3D printed PCL/SrHA scaffold for enhanced bone regeneration

Liu, Dinghua; Nie, Wei; Li, Dejian; Wang, Weizhong; Zheng, Lixia; Zhang, Jingtian; Zhang, Jiulong; Peng, Chen; Mo, Xiumei; He, Chuanglong

doi:10.1016/j.cej.2019.01.015

Cited by 204 publications

(144 citation statements)

References 25 publications

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“…More recently, by employing strontium–HA compounds, Liu et al prepared 3D‐printed scaffolds with different percentages of reinforcement (0:100 to 50:50 wt%) to test in vitro and in vivo the capability of the constructs to promote bone regeneration. The samples showed a compressive stress on the order of 7 MPa with E of 40 MPa with fair biological outcomes revealing the proliferation and the osteogenic differentiation of the bone marrow–derived stem cells (BMSCs) seeded into them …”

Section: Hard Matrix‐based Compositesmentioning

confidence: 99%

Additive Manufacturing Approaches for Hydroxyapatite‐Reinforced Composites

Milazzo

Negrini

Scialla

et al. 2019

Adv Funct Materials

140

113

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Additive manufacturing (AM) techniques have gained interest in the tissue engineering field, thanks to their versatility and unique possibilities of producing constructs with complex macroscopic geometries and defined patterns. Recently, composite materials-namely, heterogeneous biomaterials identified as continuous phase (matrix) and reinforcement (filler)-have been proposed as inks that can be processed by AM to obtain scaffolds with improved biomimetic and bioactive properties. Significant efforts have been dedicated to hydroxyapatite (HA)-reinforced composites, especially targeting bone tissue engineering, thanks to the chemical similarities of HA with respect to mineral components of native mineralized tissues. Herein, applications of AM techniques to process HA-reinforced composites and biocomposites for the production of scaffolds with biological matrices, including cellular tissues, are reviewed. The primary outcomes of recent investigations in terms of morphological, structural, and in vitro and in vivo biological properties of the materials are discussed. The approaches based on the nature of the matrices employed to embed the HA reinforcements and produce the tissue substitutes are classified, and a critical discussion is provided on the presented state of the art as well as the future perspectives, to offer a comprehensive picture of the strategies investigated as well as challenges in this emerging field of materiomics.

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Section: Hard Matrix‐based Compositesmentioning

confidence: 99%

Additive Manufacturing Approaches for Hydroxyapatite‐Reinforced Composites

Milazzo

Negrini

Scialla

et al. 2019

Adv Funct Materials

140

113

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“…The composite scaffolds were developed by using a PCL polymeric matrix mainly for its good printability, biodegradability and for being an FDA approved biomaterial [ 49 ]. In relation to the ceramic phase, previous studies on Sr-containing biomaterials in various forms indicated that even small amounts of Sr (0.1 wt%) can enhance the osteoconductive properties of calcium phosphate, have a positive effect on bone mineralization, elevate the mechanical property of bone tissue and have crucial effects by inducing collagen type I synthesis [ 50 , 51 , 52 , 53 ]. Additionally, it has been reported that Sr can act via the calcium-sensing receptor and promote the transductions of the mitogen-activated protein kinase signaling.…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, as shown in the higher magnification images, cell–cell contact and filopodia formation was established particularly on the composite-based samples ( Figure 9 H,I), whereas on the PCL scaffold surface cells displayed a more rounded shape and the filopodia were almost indiscernible. This outcome was mainly ascribed to the scaffold composition, since it is widely reported as the essential role of nHA and Sr-containing nHA to promote attachment, proliferation and osteoblastic differentiation [ 34 , 50 , 53 ]. Furthermore, the actual roughness of the as printed composite scaffolds’ (see Figure 4 E–F) could be identified as another reason determining the positive cell–substrate interaction informed in Figure 9 [ 71 , 72 , 73 ].…”

Section: Resultsmentioning

confidence: 99%

Towards 3D Multi-Layer Scaffolds for Periodontal Tissue Engineering Applications: Addressing Manufacturing and Architectural Challenges

et al. 2020

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Reduced periodontal support, deriving from chronic inflammatory conditions, such as periodontitis, is one of the main causes of tooth loss. The use of dental implants for the replacement of missing teeth has attracted growing interest as a standard procedure in clinical practice. However, adequate bone volume and soft tissue augmentation at the site of the implant are important prerequisites for successful implant positioning as well as proper functional and aesthetic reconstruction of patients. Three-dimensional (3D) scaffolds have greatly contributed to solve most of the challenges that traditional solutions (i.e., autografts, allografts and xenografts) posed. Nevertheless, mimicking the complex architecture and functionality of the periodontal tissue represents still a great challenge. In this study, a porous poly(ε-caprolactone) (PCL) and Sr-doped nano hydroxyapatite (Sr-nHA) with a multi-layer structure was produced via a single-step additive manufacturing (AM) process, as a potential strategy for hard periodontal tissue regeneration. Physicochemical characterization was conducted in order to evaluate the overall scaffold architecture, topography, as well as porosity with respect to the original CAD model. Furthermore, compressive tests were performed to assess the mechanical properties of the resulting multi-layer structure. Finally, in vitro biological performance, in terms of biocompatibility and osteogenic potential, was evaluated by using human osteosarcoma cells. The manufacturing route used in this work revealed a highly versatile method to fabricate 3D multi-layer scaffolds with porosity levels as well as mechanical properties within the range of dentoalveolar bone tissue. Moreover, the single step process allowed the achievement of an excellent integrity among the different layers of the scaffold. In vitro tests suggested the promising role of the ceramic phase within the polymeric matrix towards bone mineralization processes. Overall, the results of this study demonstrate that the approach undertaken may serve as a platform for future advances in 3D multi-layer and patient-specific strategies that may better address complex periodontal tissue defects.

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“…Polycaprolactone (PCL) is widely used in tissue engineering involving engineering and natural science [1]. When the biological material and manufacturing method developed, its usage caught much attention [2][3][4]. PCL's good bio-compatibility and compatibility with other polymers, makes it a favorable material for sca olds, especially in the biomanufacturing and chemical industries [5,6].…”

Section: Introductionmentioning

confidence: 99%

“…PCL's weak melt-strength means it cannot be extruded into laments. erefore, pure PCL always fails to meet the requirement of traditional FDM technology [3,22]. Some biomaterials get bad in uences from the step of making into laments [23].…”

Section: Introductionmentioning

confidence: 99%

A Pellet 3D Printer: Device Design and Process Parameters Optimization

Liu

Zhao

et al. 2019

Advances in Polymer Technology

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A novel pellet 3D printer was first developed, and its structure was constructed out of three main parts. The material used in this device was polycaprolactone (PCL), which was praised for its good characteristics in the biomanufacturing and chemical industries. Three essential parameters that had important effects on the diameter of the printed fibers were systematically studied using a L9(34) orthogonal design table. Using the fused deposition modelling (FDM) method, some products were printed with this machine. Results showed that the stepper motor’s speed had the most significant effect on the diameter of the printed fibers. The optimal parameters were, a stepper motor speed of 1.256 mm3 s−1, a nozzle moving speed of 9.6 mm s−1, and 1.1 mm of height between the nozzle and the platform. Defects like gaps, warping, and poor surface quality were found to be related to different combinations of process parameters. By using the developed pellet 3D printer, the pre-step of making filaments can be avoided, which will bring convenience to FDM 3D printing.

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3D printed PCL/SrHA scaffold for enhanced bone regeneration

Cited by 204 publications

References 25 publications

Additive Manufacturing Approaches for Hydroxyapatite‐Reinforced Composites

Additive Manufacturing Approaches for Hydroxyapatite‐Reinforced Composites

Towards 3D Multi-Layer Scaffolds for Periodontal Tissue Engineering Applications: Addressing Manufacturing and Architectural Challenges

A Pellet 3D Printer: Device Design and Process Parameters Optimization

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