–CaCl2 hydrogel overlapping the β-TCP ceramic pellets leads to higher initial phage concentration on the material and extends the released time of phages to two weeks when compared with control pellets. These alginate-coated biomaterials also generate faster bacterial lysis kinetics and could therefore be a good practical prosthetic device for bone and joint surgeries by allowing local treatment of bacterial infections with phage therapy for a longer period of time.

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Section: Resultssupporting

confidence: 90%

Section: Discussionsupporting

confidence: 74%

Section: Discussionmentioning

confidence: 99%

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A Localized Phage-Based Antimicrobial System: Effect of Alginate on Phage Desorption from β-TCP Ceramic Bone Substitutes

et al. 2020

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“…Phage therapy and phage lysins have already been proposed as possible agents for treating patients with ventilator-associated pneumonia [110]. In addition, phage therapy, alone or in combination with antibiotics, has been proposed for post-operative joint and bone infections, as biofilm formation in surface biomaterials, mainly by S. aureus, is a major concern [111].…”

Section: Biofilms In Medical Environments and Medical Devicesmentioning

confidence: 99%

Phages for Biofilm Removal

2020

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Biofilms are clusters of bacteria that live in association with surfaces. Their main characteristic is that the bacteria inside the biofilms are attached to other bacterial cells and to the surface by an extracellular polymeric matrix. Biofilms are capable of adhering to a wide variety of surfaces, both biotic and abiotic, including human tissues, medical devices, and other materials. On these surfaces, biofilms represent a major threat causing infectious diseases and economic losses. In addition, current antibiotics and common disinfectants have shown limited ability to remove biofilms adequately, and phage-based treatments are proposed as promising alternatives for biofilm eradication. This review analyzes the main advantages and challenges that phages can offer for the elimination of biofilms, as well as the most important factors to be taken into account in order to design effective phage-based treatments.

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“…Therefore, there is an urgent need to seek and develop new therapeutic mastitis treatments targeting this pathogen. Treatment of bacterial infections by phages is one of the alternative methods [ 8 – 10 ]. Studies indicate that only lytic phages should be used for effective phage therapy [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

and #1057;haracterization of lytic activity of Phage SAvB14 on Staphylococcus aureus variant bovis

Horiuk

Kukhtyn

et al. 2020

J Adv Vet Anim Res

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Objective: The objective of this study was to investigate the intensity of phage infection caused by Phage SAvB14 , which was isolated from dairy farms, depending on the initial number of Staphylococcus aureus cells in the medium. Material and methods: To evaluate the impact of the viable bacteria S. aureus var. bovis on the intensity of phage infection caused by Phage SAvB14 , 1 mg of phagolysate (phage titer 10 5 CFU/ ml) was introduced in 9 ml of nutrient broth with an appropriate amount of daily culture of S. aureus var. bovis under study. The number of viable staphylococci was determined by total viable count/ml. Results: In this experiment, we found that the intensity of phages lytic activity was dependent on the number of sensitive bacterial cells in the volume of the culture medium. Effective phage therapy requires a high concentration of phages in the medium (inflammation foci) for rapid contact of the virus with bacteria. Conclusion: When developing a phage drug to treat subclinical mastitis, it is necessary to increase the phage titer in the drug or its dosage compared to the clinical form, as there is a lower probability of phage contact with a susceptible microbial cell. Besides, at a high concentration of bacteria, there is a gradual decrease in nutrients in the medium, resulting in phages going back to the condition of lysogeny.

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3D printed bioceramic for phage therapy against bone nosocomial infections

Cited by 25 publications

References 19 publications

A Localized Phage-Based Antimicrobial System: Effect of Alginate on Phage Desorption from β-TCP Ceramic Bone Substitutes

A Localized Phage-Based Antimicrobial System: Effect of Alginate on Phage Desorption from β-TCP Ceramic Bone Substitutes

Phages for Biofilm Removal

and #1057;haracterization of lytic activity of Phage SAvB14 on Staphylococcus aureus variant bovis

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