3D printed hydroxyapatite composite scaffolds with enhanced mechanical properties

Chen, Shangsi; Shi, Yufei; Zhang, Xin; Ma, Jun

doi:10.1016/j.ceramint.2019.02.182

Cited by 59 publications

(38 citation statements)

References 24 publications

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“…A comparison between the values obtained in this study and the values found in the literature is shown in Table 1. It is important to note that a cancellous allograft bone presents as the best option in bone surgery, being called a golden standard by specialists [60]—a finding that corroborates the results found in this study for the S HG scaffold.…”

Section: Resultssupporting

confidence: 89%

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Development of Composite Scaffolds Based on Cerium Doped-Hydroxyapatite and Natural Gums—Biological and Mechanical Properties

et al. 2019

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Hydroxyapatite (HAp) is a ceramic material composing the inorganic portion of bones. Ionic substitutions enhance characteristics of HAp, for example, calcium ions (Ca2+) by cerium ions (Ce3+). The use of HAp is potentialized through biopolymers, cashew gum (CG), and gellan gum (GG), since CG/GG is structuring agents in the modeling of structured biocomposites, scaffolds. Ce-HApCG biocomposite was synthesized using a chemical precipitation method. The obtained material was frozen (–20 °C for 24 h), and then vacuum dried for 24 h. The Ce-HApCG was characterized by X-Ray diffractograms (XRD), X-ray photoemission spectra (XPS), Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), and energy dispersive spectroscopy (EDS). XRD and FTIR showed that Ce-HApCG was successfully synthesized. XRD showed characteristic peaks at 2θ = 25.87 and 32.05, corresponding to the crystalline planes (0 0 2) and (2 1 1), respectively, while phosphate bands were present at 1050 cm−1 and 1098 cm−1, indicating the success of composite synthesis. FESEM showed pores and incorporated nanostructured granules of Ce-HApCG. The mechanical test identified that Ce-HApCG has a compressive strength similar to the cancellous bone’s strength and some allografts used in surgical procedures. In vitro tests (MTT assay and hemolysis) showed that scaffold was non-toxic and exhibited low hemolytic activity. Thus, the Ce-HApCG has potential for application in bone tissue engineering.

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Section: Resultssupporting

confidence: 89%

“…The studies indicate a great diversity in pore size in the scaffolds synthesized with the size variation occurring in the range of 10 to 1000 μm. This variation of pore size occurs due to the different bone morphologies found in the human body, as well as in the scaffold application sites [60,61,62,63].…”

Section: Resultsmentioning

confidence: 99%

Development of Composite Scaffolds Based on Cerium Doped-Hydroxyapatite and Natural Gums—Biological and Mechanical Properties

et al. 2019

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“…The low temperature 3D printing technique would not damage the structure and component of prepared Mg‐HA crystal, which means it would make the best use of biocompatible and bioactive Col I and CA (Lin et al, ). To make printing paste, we utilized Gel as biobinder to bind Mg‐HA particles together, and added a small part of CHI to enhance the mechanical property of prepared scaffolds (Chen, Shi, Zhang, & Ma, ). Both Gel (Kuo et al, ) and CHI (Venkatesan & Kim, ) are good candidates for fabricating bone tissue engineering scaffolds, and their composite scaffolds were also reported to support bone regeneration (Georgopoulou et al, ).…”

Section: Discussionmentioning

confidence: 99%

Biomimetic synthesis of Mg‐substituted hydroxyapatite nanocomposites and three‐dimensional printing of composite scaffolds for bone regeneration

Chen

Shi

Zhang

et al. 2019

J Biomedical Materials Res

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In this study, we have successfully fabricated magnesium (Mg) substituted hydroxyapatite nanocomposites (Mg-HA) by utilizing type I collagen (COL I) and citric acid (CA) through a bitemplate-induced biomimetic mineralization approach. The obtained composite nanoparticles were subsequently mixed with chitosan (CHI) and gelatin (Gel) to prepare porous scaffolds with interconnected structures by three-dimensional (3D) printing technique. The Mg-HA powders and composite scaffolds were characterized. The results showed that the substitution of Mg for Ca ions reduced the crystallinity of HA crystals, but did not significantly affect the size and structure of the nanocomposites. The morphology of Mg-HA scaffolds turned smoother compared with the HA scaffolds with Mg substitution. Furthermore, the biocompatibility of Mg-HA composite scaffolds was evaluated by metal ion release, cell attachment, proliferation, and differentiation of MC3T3-E1 cells.According to the results, as the more Ca 2+ was substituted by Mg 2+ , the more Mg 2+ was released from the samples and the pH in cultured medium was more acidic. It was suggested that Mg-HA scaffolds presented higher cell attachment, proliferation rate, increased expression of alkaline phosphatase (ALP) activity and osteogenic related gene, including osteocalcin (OCN), runt-related transcription factor 2 (RUNX2), and COL I. Therefore, it was indicated that the 3D printed Mg-HA composite scaffolds with excellent biocompatibility and bioactivity were a potential candidate in bone tissue engineering.

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“…Chen et al developed 3D-printed composite scaffolds composed of HAp, gelatin, chitosan, and carboxymethyl cellulose using a Regenovo Bioprinter (Regenovo Biotechnology, China); these scaffolds showed ∼600 µm macroporosity and the ultimate compressive strength of 14.3 MPa for optimized composite scaffold (Chen et al, 2019a). In another study, a trilayered scaffold has been fabricated using extrusion-based multinozzle 3D printing for the regeneration of cartilage and subchondral bone simultaneously.…”

Section: Hap/mixed Components Printed Materialsmentioning

confidence: 99%

Additive Manufacturing Methods for Producing Hydroxyapatite and Hydroxyapatite-Based Composite Scaffolds: A Review

et al. 2019

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Hydroxyapatite (HAp) has been considered for decades an ideal biomaterial for bone repair due to its compositional and crystallographic similarity to bioapatites in hard tissues. However, fabrication of porous HAp acting as a template (scaffold) for supporting bone regeneration and growth has been a challenge to biomaterials scientists. The introduction of additive manufacturing technologies, which provide the advantages of a relatively fast, precise, controllable, and potentially scalable fabrication process, has opened new horizons in the field of bioceramic scaffolds. This review focuses on three-dimensional-printed HAp scaffolds and related composite systems, where the calcium phosphate phase is combined with other ceramics or polymers improving the mechanical properties and/or imparting special extra-functionalities. The main applications of three-dimensional-printed HAp scaffolds in bone tissue engineering are presented and discussed; furthermore, this review also emphasizes the most recent achievements toward the development and testing of multifunctional HAp-based systems combining multiple properties for advanced therapy (e.g., bone regeneration, antibacterial effect, angiogenesis, and cancer treatment).

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3D printed hydroxyapatite composite scaffolds with enhanced mechanical properties

Cited by 59 publications

References 24 publications

Development of Composite Scaffolds Based on Cerium Doped-Hydroxyapatite and Natural Gums—Biological and Mechanical Properties

Development of Composite Scaffolds Based on Cerium Doped-Hydroxyapatite and Natural Gums—Biological and Mechanical Properties

Biomimetic synthesis of Mg‐substituted hydroxyapatite nanocomposites and three‐dimensional printing of composite scaffolds for bone regeneration

Additive Manufacturing Methods for Producing Hydroxyapatite and Hydroxyapatite-Based Composite Scaffolds: A Review

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