3D printed polylactide scaffolding for laccase immobilization to improve enzyme stability and estrogen removal from wastewater

Rybarczyk, Agnieszka; Smułek, Wojciech; Grzywaczyk, Adam; Kaczorek, Ewa; Jesionowski, Teofil; Nghiem, Long D.; Zdarta, Jakub

doi:10.1016/j.biortech.2023.129144

Cited by 25 publications

(3 citation statements)

References 42 publications

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“…They have many hydroxyl groups, which make them efficient support materials for immobilized enzymes. Recently, 3D printing has been reported as being used to directly produce various support materials for enzyme immobilization [30], and 3D printing has the advantage of enabling the accurate manufacture of complex support structures in short periods without the use of harmful solvents [31,32].…”

Section: Immobilization Methods For Enzyme-immobilized Reactorsmentioning

confidence: 99%

Bioremediation of Hazardous Pollutants Using Enzyme-Immobilized Reactors

Yamaguchi,

Miyazaki

2024

Molecules

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Bioremediation uses the degradation abilities of microorganisms and other organisms to remove harmful pollutants that pollute the natural environment, helping return it to a natural state that is free of harmful substances. Organism-derived enzymes can degrade and eliminate a variety of pollutants and transform them into non-toxic forms; as such, they are expected to be used in bioremediation. However, since enzymes are proteins, the low operational stability and catalytic efficiency of free enzyme-based degradation systems need improvement. Enzyme immobilization methods are often used to overcome these challenges. Several enzyme immobilization methods have been applied to improve operational stability and reduce remediation costs. Herein, we review recent advancements in immobilized enzymes for bioremediation and summarize the methods for preparing immobilized enzymes for use as catalysts and in pollutant degradation systems. Additionally, the advantages, limitations, and future perspectives of immobilized enzymes in bioremediation are discussed.

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Section: Immobilization Methods For Enzyme-immobilized Reactorsmentioning

confidence: 99%

Bioremediation of Hazardous Pollutants Using Enzyme-Immobilized Reactors

Yamaguchi,

Miyazaki

2024

Molecules

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“…A piece of work using 3D bioprinting utilized a bioink made of sodium alginate, acrylamide, and hydroxyapatite with immobilized laccase for biodegradation of p-chlorophenol where the immobilized laccase exhibited excellent storage stability and reusability and retained over 80% of its initial enzyme activity after three days of storage, and was able to be reused for treating seven batches of phenolic compounds ( Liu et al, 2020 ). Another recent work using laccase reported a biocatalytic system using immobilized laccase to 3D printed open-structure biopolymer scaffolds that were shown to remove 35-40% of estrogen group hormones such as 17β-estradiol and 17α-ethynylestradiol from municipal wastewater containing 56 ng/L of 17α-ethynylestradiol and 187 ng/L of 17β-estradiol ( Rybarczyk et al, 2023 ). Research has also demonstrated that these estrogen group hormones could bind onto 3D-printed (SLS) filters made from commonly used polymers, such as polyamide-12 (PA), thermoplastic polyurethane (TPU), polypropylene (PP), and polystyrene (PS), and these filters showed enhanced surface morphology ( Fig.…”

Section: Applications Of 3d Printing In Bioremediationmentioning

confidence: 99%

3D bioprinting in bioremediation: a comprehensive review of principles, applications, and future directions

Finny

2024

PeerJ

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Bioremediation is experiencing a paradigm shift by integrating three-dimensional (3D) bioprinting. This transformative approach augments the precision and versatility of engineering with the functional capabilities of material science to create environmental restoration strategies. This comprehensive review elucidates the foundational principles of 3D bioprinting technology for bioremediation, its current applications in bioremediation, and the prospective avenues for future research and technological evolution, emphasizing the intersection of additive manufacturing, functionalized biosystems, and environmental remediation; this review delineates how 3D bioprinting can tailor bioremediation apparatus to maximize pollutant degradation and removal. Innovations in biofabrication have yielded bio-based and biodegradable materials conducive to microbial proliferation and pollutant sequestration, thereby addressing contamination and adhering to sustainability precepts. The review presents an in-depth analysis of the application of 3D bioprinted constructs in enhancing bioremediation efforts, exemplifying the synergy between biological systems and engineered solutions. Concurrently, the review critically addresses the inherent challenges of incorporating 3D bioprinted materials into diverse ecological settings, including assessing their environmental impact, durability, and integration into large-scale bioremediation projects. Future perspectives discussed encompass the exploration of novel biocompatible materials, the automation of bioremediation, and the convergence of 3D bioprinting with cutting-edge fields such as nanotechnology and other emerging fields. This article posits 3D bioprinting as a cornerstone of next-generation bioremediation practices, offering scalable, customizable, and potentially greener solutions for reclaiming contaminated environments. Through this review, stakeholders in environmental science, engineering, and technology are provided with a critical appraisal of the current state of 3D bioprinting in bioremediation and its potential to drive forward the efficacy of environmental management practices.

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“…The utilization of protease enzymatic digestion for peptide preparation has gained growing focus due to its safety and controllability, but then comes the drawback that the free enzyme is unstable and not recyclable [ 19 ]. Immobilized enzymes, which are widely used in the food industry, have more stable enzyme properties and can be recycled after the reaction is completed [ 20 ], showing great potential for Se-enriched peptide preparation.…”

Section: Introductionmentioning

confidence: 99%

Co-Immobilization of Alcalase/Dispase for Production of Selenium-Enriched Peptide from Cardamine violifolia

Zhu,

Li,

Chen

et al. 2024

Foods

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Enzymatically derived selenium-enriched peptides from Cardamine violifolia (CV) can serve as valuable selenium supplements. However, the industrial application of free enzyme is impeded by its limited stability and reusability. Herein, this study explores the application of co-immobilized enzymes (Alcalase and Dispase) on amino resin for hydrolyzing CV proteins to produce selenium-enriched peptides. The successful enzyme immobilization was confirmed through scanning electron microscopy (SEM), energy dispersive X-ray (EDX), and Fourier-transform infrared spectroscopy (FTIR). Co-immobilized enzyme at a mass ratio of 5:1 (Alcalase/Dispase) exhibited the smallest pore size (7.065 nm) and highest activity (41 U/mg), resulting in a high degree of hydrolysis of CV protein (27.2%), which was obviously higher than the case of using free enzymes (20.7%) or immobilized Alcalase (25.8%). In addition, after a month of storage, the co-immobilized enzyme still retained a viability level of 41.93%, showing fairly good stability. Encouragingly, the selenium-enriched peptides from co-immobilized enzyme hydrolysis exhibited uniform distribution of selenium forms, complete amino acid fractions and homogeneous distribution of molecular weight, confirming the practicality of using co-immobilized enzymes for CV protein hydrolysis.

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3D printed polylactide scaffolding for laccase immobilization to improve enzyme stability and estrogen removal from wastewater

Cited by 25 publications

References 42 publications

Bioremediation of Hazardous Pollutants Using Enzyme-Immobilized Reactors

Bioremediation of Hazardous Pollutants Using Enzyme-Immobilized Reactors

3D bioprinting in bioremediation: a comprehensive review of principles, applications, and future directions

Co-Immobilization of Alcalase/Dispase for Production of Selenium-Enriched Peptide from Cardamine violifolia

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