3D-printed fluidic networks as vasculature for engineered tissue

Kinstlinger, Ian S.; Miller, Jordan S.

doi:10.1039/c6lc00193a

Cited by 117 publications

(83 citation statements)

References 158 publications

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“…On the one hand, such progress has arisen from the desire to devise more functional organ-on-chips. 51 For example, mimicking 3D networks of the microvasculature has received growing attention: efforts to recreate in vitro microcirculatory architectures have been motivated by tissue engineering applications as well as vasculo- and angiogenesis studies 52–55 . In parallel, recreating microvascular networks using microfluidics has also inspired novel artificial lung designs and blood oxygenators, as recently reviewed, 56 despite ongoing challenges in meeting physiological in vivo requirements of gas exchange at the whole-organ level.…”

Section: Translating the Pulmonary Anatomy For Microfluidicsmentioning

confidence: 99%

Biomimetics of the pulmonary environment in vitro: A microfluidics perspective

et al. 2018

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The entire luminal surface of the lungs is populated with a complex yet confluent, uninterrupted airway epithelium in conjunction with an extracellular liquid lining layer that creates the air-liquid interface (ALI), a critical feature of healthy lungs. Motivated by lung disease modelling, cytotoxicity studies, and drug delivery assessments amongst other, in vitro setups have been traditionally conducted using macroscopic cultures of isolated airway cells under submerged conditions or instead using transwell inserts with permeable membranes to model the ALI architecture. Yet, such strategies continue to fall short of delivering a sufficiently realistic physiological in vitro airway environment that cohesively integrates at true-scale three essential pillars: morphological constraints (i.e., airway anatomy), physiological conditions (e.g., respiratory airflows), and biological functionality (e.g., cellular makeup). With the advent of microfluidic lung-on-chips, there have been tremendous efforts towards designing biomimetic airway models of the epithelial barrier, including the ALI, and leveraging such in vitro scaffolds as a gateway for pulmonary disease modelling and drug screening assays. Here, we review in vitro platforms mimicking the pulmonary environment and identify ongoing challenges in reconstituting accurate biological airway barriers that still widely prevent microfluidic systems from delivering mainstream assays for the end-user, as compared to macroscale in vitro cell cultures. We further discuss existing hurdles in scaling up current lung-on-chip designs, from single airway models to more physiologically realistic airway environments that are anticipated to deliver increasingly meaningful whole-organ functions, with an outlook on translational and precision medicine.

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Section: Translating the Pulmonary Anatomy For Microfluidicsmentioning

confidence: 99%

Biomimetics of the pulmonary environment in vitro: A microfluidics perspective

et al. 2018

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“…Additive manufacturing (“3D-printing”) may enable designer biomaterial architectures that direct microfluidic flows 1, 2 or functionally interface with living cells. 3, 4 Previously, bulk hydrogels based on crosslinked, hydrophilic polymers have been engineered with spatial or temporal complexity, 5, 6 but this functionality remains nascent for 3D-printed structures.…”

Section: Introductionmentioning

confidence: 99%

“…1 Such microfluidic devices can be utilized to manipulate interfacial transport, including controlled mixing or gradient formation. 51 In particular, gradients within hydrogels can be generated by diffusion between a source and a sink channel.…”

Section: Introductionmentioning

confidence: 99%

Stereolithographic printing of ionically-crosslinked alginate hydrogels for degradable biomaterials and microfluidics

et al. 2017

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3D printed biomaterials with spatial and temporal functionality could enable interfacial manipulation of fluid flows and motile cells. However, such dynamic biomaterials are challenging to implement since they must be responsive to multiple, biocompatible stimuli. Here, we show stereolithographic printing of hydrogels using noncovalent (ionic) crosslinking, which enables reversible patterning with controlled degradation. We demonstrate this approach using sodium alginate, photoacid generators and various combinations of divalent cation salts, which can be used to tune the hydrogel degradation kinetics, pattern fidelity, and mechanical properties. This approach is first utilized to template perfusable microfluidic channels within a second encapsulating hydrogel for T-junction and gradient devices. The presence and degradation of printed alginate microstructures were further verified to have minimal toxicity on epithelial cells. Degradable alginate barriers were used to direct collective cell migration from different initial geometries, revealing differences in front speed and leader cell formation. Overall, this demonstration of 3D printing using non-covalent crosslinking may enable adaptive and stimuli-responsive biomaterials, which could be utilized for bio-inspired sensing, actuation, drug delivery, and tissue engineering.

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“…As a novel and alternative approach, three-dimensional (3D) printing can be used to fabricate microfluidic systems within biomaterials for wound healing applications. [233][234][235] Three-dimensional bioprinting technology is able to provide mechanically strong, scalable, and structurally integrated vascularized systems within biomaterials guiding cells to promote microvascularization.…”

Section: Microfabrication Technologies To Provide Biochemical and Biomentioning

confidence: 99%

Biochemical and Biophysical Cues in Matrix Design for Chronic and Diabetic Wound Treatment

Xiao

Ahadian

Radišić

2017

Tissue Engineering Part B: Reviews

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Progress in biomaterial science and engineering and increasing knowledge in cell biology have enabled us to develop functional biomaterials providing appropriate biochemical and biophysical cues for tissue regeneration applications. Tissue regeneration is particularly important to treat chronic wounds of people with diabetes. Understanding and controlling the cellular microenvironment of the wound tissue are important to improve the wound healing process. In this study, we review different biochemical (e.g., growth factors, peptides, DNA, and RNA) and biophysical (e.g., topographical guidance, pressure, electrical stimulation, and pulsed electromagnetic field) cues providing a functional and instructive acellular matrix to heal diabetic chronic wounds. The biochemical and biophysical signals generally regulate cell-matrix interactions and cell behavior and function inducing the tissue regeneration for chronic wounds. Some technologies and devices have already been developed and used in the clinic employing biochemical and biophysical cues for wound healing applications. These technologies can be integrated with smart biomaterials to deliver therapeutic agents to the wound tissue in a precise and controllable manner. This review provides useful guidance in understanding molecular mechanisms and signals in the healing of diabetic chronic wounds and in designing instructive biomaterials to treat them.

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3D-printed fluidic networks as vasculature for engineered tissue

Cited by 117 publications

References 158 publications

Biomimetics of the pulmonary environment in vitro: A microfluidics perspective

Biomimetics of the pulmonary environment in vitro: A microfluidics perspective

Stereolithographic printing of ionically-crosslinked alginate hydrogels for degradable biomaterials and microfluidics

Biochemical and Biophysical Cues in Matrix Design for Chronic and Diabetic Wound Treatment

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