3D printing and enzyme immobilization: An overview of current trends

Remonatto, Daniela; Izidoro, Bárbara Fernandes; Mazziero, Vítor Teixeira; Cerri, Marcel O.; Paula, Ariela Veloso de

doi:10.1016/j.bprint.2023.e00289

Cited by 11 publications

(3 citation statements)

References 115 publications

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“…Enzymes are highly specific and selective molecules which are involved in different biological processes. Thus, the interest in using printed enzyme-responsive materials for biomedical applications has increased over the years since many enzymes are found in the body [ 180 , 181 , 182 ]. For example, the incorporation of enzymes to 3D-printed materials can be used in the field of tissue regeneration to customize devices that fit patients well or induce the degradation of implants for their natural removal.…”

Section: Stimulus and Effect On Responsive Materialsmentioning

confidence: 99%

4D Printing: The Development of Responsive Materials Using 3D-Printing Technology

Antezana,

Municoy,

Ostapchuk

et al. 2023

Pharmaceutics

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Additive manufacturing, widely known as 3D printing, has revolutionized the production of biomaterials. While conventional 3D-printed structures are perceived as static, 4D printing introduces the ability to fabricate materials capable of self-transforming their configuration or function over time in response to external stimuli such as temperature, light, or electric field. This transformative technology has garnered significant attention in the field of biomedical engineering due to its potential to address limitations associated with traditional therapies. Here, we delve into an in-depth review of 4D-printing systems, exploring their diverse biomedical applications and meticulously evaluating their advantages and disadvantages. We emphasize the novelty of this review paper by highlighting the latest advancements and emerging trends in 4D-printing technology, particularly in the context of biomedical applications.

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Section: Stimulus and Effect On Responsive Materialsmentioning

confidence: 99%

4D Printing: The Development of Responsive Materials Using 3D-Printing Technology

Antezana,

Municoy,

Ostapchuk

et al. 2023

Pharmaceutics

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“…They represent a promising class of bioactive materials for applications in biocatalytic reactors, [25][26][27] sensors 23,[28][29][30] but also as an additive to aid tissue engineering. 18 They are ideal candidates for adding functionality to biocatalytic hydrogels, due to their biocompatibility and high substrate specificity and selectivity, respectively.…”

Section: Introductionmentioning

confidence: 99%

Enzyme Bioink for the 3D Printing of Biocatalytic Materials

Altevogt,

Sheikh,

Molley

et al. 2024

Preprint

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The field of 3D biofabrication faces major challenges on the road to printing fully functional tissues and organs. One of them is adding functionality to the newly formed tissue for replicating an active biochemical environment. Native extracellular matrices sequester numerous bioactive species, making the microenvironment biochemically active. On the other hand, most 3D-printed constructs have limited activity, serving merely as mechanical scaffolding. Here we demonstrate active scaffolding through the integration of biocatalytic enzymes within the bioink. Enzymes are an attractive class of biocompatible and substrate-specific bioactive agents that can improve tissue regeneration outcomes. However, the difficulty in the application remains in providing enzymes at the targeted site in adequate amounts over an extended time.In this work, a durable biocatalytic active enzyme bioink for 3D extrusion-based bioprinting is developed by covalently attaching the globular enzyme horseradish peroxidase (HRP) to a gelatin methacrylate (Gel-MA) biopolymer scaffold. Upon introducing methacrylate groups on the surface of the enzyme, it undergoes photo-crosslinking in a post-printing step with the methacrylate groups of Gel-MA without compromising its activity. As a result, HRP becomes a fixed part of the hydrogel network and achieves higher stability inside the gel which results in a higher concentration and catalytic activity for a longer time than solely entrapping the protein inside the hydrogel. We also demonstrate the cytocompatibility of this enzyme bioink and show its printing capabilities for precise applications in the field of tissue engineering. Our approach offers a promising solution to enhance the bioactive properties of 3D-printed constructs, representing a critical step towards achieving functional biofabricated tissues.

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“…3D printing, or additive manufacturing, has recently come to light as a facile bottom-up manufacturing strategy that allows the swift prototyping and manufacturing of complex geometries . Several sophisticated continuous flow (CF) reactor designs have been successfully fabricated via 3D printing, such as the development of microreactors that incorporate enzyme immobilization and microfluidic technology and modular scaffolding structures that can be assembled as an independent enzymatic reactor or as a filler into an existing enzymatic reactor like the stirred tank reactor. − Cellulase was previously immobilized on a microreactor made of a polytetrafluoroethylene (PTFE) capillary column with the codeposition of dopamine (DA) and polyethylenimine (PEI). The glucose production from this microreactor was enhanced by up to 97.2% in Turnover Frequency (TOF) compared to cellulase immobilized on nanoparticles, showcasing better throughputs via the swift passive mixing by the flowing action of the substrate. , On the other hand, including lattices in a reactor offers improved surface area-to-volume ratio, facilitates enzyme recovery, and eases enzyme–product separation.…”

Section: Introductionmentioning

confidence: 99%

Facile Multilayer Deposition of Polyethylenimine and κ-Carrageenan on a 3D-Printed Lattice for Cellulase Immobilization

Tiong,

Tan,

Foo

et al. 2024

ACS Sustainable Chem. Eng.

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3D printing and enzyme immobilization: An overview of current trends

Cited by 11 publications

References 115 publications

4D Printing: The Development of Responsive Materials Using 3D-Printing Technology

4D Printing: The Development of Responsive Materials Using 3D-Printing Technology

Enzyme Bioink for the 3D Printing of Biocatalytic Materials

Facile Multilayer Deposition of Polyethylenimine and κ-Carrageenan on a 3D-Printed Lattice for Cellulase Immobilization

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