3D printed polycaprolactone/beta-tricalcium phosphate/magnesium peroxide oxygen releasing scaffold enhances osteogenesis and implanted BMSCs survival in repairing the large bone defect

Peng, Ziyue; Wang, Chengqiang; Li, Chun; Xu, Haixia; Wang, Yihan; Liu, Yang; Hu, Yun-Teng; Li, Jianjun; Jin, Yuqi; Jiang, Cong; Liu, Liangle; Guo, Jiasong; Zhu, Lixin

doi:10.1039/d1tb00178g

Cited by 41 publications

(31 citation statements)

References 38 publications

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“…Severe hypoxia usually suppresses proliferation and osteogenic differentiation of BMSCs and impedes bone defect reconstruction [ 28 ]. In the treatment of large bone defects, ischemia and hypoxia in the bone defect area remain a thorny issue [ 29 ]. Here, hypoxic BMSCs presented increased apoptosis and oxidative stress and reduced mitochondrial activity.…”

Section: Discussionmentioning

confidence: 99%

Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT Signaling

Liang

Zhou

Liu

et al. 2022

Oxidative Medicine and Cellular Longevity

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Objective. Transplantation of bone marrow-derived mesenchymal stem cells (BMSCs) remains a hopeful therapeutic approach for bone defect reconstruction. Herein, we investigated the effects and mechanisms of leukemia inhibitory factor (LIF) in the function and viability of hypoxic BMSCs as well as bone defect repair. Methods. The effects of LIF on apoptosis (flow cytometry, TUNEL staining), mitochondrial activity (JC-1 staining), proliferation (colony formation, EdU staining), and differentiation (CD105, CD90, and CD29 via flow sorting) were examined in hypoxic BMSCs. LIF, LIFR, gp130, Keap1, Nrf2, antioxidant enzymes (SOD1, catalase, GPx-3), bone-specific matrix proteins (ALP, BSP, OCN), PI3K, and Akt were detected via immunoblotting or immunofluorescent staining. BMSCs combined with biphasic calcium phosphate scaffolds were implanted into calvarial bone defect mice, and the therapeutic effect of LIF on bone defect was investigated. Results. Hypoxic BMSCs had increased apoptosis and oxidative stress and reduced mitochondrial activity. Additionally, LIF, LIFR, and gp130 were upregulated and PI3K/Akt activity was depressed in hypoxic BMSCs. Upregulated LIF alleviated apoptosis and oxidative stress and heightened mitochondrial activity and PI3K/Akt signaling in hypoxic BMSCs. Additionally, LIF overexpression promoted self-renewal and osteogenic differentiation of BMSCs with hypoxic condition. Mechanically, LIF facilitated self-renewal and differentiation as well as attenuated oxidative stress of BMSCs through enhancing PI3K/AKT signaling activity. Implantation of LIF-overexpressed BMSC-loaded BCP scaffolds promoted osteogenesis as well as alleviated oxidative stress and apoptosis through PI3K/Akt signaling. Conclusion. Our findings demonstrate that LIF facilitates self-renewal and differentiation and attenuates oxidative stress of BMSCs by PI3K/AKT signaling.

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Section: Discussionmentioning

confidence: 99%

Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT Signaling

Liang

Zhou

Liu

et al. 2022

Oxidative Medicine and Cellular Longevity

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“…Sr 2+ enhances the synthesis of VEGF and ANG1 in MSCs [ 94 ]. Moreover, Ca 2+ , Mg 2+ , Zn 2+ and Sr 2+ are each shown to promote the migration [ [103] , [104] , [105] ] and osteo-differentiation [ 27 , [30] , [31] , [32] , [33] , 94 ] of MSCs. However, the collaborative action of the multi-ions on MSC migration and angio/osteo-genic response remains elusive, especially in the presence of MΦs.…”

Section: Resultsmentioning

confidence: 99%

Cubic multi-ions-doped Na2TiO3 nanorod-like coatings: Structure-stable, highly efficient platform for ions-exchanged release to immunomodulatory promotion on vascularized bone apposition

et al. 2022

Bioactive Materials

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“…64,81,82,65,5 Peng et al fabricated the PCL/β-TCP/MgO 2 scaffold and found that when the weight ratio of MgO 2 was 10%, the scaffold's Young's modulus was 102.01 MPa, matching the property of natural bones and nearly twice that of the control group containing no MgO 2 or MgO powder. 82 Zhang et al reported that adding magnesium as well as POSS synergistically enhanced the GelMA/TCS hydrogel's tensile strength by almost 2-fold and reached 310.8 ± 11.5 kPa. 81 Gu et al found the young's modulus and maximum load stress of TCP scaffold containing 1 wt % MgO were 930.65 ± 163.79 MPa and 30.69 ± 3.90 MPa, respectively, which were much higher than that of the pure TCP scaffold.…”

Section: Effects Of Magnesium Ions On Bone Tissue Engineeringmentioning

confidence: 99%

Effects of Zinc, Magnesium, and Iron Ions on Bone Tissue Engineering

Chen

Zhang

Wang

et al. 2022

ACS Biomater. Sci. Eng.

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Large-sized bone defects are a great challenge in clinics and considerably impair the quality of patients’ daily life. Tissue engineering strategies using cells, scaffolds, and bioactive molecules to regulate the microenvironment in bone regeneration is a promising approach. Zinc, magnesium, and iron ions are natural elements in bone tissue and participate in many physiological processes of bone metabolism and therefore have great potential for bone tissue engineering and regeneration. In this review, we performed a systematic analysis on the effects of zinc, magnesium, and iron ions in bone tissue engineering. We focus on the role of these ions in properties of scaffolds (mechanical strength, degradation, osteogenesis, antibacterial properties, etc.). We hope that our summary of the current research achievements and our notifications of potential strategies to improve the effects of zinc, magnesium, and iron ions in scaffolds for bone repair and regeneration will find new inspiration and breakthroughs to inspire future research.

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3D printed polycaprolactone/beta-tricalcium phosphate/magnesium peroxide oxygen releasing scaffold enhances osteogenesis and implanted BMSCs survival in repairing the large bone defect

Abstract: Fabricate a MgO2-contained scaffold by 3D printing to improve ischemia and hypoxia in bone defect area.

Cited by 41 publications

References 38 publications

Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT Signaling

Leukemia Inhibitory Factor Facilitates Self-Renewal and Differentiation and Attenuates Oxidative Stress of BMSCs by Activating PI3K/AKT Signaling

Cubic multi-ions-doped Na2TiO3 nanorod-like coatings: Structure-stable, highly efficient platform for ions-exchanged release to immunomodulatory promotion on vascularized bone apposition

Effects of Zinc, Magnesium, and Iron Ions on Bone Tissue Engineering

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