Abstract3D bioprinting technology is widely used in biomedical fields such as tissue regeneration and constructing pathological model. The prevailing printing technique is extrusion‐based bioprinting. In this printing method, the bioink needs to meet both printability and functionality, which are often conflicting requirements. Therefore, this study has developed an innovative microvalve‐based equipment, incorporating components such as pressure control, a three‐dimensional motion platform, and microvalve. Here, we present a droplet‐based method for constructing complex three‐dimensional structures. By leveraging the rapid switching characteristics of the microvalve, this equipment can achieve precise printing of bio‐materials with viscosities as low as 10mPa·s, significantly expanding the biofabrication window for bioinks. This technology is of great significance for 3D bioprinting in tissue engineering and lays a solid foundation for the construction of complex artificial organ tissues.