3D-Printed GelMA/PEGDA/F127DA Scaffolds for Bone Regeneration

Abstract

Tissue-engineered scaffolds are an effective method for the treatment of bone defects, and their structure and function are essential for bone regeneration. Digital light processing (DLP) printing technology has been widely used in bone tissue engineering (BTE) due to its high printing resolution and gentle printing process. As commonly used bioinks, synthetic polymers such as polyethylene glycol diacrylate (PEGDA) and Pluronic F127 diacrylate (F127DA) have satisfactory printability and mechanical properties but usually lack sufficient adhesion to cells and tissues. Here, a compound BTE scaffold based on PEGDA, F127DA, and gelatin methacrylate (GelMA) was successfully prepared using DLP printing technology. The scaffold not only facilitated the adhesion and proliferation of cells, but also effectively promoted the osteogenic differentiation of mesenchymal stem cells in an osteoinductive environment. Moreover, the bone tissue volume/total tissue volume (BV/TV) of the GelMA/PEGDA/F127DA (GPF) scaffold in vivo was 49.75 ± 8.50%, higher than the value of 37.10 ± 7.27% for the PEGDA/F127DA (PF) scaffold and 20.43 ± 2.08% for the blank group. Therefore, the GPF scaffold prepared using DLP printing technology provides a new approach to the treatment of bone defects.

New Photocrosslinked 3D Foamed Scaffolds Based on GelMA Copolymers: Potential Application in Bone Tissue Engineering

“…It should be noted that synthetic polymers will have better mechanical properties while natural polymers are preferred by cells. A widely used strategy is the application of mixtures of GelMa/organic biomaterial, such as polylactic acid, natural extracellular cartilage-derived matrix or organic-derived poly (ester amide) to form in all cases hybrid organic hydrogel scaffolds, which has proven to be an effective strategy for bone tissue regeneration [9,[12][13][14][15].…”

ECM-based bioadhesive hydrogel for sutureless repair of deep anterior corneal defects

“…36 The improved mechanical properties of GelMA hydrogels by addition of PEGDA have been also reported by previous studies. 25,35,[37][38][39] Paper Biomaterials Science…”

From Free Tissue Transfer to Hydrogels: A Brief Review of the Application of the Periosteum in Bone Regeneration

“…These improvements serve to increase the cellular proliferation and differentiation of bone marrow mesenchymal stem cells [ 115 ]. Owing to its excellent printability, GelMA has been well utilized as a cell-laden bioprinting ink for tissue engineering [ 116 ], with recent studies supporting the addition of hydroxyapatite [ 117 ], silica nanoparticles [ 118 ], and synthetic polymers [ 119 ] to improve the mechanical properties and bioactivity of GelMA based bioinks.…”