3D printed microfluidic devices for lipid bilayer recordings

Ogishi, Kazuto; Osaki, Toshihisa; Morimoto, Yuya; Takeuchi, Shoji

doi:10.1039/d1lc01077h

Cited by 21 publications

(12 citation statements)

References 41 publications

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“…Here, we introduced the step to control the direction of bubbles but also considered the use of a hydrophobic/hydrophilic patterning of the surfaces that would similarly guide the bubbles by the surface tension forces. However, we avoided the negative influence of the surface modification on the formation process of BLM, 41 where infusions of both aqueous solution and organic solvent into the wells are mandatory.…”

Section: Resultsmentioning

confidence: 99%

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Reproducible reformation of a bilayer lipid membrane using microair bubbles

Hashimoto

Osaki

Sugiura

et al. 2023

Droplet

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Planar bilayer lipid membranes (BLMs) are widely used as models for cell membranes in various applications, including drug discovery and biosensors. However, the nanometer‐thick bilayer structure, assembled through hydrophobic interactions of amphiphilic lipid molecules, makes such BLM systems mechanically and electrically unstable. In this study, we developed a device to reform BLMs using a microair bubble. The device consists of a double well divided by a separator with a microaperture, where a BLM was formed by infusing a lipid‐dispersed solvent and an aqueous droplet into each well in series. When the BLM ruptured, a microair bubble was injected from the bottom of the well to split the merged aqueous droplet at the microaperture, which resulted in the reformation of two lipid monolayers on the split droplets. By bringing the two droplets into contact, a new BLM was formed. An angled step design was introduced in the BLM device to guide the bubble and ensure the splitting of the merged droplet. We also elucidated the optimal bubble inflow rate for the reproducible BLM reformation. Using a 4‐channel parallel device, we demonstrated the individual and repeatable reformation of BLMs. Our approach will aid the development of automated and arrayed BLM systems.

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Section: Resultsmentioning

confidence: 99%

“…To verify the BLM reformation with air bubbles, a 4ch device was fabricated using a three‐dimensional printer (XFAB3500; DWS) 41 . The device design was the same as the one above.…”

Section: Methodsmentioning

confidence: 99%

Reproducible reformation of a bilayer lipid membrane using microair bubbles

Hashimoto

Osaki

Sugiura

et al. 2023

Droplet

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“…Several groups have reported the integration of nanopores within microfluidic architectures for high-throughput detection of nanoparticles. − Varongchayakul et al presented a standalone microfluidic-nanopore setup that supports on-chip sample preparation, purification, and single-molecule nanopore measurements (Figure E) . With the recent development in 3D-printing technology, nanopore supports and microdevices with custom features can now be tailored in a rapid and facile manner, favoring their integration in miniaturized setups. Roman et al reported a PDMS-based microfluidic device with an integrated nanopore utilizing 3D-printed molds …”

Section: High-throughput Nanoporesmentioning

confidence: 99%

The New Era of High-Throughput Nanoelectrochemistry

Valavanis

Ciocci

et al. 2023

Anal. Chem.

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“…[72][73][74] Varongchayakul et al presented a standalone microfluidic-nanopore setup that supports on-chip sample preparation, purification and singlemolecule nanopore measurements (Figure 1E). 64 With the recent development in 3D-printing technology, nanopore supports and microdevices with custom features can now be tailored in a rapid and facile manner, 75 favoring their integration in miniaturized setups. Roman et al reported a PDMS-based microfluidic device with integrated nanopore utilizing 3D-printed moulds.…”

Section: Integration Modalities For Nanoporesmentioning

confidence: 99%

The new era of high throughput nanoelectrochemistry

Valavanis

Ciocci

et al. 2022

Preprint

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Scanning electrochemical probe microscopies (SEPMs) have played a key role in advancing small-scale electrochemistry. SEPMs use an electrochemical probe (micro/nanoelectrode or pipet) to quantify and map local interfacial fluxes of electroactive species and have found increasingly wide applications. Our contribution to the Fundamental and Applied Reviews in Analytical Chemistry 2019 discussed how advances in SEPMs converged towards nanoscale electrochemical mapping. This inflection in experimental capability has opened up myriad opportunities for SEPMs in many types of systems, from material and energy sciences to the life sciences. The enhancement in the spatial resolution of imaging techniques, and instrumental developments, have resulted in significant increases in the size of electrochemical datasets from a typical experiment and served to speed up measurement throughput. Next generation nanoelectrochemistry will thus see an emphasis on “big data”, its analysis, storage and curation, high throughput analysis and parallelization, “intelligent” instruments and experiments, active control of nanoscale systems, and the integration of nanoelectrochemistry and nanoscale micro(spectro)scopy. For this review article, we focus on recent advances in frontier nanoscale electrochemistry, analysis and imaging techniques that are already addressing some of these key targets, and are well-placed to embrace other aspects in the near future. Our goal is to provide an overview of the present state-of-the-art in high throughput nanoelectrochemistry and imaging, and signpost promising new avenues for nanoscale electrochemical methods.

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3D printed microfluidic devices for lipid bilayer recordings

Abstract: This paper verifies the single-step and monolithic fabrication of 3D structured lipid bilayer devices using stereolithography. Lipid bilayer devices are utilized to host membrane proteins in vitro for biological assays...

Cited by 21 publications

References 41 publications

Reproducible reformation of a bilayer lipid membrane using microair bubbles

Reproducible reformation of a bilayer lipid membrane using microair bubbles

The New Era of High-Throughput Nanoelectrochemistry

The new era of high throughput nanoelectrochemistry

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