3D Printing: 3D‐Printed Artificial Microfish (Adv. Mater. 30/2015)

Zhu, Wei; Li, Jinxing; Leong, Yew J.; Rozen, Isaac; Qu, Xin; Dong, Renfeng; Wu, Zhiguang; Gao, Wei; Chung, Peter; Wang, Joseph; Chen, Shaochen

doi:10.1002/adma.201570200

Cited by 3 publications

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Temperature and osmolality are critical parameters for mammalian cell culture and easy to monitor in industry [50,51]. As a result, hypothermia and hyperosmotic stress have been the two most widely used means to enhance qP in the biopharmaceutical manufacturing process [10,52].…”

Section: Activity Of Ga and Pcmentioning

confidence: 99%

The relationship of metabolic burden to productivity levels in CHO cell lines

Zou

Edros

Al‐Rubeai

2017

Biotech and App Biochem

View full text Add to dashboard Cite

The growing demand for recombinant therapeutics has driven biotechnologists to develop new production strategies. One such strategy for increasing the expression of heterologous proteins has focused on enhancing cell-specific productivity through environmental perturbations. In this work, the effects of hypothermia, hyperosmolarity, high shear stress, and sodium butyrate treatment on growth and productivity were studied using three (low, medium, and high producing) CHO cell lines that differed in their specific productivities of monoclonal antibody. In all three cell lines, the inhibitory effect of these parameters on proliferation was demonstrated. Additionally, compared to the control, specific productivity was enhanced under all conditions and exhibited a consistent cell line specific pattern, with maximum increases (50-290%) in the low producer, and minimum increases (7-20%) in the high producer. Thus, the high-producing cell line was less responsive to environmental perturbations than the low-producing cell line. We hypothesize that this difference is most likely due to the bottleneck associated with a higher metabolic burden caused by higher antibody expression. Increased recombinant mRNA levels and pyruvate carboxylase activities due to low temperature and hyperosmotic stress were found to be positively associated with the metabolic burden.

show abstract

Section: Activity Of Ga and Pcmentioning

confidence: 99%

The relationship of metabolic burden to productivity levels in CHO cell lines

Zou

Edros

Al‐Rubeai

2017

Biotech and App Biochem

View full text Add to dashboard Cite

show abstract

Coaxial 3D bioprinting of tri‐polymer scaffolds to improve the osteogenic and vasculogenic potential of cells in co‐culture models

Shahabipour

Tavafoghi

Aninwene

et al. 2022

J Biomedical Materials Res

View full text Add to dashboard Cite

The crosstalk between osteoblasts and endothelial cells is critical for bone vascularization and regeneration. Here, we used a coaxial 3D bioprinting method to directly print an osteon-like structure by depositing angiogenic and osteogenic bioinks from the core and shell regions of the coaxial nozzle, respectively. The bioinks were made up of gelatin, gelatin methacryloyl (GelMA), alginate, and hydroxyapatite (HAp) nanoparticles and were loaded with human umbilical vascular endothelial cells (HUVECs) and osteoblasts (MC3T3) in the core and shell regions, respectively. Conventional monoaxial 3D bioprinting was used as a control method, where the hydrogels, HAp nanoparticles, MC3T3 cells, and HUVECs were all mixed in one bioink and printed from the core nozzle. As a result, the bioprinted scaffolds were composed of cell-laden fibers with either a core-shell or homogenous structure, providing a noncontact (indirect) or contact (direct) co-culture of MC3T3 cells and HUVECs, respectively. Both structures supported the 3D culture of HUVECs and osteoblasts over a long period. The scaffolds also supported the expression of osteogenic and angiogenic factors. However, the gene expression was significantly higher for the coreshell structure than the homogeneous structure due to the well-defined distribution of osteoblasts and endothelial cells and the formation of vessel-like structures in the co-culture system. Our results indicated that the coaxial bioprinting technique, with the ability to create a non-contact co-culture of cells, can provide a more efficient bioprinting strategy for printing highly vascularized and bioactive bone structures.

show abstract