3D printing of complex GelMA-based scaffolds with nanoclay

Gao, Qing; Niu, Xuefeng; Shao, Lei; Zhou, Luyu; Lin, Zhiwei; Sun, Anyu; Fu, Jianzhong; Chen, Zichen; Hu, Jun; Liu, Yande; He, Yong

doi:10.1088/1758-5090/ab0cf6

Cited by 192 publications

(158 citation statements)

References 31 publications

Supporting

Mentioning

155

Contrasting

Unclassified

Order By: Relevance

“…1F). All these results suggested that both mechanical and oriented gradients could be regulated through tuning multiple factors simply, superior to gradient hydrogel systems reported previously (Wang et al, 2018b;Yang et al, 2018;Gao et al, 2019). The mechanical gradients of the hydrogels could be cover different tissues including skin, muscle, cartilage and bone, suggesting their promising applications across these tissues (Nonoyama et al, 2016;Oh et al, 2016;Lu et al, 2018;Yin et al, 2018).…”

Section: Research Articlementioning

confidence: 80%

“…These approaches usually need special apparatus and rigorous parameters and are only feasible for specific materials. Photopatterning is also used widely to introduce both physical and chemical gradients in which photoresponsivity is a prerequisite for the treated systems (Gao et al, 2019). Recently, buoyancy-driven gradients of various cargos were achieved for different biomaterials, suggesting a more versatile strategy of fabricating bioactive biomaterials used in complex tissue regenerations (Li et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…However, finer regulations of these gradients are still required to optimize the functional recovery of tissues. Although plenty of approaches have been developed to form biomaterial gradients (Wang et al, 2018a;Yang et al, 2018;Yin et al, 2018;Gao et al, 2019;Li et al, 2019), little studies could introduce the gradients of multiple cargos simultaneously due to the interference of different cargos in fabrication processes. A gap remains for the biomaterials with gradients and native microenvironments of tissues in vivo.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Electric field-driven building blocks for introducing multiple gradients to hydrogels

Ding

Lü

et al. 2020

Protein Cell

View full text Add to dashboard Cite

Gradient biomaterials are considered as preferable matrices for tissue engineering due to better simulation of native tissues. The introduction of gradient cues usually needs special equipment and complex process but is only effective to limited biomaterials. Incorporation of multiple gradients in the hydrogels remains challenges. Here, betasheet rich silk nanofibers (BSNF) were used as building blocks to introduce multiple gradients into different hydrogel systems through the joint action of crosslinking and electric field. The blocks migrated to the anode along the electric field and gradually stagnated due to the solution-hydrogel transition of the systems, finally achieving gradient distribution of the blocks in the formed hydrogels. The gradient distribution of the blocks could be tuned easily through changing different factors such as solution viscosity, which resulted in highly tunable gradient of mechanical cues. The blocks were also aligned under the electric field, endowing orientation gradient simultaneously. Different cargos could be loaded on the blocks and form gradient cues through the same crosslinking-electric field strategy. The building blocks could be introduced to various hydrogels such as Gelatin and NIPAM, indicating the universality. Complex niches with multiple gradient cues could be achieved through the strategy. Silk-based hydrogels with suitable mechanical gradients were fabricated to control the osteogenesis and chondrogenesis. Chondrogenic-osteogenic gradient transition was obtained, which stimulated the ectopic osteochondral tissue regeneration in vivo. The versatility and highly controllability of the strategy as well as multifunction of the building blocks reveal the applicability in complex tissue engineering and various interfacial tissues.

show abstract

Section: Research Articlementioning

confidence: 80%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electric field-driven building blocks for introducing multiple gradients to hydrogels

Ding

Lü

et al. 2020

Protein Cell

View full text Add to dashboard Cite

show abstract

“…Gelatin combined with other hydrogels has been used to optimize printability, improving shape retention during printing. For example, gelatin-alginate [240,[319][320][321], Laponite [322], nanoclays [323], and synthetic polymers such as Pluronic F-127 [208] and PCL [324], have all been studied. Alginate dialdehyde-gelatin scaffolds were printed in the presence of a cross-linker for reaching feature sizes of~500 µm [245], while alginate-GelMA interpenetrating networks via UV crosslinking of GelMA followed by Ca crosslinking of alginate was reported [321].…”

Section: Structural and Mechanical Propertiesmentioning

confidence: 99%

“…Inclusion of alginate [240,[319][320][321], Laponite [322], nanoclays [323], Pluronic F-127 [208], PCL [324]; Feature size 500 µm [245] [ [313][314][315][316]; Feature size 300 µm (SLA), Compressive E: 0.5-18 MPa [328] Mouse planta dermis [319]; dental pulp stem cells [320]; hMSCs and amniotic epithelial cells [240]; chondrocytes [214,327] Silk ( Mouse articular chondrocytes [342]; human fibroblasts [338]; porcine chondrocytes [340]; hMSCs [344,345]; human mesenchymal progenitor cells [346]…”

Section: ) Applicationsmentioning

confidence: 99%

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

2019

View full text Add to dashboard Cite

The realization of biomimetic microenvironments for cell biology applications such as organ-on-chip, in vitro drug screening, and tissue engineering is one of the most fascinating research areas in the field of bioengineering. The continuous evolution of additive manufacturing techniques provides the tools to engineer these architectures at different scales. Moreover, it is now possible to tailor their biomechanical and topological properties while taking inspiration from the characteristics of the extracellular matrix, the three-dimensional scaffold in which cells proliferate, migrate, and differentiate. In such context, there is therefore a continuous quest for synthetic and nature-derived composite materials that must hold biocompatible, biodegradable, bioactive features and also be compatible with the envisioned fabrication strategy. The structure of the current review is intended to provide to both micro-engineers and cell biologists a comparative overview of the characteristics, advantages, and drawbacks of the major 3D printing techniques, the most promising biomaterials candidates, and the trade-offs that must be considered in order to replicate the properties of natural microenvironments.

show abstract

3D Printing Approaches

2022

3D Printing of Foods

View full text Add to dashboard Cite

3D printing of complex GelMA-based scaffolds with nanoclay

Cited by 192 publications

References 31 publications

Electric field-driven building blocks for introducing multiple gradients to hydrogels

Electric field-driven building blocks for introducing multiple gradients to hydrogels

Engineered 3D Polymer and Hydrogel Microenvironments for Cell Culture Applications

3D Printing Approaches

Contact Info

Product

Resources

About