3D printed microfluidic mixer for point-of-care diagnosis of anemia

Plevniak, Kimberly; Campbell, Matthew; He, Mei

doi:10.1109/embc.2016.7590691

Cited by 6 publications

(3 citation statements)

References 64 publications

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“…The mixing process within SARS microchannels takes place Lastly, the Serpentine channel geometry (Figure 5d,h) shows an efficient mixing between the continuous phase and the dispersed phase with a mixing time of approximately 7.95 ± 0.92 s and an efficient of 99.58 ± 0.13% in silico and 77.96 ± 1.49% in vitro, and a ME/∆P of 1.71 ± 0.06. This can be attributed to the serpentine's microchannel length, which allows the two phases to stay longer within the turns of the device [81,82]. This allows the streamlines to help to maintain a continuous phase interaction, favoring simple diffusion.…”

Section: Encapsulation By Direct Interactionmentioning

confidence: 99%

Design and Manufacture of a Low-Cost Microfluidic System for the Synthesis of Giant Liposomes for the Encapsulation of Yeast Homologues: Applications in the Screening of Membrane-Active Peptide Libraries

et al. 2021

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The discovery of new membrane-active peptides (MAPs) is an area of considerable interest in modern biotechnology considering their ample applicability in several fields ranging from the development of novel delivery vehicles (via cell-penetrating peptides) to responding to the latent threat of antibiotic resistance (via antimicrobial peptides). Different strategies have been devised for such discovery process, however, most of them involve costly, tedious, and low-efficiency methods. We have recently proposed an alternative route based on constructing a non-rationally designed library recombinantly expressed on the yeasts’ surfaces. However, a major challenge is to conduct a robust and high-throughput screening of possible candidates with membrane activity. Here, we addressed this issue by putting forward low-cost microfluidic platforms for both the synthesis of Giant Unilamellar Vesicles (GUVs) as mimicking entities of cell membranes and for providing intimate contact between GUVs and homologues of yeasts expressing MAPs. The homologues were chitosan microparticles functionalized with the membrane translocating peptide Buforin II, while intimate contact was through passive micromixers with different channel geometries. Both microfluidic platforms were evaluated both in silico (via Multiphysics simulations) and in vitro with a high agreement between the two approaches. Large and stable GUVs (5–100 µm) were synthesized effectively, and the mixing processes were comprehensively studied leading to finding the best operating parameters. A serpentine micromixer equipped with circular features showed the highest average encapsulation efficiencies, which was explained by the unique mixing patterns achieved within the device. The microfluidic devices developed here demonstrate high potential as platforms for the discovery of novel MAPs as well as for other applications in the biomedical field such as the encapsulation and controlled delivery of bioactive compounds.

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Section: Encapsulation By Direct Interactionmentioning

confidence: 99%

Design and Manufacture of a Low-Cost Microfluidic System for the Synthesis of Giant Liposomes for the Encapsulation of Yeast Homologues: Applications in the Screening of Membrane-Active Peptide Libraries

et al. 2021

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“…Plevniak et al [ 42 ] demonstrated a 3D printed microfluidic mixer for fast mixing of reagents with blood through capillary force. The device was integrated with a smartphone for the point-of-care diagnosis of anemia from a finger-prick blood sample.…”

Section: Continuous Flow Microfluidicsmentioning

confidence: 99%

Recent Advances and Future Perspectives on Microfluidic Liquid Handling

et al. 2017

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The interdisciplinary research field of microfluidics has the potential to revolutionize current technologies that require the handling of a small amount of fluid, a fast response, low costs and automation. Microfluidic platforms that handle small amounts of liquid have been categorised as continuous-flow microfluidics and digital microfluidics. The first part of this paper discusses the recent advances of the two main and opposing applications of liquid handling in continuous-flow microfluidics: mixing and separation. Mixing and separation are essential steps in most lab-on-a-chip platforms, as sample preparation and detection are required for a variety of biological and chemical assays. The second part discusses the various digital microfluidic strategies, based on droplets and liquid marbles, for the manipulation of discrete microdroplets. More advanced digital microfluidic devices combining electrowetting with other techniques are also introduced. The applications of the emerging field of liquid-marble-based digital microfluidics are also highlighted. Finally, future perspectives on microfluidic liquid handling are discussed.

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“…The design of such systems can be first explored in silico, with the aid of several methods including rapid and economic CAD prototyping [36,37], hydraulic circuit analyses [38,39], and computational fluid dynamics (CFD) [40][41][42][43][44][45]. CFD simulations of the micromixers allow the rapid design and testing of the performance of multiple prototypes, by identifying the variables that most impact the efficiency and overall performance of the device for a particular task [40,42,43].…”

Section: Introductionmentioning

confidence: 99%

CFD Analysis and Life Cycle Assessment of Continuous Synthesis of Magnetite Nanoparticles Using 2D and 3D Micromixers

et al. 2022

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Magnetite nanoparticles (MNPs) have attracted basic and applied research due to their immense potential to enable applications in fields as varied as drug delivery and bioremediation. Conventional synthesis schemes led to wide particle size distributions and inhomogeneous morphologies and crystalline structures. This has been attributed to the inability to control nucleation and growth processes under the conventional conditions of bulk batch processes. Here, we attempted to address these issues by scaling down the synthesis process aided by microfluidic devices, as they provide highly controlled and stable mixing patterns. Accordingly, we proposed three micromixers with different channel configurations, namely, serpentine, triangular, and a 3D arrangement with abrupt changes in fluid direction. The micromixers were first studied in silico, aided by Comsol Multiphysics® to investigate the obtained mixing patterns, and consequently, their potential for controlled growth and the nucleation processes required to form MNPs of uniform size and crystalline structure. The devices were then manufactured using a low-cost approach based on polymethyl methacrylate (PMMA) and laser cutting. Testing the micromixers in the synthesis of MNPs revealed homogeneous morphologies and particle size distributions, and the typical crystalline structure reported previously. A life cycle assessment (LCA) analysis for the devices was conducted in comparison with conventional batch co-precipitation synthesis to investigate the potential impacts on water and energy consumption. The obtained results revealed that such consumptions are higher than those of the conventional process. However, they can be reduced by conducting the synthesis with reused micromixers, as new PMMA is not needed for their assembly prior to operation. We are certain that the proposed approach represents an advantageous alternative to co-precipitation synthesis schemes, in terms of continuous production and more homogeneous physicochemical parameters of interest such as size, morphologies, and crystalline structure. Future work should be directed towards improving the sustainability indicators of the micromixers’ manufacturing process.

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3D printed microfluidic mixer for point-of-care diagnosis of anemia

Cited by 6 publications

References 64 publications

Design and Manufacture of a Low-Cost Microfluidic System for the Synthesis of Giant Liposomes for the Encapsulation of Yeast Homologues: Applications in the Screening of Membrane-Active Peptide Libraries

Design and Manufacture of a Low-Cost Microfluidic System for the Synthesis of Giant Liposomes for the Encapsulation of Yeast Homologues: Applications in the Screening of Membrane-Active Peptide Libraries

Recent Advances and Future Perspectives on Microfluidic Liquid Handling

CFD Analysis and Life Cycle Assessment of Continuous Synthesis of Magnetite Nanoparticles Using 2D and 3D Micromixers

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